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Ochrana klienta bankovních (pojišťovacích, investičních) služeb
Konečná, Jitka
This diploma thesis is dealing with issues of consumer protection in area of bank services, namely in area of protection of a depositor and protection of a debtor. Theoretical part deals with legal protection of bank consumers in Czech Republic and in EU, ethical code and financial literacy of consumers. Practical part is divided into two parts; first one is devoted to specific cases widened by compiled results of a questionnaire survey. Second part is dealing with a test of financial literacy. The survey is analysed in this thesis. There will be a set of recommendations regarding bank consumer protection at the conclusion.
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Eating behaviour and personality traits of obese patients depending on BMI
Smutná, Šarlota ; Konečná, Judita (advisor) ; Žůrková, Pavla (referee)
This thesis analyses relationships and interdependencies between eating behaviour, perso- nality traits, and BMI in obese patients. In 2019, the prevalence of obesity was alarming in the Czech Republic, 77 % of working men and 56 % of working women were overweight or obese. The basis of successful treatment is to know patients very well, their history, indi- vidual habits and characteristics that led to the development of obesity. Eating behaviour and personality traits are important parts of this knowledge. They can help us to under- stand the mechanisms of obesity's development and thus also contribute to efective and individually adjusted treatment. We study eating behaviour by the Three-Factor Eating Questionnaire and personality traits by the Personality Inventory for DSM-5 and Beck's Depression and Anxiety Inventory in 598 obese patients (average BMI 44 kg/m2). We split the sample into multiple groups according to the values of BMI to test the diferences between these groups with various methods. Together with correlation coefcients and a linear regression model, we evaluate eating behaviour and personality traits' infuence on BMI. The most important fndings are signifcant diferences between women and men, various correlations of BMI to some personality traits and interdependency...
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Sacred Choir Music by Vít Clar
Konečná, Jolana ; Slavický, Tomáš (advisor) ; Douša, Eduard (referee)
- 48 - 9. RESUME Bakalářská práce je zaměřena na život a dílo Víta Clara. V práci byly použity ukázky ze sborové duchovní tvorby, byly shrnuty kompoziční prostředky. V závěru práce je zhodnocení vybraných skladeb z hlediska interpretačního. Pozornost byla věnována významným českým osobnostem současnosti v české hudební pedagogice posledních desetiletí. Nemalou překážkou byl stav pramenů a nedostatek dohledatelných materiálů, stejně tak i čekání na zpracování notové pozůstalosti. Naopak byly potěšitelné postřehy a odpovědi kolegů a Clarových studentů, zejména jejich milé vzpomínky na nezapomenutelného skladatele a pedagoga. Tato práce je věnována památce Víta Clara im memoriam. 9. RESUME This thesis focuses on the life and work of Vít Clar. It presents some examples of sacred music for choir and summarizes Clar's musical composition elements. The last chapter evaluates selected compositions from the interpretation point of view. This text also pays attention to significant Czech personalities in contemporary musical education of several past decades. Work on this thesis was considerably hampered by the poor conditions of resources and the lack of available materials as well as by the duration of Clar's score estate's processing. On the other hand, observations and answers of Clar's colleagues and students,...
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Use of Molecular Biology Techniques for Identification and Analysis of Probiotic Bacteria
Konečná, Jana ; Doškař, Jiří (referee) ; Kráčmar, Stanislav (referee) ; Obruča, Stanislav (advisor)
Isolation of deoxyribonucleic acid (DNA) is an important step in the molecular diagnostics of microorganisms. A high quality of isolated DNA is necessary for DNA amplification by the polymerase chain reaction (PCR). The conventional DNA isolation using phenol-chloroform extraction and DNA precipitation in ethanol is time-consuming and requires the use of toxic phenol. Alternative method of DNA isolation is use of commercially available kits which, however, are expensive and their efficiency is low. Magnetic separation techniques using magnetic solid particles are one of modern methods to speed up the nucleic acids isolation. The aim of this work was to use two different types of magnetic particles for solid-phase DNA extraction. Magnetic microparticles P(HEMA – co – GMA) containing –NH2 group and nanoparticles PLL, whitch contains polylysine. The amounts of DNA in separation mixtures were measured using ultraviolet spectrophotometry (UV). The first experimental conditions were tested on chicken erythrocytes DNA. Phosphate buffer (pH 7, 7.6 and 8) was used for adsorption of DNA on magnetic particles. It was shown that approximately almost one half of DNA was adsorbed on the particles. The elution conditions of DNA were also optimized. Secondly, bacterial DNA was tested. After optimalization, the developed method was used for DNA isolation from real food supplements. This DNA eluted from the particles was in PCR ready quality. High resolution melting (HRM) curve analysis is a simple, low-cost method for amplicon discrimination and easy connection with real-time polymerase chain reaction (PCR). In this thesis, we report rapid species identification of strains belonging to the Lactobacillus group using HRM-PCR. Three different DNA isolation methods were used in this work: phenol extraction, separation using magnetic particles and commercial kit. Ten sets of targeted gene fragments primers (LAC1 – LAC2, LAC2 – LAC4, P1V1 – P2V1, Gro F – Gro R, 3BA-338f – Primer 1, V1F – V1R, CHAU - V3F – CHAU - V3R, CHAU - V6F – CHAU - V6R, poxcDNAFw – poxPromRVC, poxcDNAFw – poxPromRVT) were tested for amplification of the 16S rRNA gene. Use of GroF/R and LAC2/4 primers pairs successfully identify strains belong to the Lactobacillus group. The variance between used extraction methods for evidence of HRM curves was found.
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PROBIOTIC GENES OF SIGNIFICANT LACTIC ACID BACTERIA IN FOOD
Konečná, Jana ; Ševčovičová,, Andrea (referee) ; Doškař, Jiří (referee) ; Španová, Alena (advisor)
Isolation of deoxyribonucleic acid (DNA) is an important step in the molecular diagnostics of microorganisms. A high quality of isolated DNA is necessary for DNA amplification by the polymerase chain reaction (PCR). The conventional DNA isolation using phenol chloroform extraction and DNA precipitation in ethanol is time consuming and requires the use of toxic phenol. Magnetic separation techniques using magnetic solid particles are one of modern methods to speed up the nucleic acids isolation. The aim of this work was to use two different types of magnetic particles for solidphase DNA extraction. The amounts of DNA in separation mixtures were measured using ultraviolet spectrophotometry (UV). The first experimental conditions were tested on chicken erythrocytes DNA. Phosphate buffer (pH 7, 7.6 and 8) was used for adsorption of DNA on magnetic particles. It was shown that approximately almost one half of DNA was adsorbed to the particles. The elution conditions of DNA were also optimized. Secondly, bacterial DNA was tested. This DNA eluted from the particles was in PCR ready quality. High resolution melting (HRM) curve analysis is a simple, low-cost method for amplicon discrimination and easy connection with real-time polymerase chain reaction (PCR). In this contribution, we report rapid species identification of strains belonging to the Lactobacillus group using HRM-PCR. Three different DNA isolation methods were used in this work: phenol extraction, separation using magnetic particles and commercial kit. Ten sets of targeted gene fragments primers (LAC1 – LAC2, LAC2 – LAC4, P1V1 – P2V1, Gro F – Gro R, 3BA-338f – Primer 1, V1F – V1R, CHAU - V3F – CHAU - V3R, CHAU - V6F – CHAU - V6R, poxcDNAFw – poxPromRVC, poxcDNAFw – poxPromRVT) were tested for amplification of the 16S rRNA gene. Use of GroF/R and LAC2/4 primers pairs successfully identify strains belong to the Lactobacillus group. The variance between used extraction methods for evidence of HRM curves was found.
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Magteic particles and their applications in biotechnology
Knápková, Monika ; Konečná, Jana (referee) ; Trachtová, Štěpánka (advisor)
The thesis is focused on the magnetic particles which are used in several biotechnological applications. The theoretical part deals with the specific properties of these nanoparticles and materials of which the nanoparticles can be made. There are also mentioned some of the biotechnological applications of magnetic particles. During the experimental part, selected types of magnetic particles were used to isolate nucleic acid. The quality of the isolated DNA with respect to purity was evaluated using the polymerase chain reaction and its modifications. High resolution analysis (HRM analysis) was also used to verify the quality of the isolated DNA and to resolution Lactobacillus casei and Lactobacillus rhamnosus. DNA isolation using magnetic carriers was successful. Commercially available MPG microcarriers and magnetic microparticles Fkol 77ox were the most suitable. In terms of purity magnetic nanoparticles F79/L3-PLL were the most suitable for the DNA isolation. The resolution of bacterial strains of Lactobacillus casei and Lactobacillus rhamnosus was not successful.
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Nurses' duty of conifidentiality and the consequences of its breach
Benešová, Pavla ; Prošková, Eva (advisor) ; Konečná, Jana (referee)
The aim of my thesis was to find out what is the legal awareness of nurses in the issue of confidentiality. Furthermore, it was examined whether nurses know what are the consequences for unauthorized access to the medical records. The theoretical part of this thesis describes the mandatory confidentiality issue and its consequences. And it defines the concepts of personal and sensitive personal data. In addition the conditions under which it is required to break confidentiality and the consequences of breach of legal obligation are mentioned. Describe the legal responsibility of nurses. In the practical part of the work I use qualitative methods of data collection, interview, for me to respond to major and minor research questions. I use interviews conducted with nurses, for doing analysis of the case and to describe the measures taken by the employer at the Municipal Hospital in Litoměřice.
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