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Identification of metabolites of anthelminthics in helminth parasites using mass spectrometry
Komrska, Jan ; Skálová, Lenka (advisor) ; Vokřál, Ivan (referee)
If we want be successful in the treatment of parasitic disease, it is important to get the utmost information on the fate of given drug. The discovery of the metabolism of anthelmintics and in cosequence of participating enzymes can help us fight the rising resistance to given drugs. The aim of our work was to find and identify phase I and phase II metabolites of the anthelminthic drugs albendazole (ABZ), flubendazole (FLU) and mebendazole (MEB) formed in ex vivo incubations by parasitic helminth Dicrocoelium dendriticum, using liquid chromatography-mass spectrometric techniques. In the ex vivo study, D. dendriticum adults were incubated in cultivating medium in presence of benzimidazole drug for 24 hours. After incubation of parasites, both parasite homogenates and medium from the incubation were separately extracted using solid phase extraction. The extracts were analyzed using liquid chromatography-mass spectrometry (LC-MS). We detected in phase I albendazole sulphoxide, reduced flubendazole and reduced mebendazole. As for phase II metabolites, methylderivatives of flubendazole, reduced flubendazole and reduced mebendazole were observed.
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Identification of metabolites of anthelminthics in helminth parasites using mass spectrometry
Komrska, Jan ; Vokřál, Ivan (referee) ; Skálová, Lenka (advisor)
If we want be successful in the treatment of parasitic disease, it is important to get the utmost information on the fate of given drug. The discovery of the metabolism of anthelmintics and in cosequence of participating enzymes can help us fight the rising resistance to given drugs. The aim of our work was to find and identify phase I and phase II metabolites of the anthelminthic drugs albendazole (ABZ), flubendazole (FLU) and mebendazole (MEB) formed in ex vivo incubations by parasitic helminth Dicrocoelium dendriticum, using liquid chromatography-mass spectrometric techniques. In the ex vivo study, D. dendriticum adults were incubated in cultivating medium in presence of benzimidazole drug for 24 hours. After incubation of parasites, both parasite homogenates and medium from the incubation were separately extracted using solid phase extraction. The extracts were analyzed using liquid chromatography-mass spectrometry (LC-MS). We detected in phase I albendazole sulphoxide, reduced flubendazole and reduced mebendazole. As for phase II metabolites, methylderivatives of flubendazole, reduced flubendazole and reduced mebendazole were observed.
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Internationalization of business activities of SMEs
Straka, Jakub ; Sato, Alexej (advisor) ; Komrska, Jan (referee)
This Master Thesis is focused on the topic of internationalization of small and medium-sized enterprises (SMEs). The main goal of this thesis was to find out if the czech SMEs are able to participate in international trade, how can they achieve that and if the provided support of SMEs is sufficient. The theoretical part describes the term small and medium-sized enterprises, its specifics, significance and the evolution of the SME sector in Czech Republic. The theoretical part further deals with particular aspects of internationalization and with the quality of the provided support of SMEs in Czech Republic. The practical part was realized in the form of semistructured interview with a owner of Simplejohn, inc and the main goal of it was to verify the conclusions reached in the theoretical part.
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Typology of Urban Tissues Identified in Slovak Cities
Závracká, Andreja ; Komrska, Jan ; Moučka, Jan
The research project 1995 – 1996 was granted by RSS - Research Support Scheme, HESP - Higher Education Support Programme grant RS/HESP/1074/693/1995. A typology of "urban tissues" was developped. The "urban tissues", are middle-sized parts of the city, homogenous in their main quantitative characteristics - i.e. intensity of land use (COS, CES) and built-form height, defined in average number of stories (ANS, VyHL). The typologies were edevelopped fot both the collection of Slovak urban tissues identified by Faculty of Architecture in Bratislava research, and for a collection of Western Europe urban tissues.
Fulltext:  PDF
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Competitive analysis of selected catering facilities
Komrska, Jan ; Turnerová, Lenka (advisor) ; Průša, Přemysl (referee)
The objective of this bachelor thesis is to identify the most important catering providers for Žižkov premises of VŠE, analyze their activities and competitive position on the market and suggest possible opportunities for development of these entities. The theoretical part defines process of competitive analysis creation. The practical part is devoted to specific companies. In the closing is quantified comparison of these firms available.
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