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Is free will just an illusion?
KOHOUTOVÁ, Lucie
The question of free will is one of the oldest and most complex questions that one deals with. It is not only theoretical, because the consequences of our actions will affect not only our lives, but also the lives of other people. In my bachelor thesis I will rely on the definitions of free will and try to define the basic theories that work with it. What are the basic attitudes to free will and what are the arguments for its existence or non-existence? The main question will be whether we possess free will, whether we are able to make free choices, or whether the act of free will is predestined to us and our free will is then a mere illusion. I will try to present the arguments of some philosophers and show their view of free will and the related moral responsibility.
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JNK3 MAP-kinase localization in the brain of mice with Alzheimer's disease
Kohoutová, Lucie ; Rathouská, Jana (advisor) ; Herink, Josef (referee)
Charles University in Prague Faculty of Pharmacy in Hradec Králové Department of Biological and Medical Sciences Student: Lucie Kohoutová Supervisor: PharmDr. Jana Rathouská, Ph. D. Specialized supervisor: Dr. Maria Javier Ramirez Title of diploma thesis: JNK3 MAP-kinase localization in the brain of mice with Alzheimer's disease Background: The pathogenesis of Alzheimer's disease (AD) is incompletely understood. Discovery of c-Jun N-terminal kinases (JNKs), involved in regulation of gene expression, inflammation, cell proliferation and apoptosis, may contribute to better understanding of AD pathogenesis. JNK3 is a brain-specific JNK isoform involved in apoptosis of mammalian neurons. Precise localization of JNK3 in the brain still remains unclear. JNK3 silencing achieved by gene knockout had directly decreased beta amyloid (Aβ) levels in previous AD models in mice. We performed an immunohistological study to localize JNK3 gene in the mice brain in order to better focus future gene targeting therapy. Methods: A transgenic mice model of AD (Tg2576) has been used to study the expression of JNK3 by western blotting compared to the wild type. In the same brains localization of pJNK3 in astroglia (GFAP), microglia (Ox42), neurons (NeuN), senile plaques (Aβ) or pTau, using immunohistochemistry staining...
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Comparative analysis of council's magazine Praha 1 and the Pražan magazine
Kohoutová, Lucie ; Křeček, Jan (advisor) ; Kocourková, Lucie (referee)
Bachelor thesis "Comparative analysis of council's magazine Praha 1 and the Pražan magazine" deals with two magazines distributed to citizens of Prague 1st city district - the magazine published by city council and the magazine published by Věci veřejné, political party in opposition. The aim of this thesis is to compare preferred topics and image of townhall leadership and political opposition provided by the magazines in question, by means of the content analysis method. In the first part the terminology, related papers, law context and main problematic features of the council magazines are introduced, followed by description of serials (for which working title "opposition serials" is used) that stand and write against city councils. The following practical part first describes all involved institutions, local actors and the analyzed magazines including their production background. Content analysis of issues published between april 2009 and april 2010 is central to this thesis as it brings answers to the research questions: 1. which topics are the most salient in the analyzed magazines; 2. how often and in which context is portrayed the townhall leadership; 3. how often and in which context is portrayed the townhall opposition; 4. to which extent the magazines cover the same topics and write about each...
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Lyme borreliosis, clinical and laboratory diagnosis
Kohoutová, Lucie ; Jílek, Petr (advisor) ; Konečná, Klára (referee)
Author: Mgr. Lucie Kohoutová Title: Lyme borreliosis, clinical and laboratory diagnosis University: Charles University in Prague, Faculty of Pharmacy in Hradec Kralove Field of study: Pharmacy Background: We investigated the situation at laboratory and clinical diagnosis in the area of Havlíčkův Brod from 2011 to 2012. We focused on how the suggested laboratory methods were respected in the first half of our paper. Also one of our main interests was the number of patients with positive results of serology and liquor tests. The next aim was to point out how complex was the process of all tests evaluation to neuroborreliosis. The second part of our rigorous work was dedicated to the real state of diagnosis and therapy of the lyme disease in the area that belongs to Havlíčkův Brod's Hospital. Our aim was to verify if the recommended rules of diagnosing and therapy published by Czech Society of Infectology were respected. And of course if there's sufficient knowledge of the disease. Methods: The data were gained from the laboratory of immunology (Department of Collective Laboratories of Havlíčkův Brod's Hospital). Patients were judged in several categories: the result of particular exams (ELISA, westernblot, immunoblot) in time, the sex, the date of sampling (alternatively other samplings), the doctor...
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Characteristics of novel protein interactions of gamma-tubulin and their roles with microtubules and in cell division
Kohoutová, Lucie ; Binarová, Pavla (advisor) ; Kubelka, Michal (referee) ; Dráber, Pavel (referee)
Spatial and temporal regulation of microtubule nucleation and dynamics is required for formation of specific microtubular arrays that react to internal and external signals and change accordingly. Microtubules are nucleated from microtubule-organizing centres such as centrosomes in animal cells or spindle pole bodies in fungi. All higher plants lack centrosomes and thus present a model for study of acentrosomal cell division and microtubule nucleation and organization. γ-Tubulin is a conserved protein from tubulin superfamily with a central role in microtubule nucleation. It also regulates microtubule dynamics and organization including mitotic spindle positioning. Moreover, γ-tubulin functions in cell cycle regulation, checkpoints control, regulation of transcription, and coordination of late mitotic events. We aimed to characterize protein interactions of γ-tubulin and their functions in Arabidopsis. We identified Arabidopsis homologue of putative centrososomal protein RanBPM. Our data showed that AtRanBPM is a member of CTLH complexes. Our finding of CTLH complexes in plants confirmed their conservation in eukaryotic cells. We found that NITRILASE1 is a cell cycle regulator in Arabidopsis that is important for maintenance of genome stability and proper cell division. We studied a role of AtTPX2,...
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JNK3 MAP-kinase localization in the brain of mice with Alzheimer's disease
Kohoutová, Lucie ; Rathouská, Jana (advisor) ; Herink, Josef (referee)
Charles University in Prague Faculty of Pharmacy in Hradec Králové Department of Biological and Medical Sciences Student: Lucie Kohoutová Supervisor: PharmDr. Jana Rathouská, Ph. D. Specialized supervisor: Dr. Maria Javier Ramirez Title of diploma thesis: JNK3 MAP-kinase localization in the brain of mice with Alzheimer's disease Background: The pathogenesis of Alzheimer's disease (AD) is incompletely understood. Discovery of c-Jun N-terminal kinases (JNKs), involved in regulation of gene expression, inflammation, cell proliferation and apoptosis, may contribute to better understanding of AD pathogenesis. JNK3 is a brain-specific JNK isoform involved in apoptosis of mammalian neurons. Precise localization of JNK3 in the brain still remains unclear. JNK3 silencing achieved by gene knockout had directly decreased beta amyloid (Aβ) levels in previous AD models in mice. We performed an immunohistological study to localize JNK3 gene in the mice brain in order to better focus future gene targeting therapy. Methods: A transgenic mice model of AD (Tg2576) has been used to study the expression of JNK3 by western blotting compared to the wild type. In the same brains localization of pJNK3 in astroglia (GFAP), microglia (Ox42), neurons (NeuN), senile plaques (Aβ) or pTau, using immunohistochemistry staining...
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Zhe Scuk.cz Project: user-generated content from the perspective of quality control and creators' motivation. Case study
Kohoutová, Lucie ; Švelch, Jaroslav (advisor) ; Štechová, Markéta (referee)
This Master degree thesis deals with content quality regulation and motivation to participate in the Scuk.cz project - web server dedicated to reviews of restaurants and other culinary venues. The server represents a specific case of user-generated content (UGC) whose content comes from closed community of reviewers chosen among non-professional food lovers by the server's founder. Aim of this thesis is to describe this filtration and regulation process as well as the qualitative criteria applied to reviewers and reviews, both from the point of view of administrators and community members. It uses the theory of reputation systems as well as Carpentier's more dynamic concept of quality as the ever-changing result of democratic negotiations (in the context of community media, as Scuk.cz can be to a certain extent). Motivations for participation are explained with Bourdieu's concepts of social and cultural capital and their later derivations. Empirical part of the study is based on qualitative interviews with administrators and 11 community members and following thematical analysis. Interview data are completed with analysis of texts - Scuk guidelines, reviews and other community communication. We found out that the first access filter plays a key role in content regulation, creating like- minded...
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Lyme borreliosis
Kohoutová, Lucie ; Jílek, Petr (advisor) ; Křivčíková, Lucie (referee)
Author: Lucie Kohoutova Title: Lyme borreliosis (Diploma thesis) University: Charles University in Prague, Faculty of Pharmacy in Hradec Kralove Field of study: Pharmacy Background: We investigated the presence of Lyme disease in the area of Havlickuv Brod based on laboratory diagnostics of specific or non- specific antibodies in the blood serum and in the liquor. The aim was to define typical laboratory signs of illness and recommend racional methods in diagnosis of Lyme disease. Methods: We used the data from Havlickuv Brod hospital laboratory of immunology. Patients were dividend into the groups according to the results of basic tests (if there were immunoglobulines type M and type G in serum or liquor), sex, age, the day of blood collection, code of diagnosis, patient's doctor and the type of running tests (ELISA, westernblot). These groups were compared. Patients with at least one positive result in basic tests and with any of additional test at the same time were divided in compliance with relations amongst these types of tests. We tried to determine appropriate interval between taking samples during monitoring the dynamics of the disease by contribution- based method. Results: More threatned by Lyme borreliosis are older women and people over the age of fifty- five. The highest number of new...
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