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History of education in Zliv
KLÍMA, Martin
This bachelor thesis deals with the history of an elementary school in Zliv in 1889-1945. The introduction is focused on the history of the town and the history of education in Zliv. Due to the complexity of this topic, this thesis is focused only on a primary school in Zliv. The core of this thesis is comprised of two chapters, which are focused on the life at Zliv's primary school in the pre-war, interwar and war periods. This work describes the teaching staff and the headteachers who worked at school in the given period. Furthermore, it deals with the pupils and their school education in the given period. This work also includes important events which influenced the school's activity. The last topics, which this thesis deals with, are regular school events and celebrations. This bachelor thesis is mainly based on school chronicles and chronicles of the town.
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The role of inactive MTMR phosphatases in mammalian cells
Sixtová, Nikola ; Macůrková, Marie (advisor) ; Klíma, Martin (referee)
Variable composition of the cellular membranes influences many cellular events such as endosomal transport, autophagy or cellular signalling. The membrane identity is significantly determined by the specific distribution of phosphoinositide derivatives. These derivatives are specifically distributed among cellular membranes and they are tightly regulated by the interplay of corresponding lipid kinases and phosphatases. Myotubularins (MTMRs) form a family of phosphatases dephosphorylating phosphatidylinositol 3-phosphate and phosphatidylinositol 3,5-bisphosphate at the 3rd position of the inositol ring. Similarly to their substrates, MTMRs are involved in various cellular events such as endosomal transport or autophagy. Mutations in MTMR proteins lead to dysregulation of the cellular events and manifestation of severe pathologies. Among the most studied are two hereditary diseases, X- linked myotubular myopathy and Charcot-Marie-Tooth syndrome, caused by mutations in MTM1 and MTMR2 genes, respectively. One of the specific features of the MTMR family is the presence of catalytically inactive members. These members were found to regulate protein stability, activity and localization of their active partners. MTMR10 and MTMR12 are two inactive members of the MTMR family, directly interacting with the...
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Molecular and functional characterization of the death receptor 6
Klíma, Martin ; Anděra, Ladislav (advisor) ; Živný, Jan (referee) ; Kovář, Marek (referee)
Death receptor-6 (DR6/TNFRsf21/CD358) is a receptor from the TNFR superfamily that likely participates in the regulation of proliferation and differentiation of T- and B-lymphocytes and neural cells. The 655-amino acid human DR6 is a type I transmembrane protein containing four cysteine-rich domains in its extracelular part and a death domain followed by the CARD-like region in its cytoplasmic part. Overexpression of DR6 in some cell lines leads to apoptosis, and/or to activation of nuclear factor NF-κB and stress kinases of the JNK family. In the first part of our work we focused on molecular characterization of DR6, including the analysis of its posttranslational modifications. We found that DR6 is an extensively posttranslationally modified protein including S-palmitoylation and both N- and O-glycosylation. Six N-glycosylation and one S-palmitoylation sites were precisely mapped to appropriate asparagines and cysteine respectively. The juxtaposed linker region (between cystein-rich domains and the transmembrane part), which also contains Ser/Thr/Pro-rich region with clustered putative O-glycosylation sites, is required for the plasma membrane localization of DR6. N-glycosylation, but interestingly not S-palmitoylation, may play a role in targeting of DR6 into detergent-resistant...
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Molecular and functional characterization of the death receptor 6
Klíma, Martin
Death receptor-6 (DR6/TNFRsf21/CD358) is a receptor from the TNFR superfamily that likely participates in the regulation of proliferation and differentiation of T- and B-lymphocytes and neural cells. The 655-amino acid human DR6 is a type I transmembrane protein containing four cysteine-rich domains in its extracelular part and a death domain followed by the CARD-like region in its cytoplasmic part. Overexpression of DR6 in some cell lines leads to apoptosis, and/or to activation of nuclear factor NF-κB and stress kinases of the JNK family. In the first part of our work we focused on molecular characterization of DR6, including the analysis of its posttranslational modifications. We found that DR6 is an extensively posttranslationally modified protein including S-palmitoylation and both N- and O-glycosylation. Six N-glycosylation and one S-palmitoylation sites were precisely mapped to appropriate asparagines and cysteine respectively. The juxtaposed linker region (between cystein-rich domains and the transmembrane part), which also contains Ser/Thr/Pro-rich region with clustered putative O-glycosylation sites, is required for the plasma membrane localization of DR6. N-glycosylation, but interestingly not S-palmitoylation, may play a role in targeting of DR6 into detergent-resistant...
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The role of inactive MTMR phosphatases in mammalian cells
Sixtová, Nikola ; Macůrková, Marie (advisor) ; Klíma, Martin (referee)
Variable composition of the cellular membranes influences many cellular events such as endosomal transport, autophagy or cellular signalling. The membrane identity is significantly determined by the specific distribution of phosphoinositide derivatives. These derivatives are specifically distributed among cellular membranes and they are tightly regulated by the interplay of corresponding lipid kinases and phosphatases. Myotubularins (MTMRs) form a family of phosphatases dephosphorylating phosphatidylinositol 3-phosphate and phosphatidylinositol 3,5-bisphosphate at the 3rd position of the inositol ring. Similarly to their substrates, MTMRs are involved in various cellular events such as endosomal transport or autophagy. Mutations in MTMR proteins lead to dysregulation of the cellular events and manifestation of severe pathologies. Among the most studied are two hereditary diseases, X- linked myotubular myopathy and Charcot-Marie-Tooth syndrome, caused by mutations in MTM1 and MTMR2 genes, respectively. One of the specific features of the MTMR family is the presence of catalytically inactive members. These members were found to regulate protein stability, activity and localization of their active partners. MTMR10 and MTMR12 are two inactive members of the MTMR family, directly interacting with the...
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Combination of Evolutionary Algorithms and Constraint Programming for Scheduling
Štola, Miroslav ; Pilát, Martin (advisor) ; Klíma, Martin (referee)
Scheduling problems and constraint satisfaction problems are generally known to be extremely hard. This thesis proposes a new evolutionary al- gorithm approach to solve a constrained-based scheduling problem. In this approach, variable orderings are evolved. The variable ordering serves as a parameter for the constraint solver. Its purpose is to determine the order in which variables are labelled by the solver. Hence the evolving individuals may be encoded as permutations. Therefore, our approach can be applied to a wider range of constraint satisfaction problems. Methods for generating the initial population of individuals based on the analysis of the precedence constraints graph are proposed. New genetic operators are presented and successfully applied. Our approach succeeded in finding a range of diverse schedules with the optimal makespan. Furthermore, multi-objective opti- mization was successfully attempted with the NSGA-II. 1
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Process analysis of nonconforming products
KLÍMA, Martin
Main goal of thesis is process analysis of nonconforming products and implementation of the corrective actions which will improve current status. Theoretical part presents history of quality, modern methods in quality, process of nonconforming products, preventive methods used in car industry and also the costs of quality. Practical part presents process analysis of nonconforming products in concrete company and main focus is implementation of the corrective actions. Main point regarding the improvement is upgrading detection system. Upgrade was made on concrete product, which represented huge quality extracosts.
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Optimalizace tvorby rolí pomocí RBAC modelu
KLÍMA, Martin
The aim of the thesis is to develop algorithm which will be able to optimize roles using RBAC model. The intent of the theoretical part is to analyze RBAC model and present current options which are available for role optimization. The practical part deals with development of algorithm which allows to optimize roles based on defined criteria from user. This algorithm is implemented in programming language Java and builds on Role Process Optimization Model (ROPM). In the last part is showed on example set of data how this algorithm works, step by step, with explanation of each step. Result of this algorithm is new RBAC model defined by user criteria. In this thesis are also listed different approach in role optimization, possible future development and concept of mapping RBAC model to mathematical and data-mining techniques.
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Molecular and functional characterization of the death receptor 6
Klíma, Martin
Death receptor-6 (DR6/TNFRsf21/CD358) is a receptor from the TNFR superfamily that likely participates in the regulation of proliferation and differentiation of T- and B-lymphocytes and neural cells. The 655-amino acid human DR6 is a type I transmembrane protein containing four cysteine-rich domains in its extracelular part and a death domain followed by the CARD-like region in its cytoplasmic part. Overexpression of DR6 in some cell lines leads to apoptosis, and/or to activation of nuclear factor NF-κB and stress kinases of the JNK family. In the first part of our work we focused on molecular characterization of DR6, including the analysis of its posttranslational modifications. We found that DR6 is an extensively posttranslationally modified protein including S-palmitoylation and both N- and O-glycosylation. Six N-glycosylation and one S-palmitoylation sites were precisely mapped to appropriate asparagines and cysteine respectively. The juxtaposed linker region (between cystein-rich domains and the transmembrane part), which also contains Ser/Thr/Pro-rich region with clustered putative O-glycosylation sites, is required for the plasma membrane localization of DR6. N-glycosylation, but interestingly not S-palmitoylation, may play a role in targeting of DR6 into detergent-resistant...
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