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Enzyme activity and molecular characterization of fungi in decaying wood
KONAROVSKÁ, Kristýna
Decomposing wood is necessary part of forest ecosystem. Wood is robust and hardly decomposable material. Some fungi can use enzymes to degrade lingocellulose. This bachelor thesis is concerned with these fungi and enzymes. Goal of this work was to describe factors affecting composition of fungal community in deadwood. Two different kinds of trees - beech and fir had been sampled from trunks and branches and ergosterol content, fungal biomass, activity of extracellular enzymes and fungal community composition were determined. Main watched property has been different source of samples coming from tree trunks (T) or branches (B) and from Silver fir (FWD) or European beech (CWD) thus forming 4 categories FWD-T, FWD-B, CWD-T and CWD-B. Analysis confirmed higher potential activity of enzymes and higher pH in samples taken from branches specifically endocellulose, exocellulose, endoxylanase, N-acetylglukosaminidase a -glukosidase. Ergosterol marker confirmed higher biomass content in branches. Ascomycet or Basidiomycet fungi content was not dependent on type of wood or its size. Enzyme activity and fungi biomass with lower pH confirms higher fungi enzymes content in branches in comparison to trunks. Presence of fungi influences substrate thanks to active metabolism. Higher biomass values were linked to faster branches colonization and their decomposition.
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1 Konárovská, Kateřina
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