National Repository of Grey Literature 14 records found  1 - 10next  jump to record: Search took 0.01 seconds. 
Early Detection of Disease Progression in Patients with Myelodysplastic Syndromes.
Kaisrlíková, Monika ; Beličková, Monika (advisor) ; Kalinová, Markéta (referee) ; Zemanová, Zuzana (referee)
Myelodysplastic syndromes (MDS) are a heterogeneous group of clonal hematopoietic disorders with a risk of transformation into acute myeloid leukemia (AML). The International Prognostic Scoring Systems integrate clinical data and cytogenetics to determine the risk of AML transformation for individual patients. Precise risk assessment is crucial for treatment decision- making. The aim of this thesis was to identify molecular markers for the early detection of disease progression in MDS patients. Using cDNA microarrays and next-generation sequencing, we targeted long noncoding RNAs (lncRNAs) and recurrently mutated genes in bone marrow cells. In addition, we focused on the identification of pathways related to the progression of MDS and understanding how the identified biomarkers participate. In the transcriptome study, we identify 4 candidate lncRNAs that may serve as prognostic biomarkers of the adverse course of MDS: H19, WT1-AS, TCL6, and LEF1-AS. Using various statistical approaches, we determined the level of H19 to be a strong independent prognostic marker. Furthermore, our data showed that disruption of transcriptional coregulation of the imprinting locus H19/IGF2 and miR-675, which directly regulates H19 and plays a role in tumorigenesis, accompanies disease progression. In the genomic study...
Patient-derived xenograft models of human lymphomas for experimental immunotherapy
Jakša, Radek ; Klener, Pavel (advisor) ; Škarda, Jozef (referee) ; Kalinová, Markéta (referee)
Non-Hodgkin lymphomas (NHL) represent the most common hematologic malignancies. Patient-derived xenografts (PDX) are used for various aspects of translational research including preclinical in vivo validation of experimental treatment approaches. While it was repeatedly demonstrated that PDX keep majority of somatic mutations with the primary lymphoma samples, from which they were derived, the composition of PDX tumor microenvironment (TME) has not been extensively studied. We derived 15 PDX models from patients with various subtypes of aggressive lymphomas. We implemented complex genetic and immunohistochemical analysis of the established PDX models head-to-head with the patient's primary lymphoma cells, from which the PDXs were derived. We clearly confirmed that the established PDX cells shared majority of somatic mutations with the patient's primary cells, from which they were derived. Thus, from the genetic perspective the PDX models represent relevant tools for the study of lymphoma biology. Immunohistochemistry analysis of selected antigens revealed some differences between the PDXs and patients' primary cells. Importantly, the analysis demonstrated complete loss of non-malignant cellular components of the tumor microenvironment frequently observed in lymphoma infiltrated lymph nodes,...
The possibilities of individualisation, differentiation and personalization in teaching and learning strategies
Kalinová, Markéta ; Mojžíšová, Jarmila (advisor) ; Nováková, Daniela (referee)
Markéta Kalinová Abstract The thesis titled The possibilities of individualisation, differentiation and personalization in teaching and learning strategies deals with teaching and learning strategies, forms and methods suitable for teacher's work in a mixed ability classroom. Theoretical basis results from the presupposition that every human being and naturally also every pupil is different and has their own educational needs. The thesis tries to find opportunities how to fulfil these needs within the framework of collective schooling. It also concerns the development of differentiated teaching in the history of education. The subject matter is also described in connection with the recent Czech educational reform. The empirical research describes a situation in one Czech basic school. The aim was to find out elements of differentiated instruction. Key words: differentiation, individualisation, pupil's learning style, individual student progress, mastery learning, success for all learners
Quantitative detection of the expression of gene WT1: prognostic significance and monitoring of residual disease in child hematological diseases
Kalinová, Markéta ; Trka, Jan (advisor) ; Zemanová, Zuzana (referee) ; Haškovec, Cedrick (referee) ; Mareš, Jaroslav (referee)
Quantitative detection of the expression of gene WT1: prognostic significance and monitoring of residual disease in child hematological diseases Powered by TCPDF (www.tcpdf.org)
The Role of Antiangiogenic Therapy in Mantle Cell Lymphoma
Kovaříková, Petra ; Klener, Pavel (advisor) ; Kalinová, Markéta (referee) ; Boudová, Ludmila (referee)
Mantle cell lymphoma (MCL) is a subtype of B-non-Hodgkin's lymphoma, characterized by often relapses. Despite an Ibrutinib (a Bruton's kinase inhibitor) implementation into salvage therapy, these patients often relapse with biologically highly aggressive disease and very poor prognosis. An increased activation of alternative metabolic pathways was described as one of ibrutinib-resistance mechanisms. Some of these pathways have also significant proangiogenic activity (e.g. PI3K-AKT-mTOR). In presented study, we established and standardized a real-time ultrasound and photoacoustic imaging of neovascularization and tissue oxygenation of subcutaneous MCL tumors in mice. Ultrasound and photoacoustic imaging is a fast, non-invasive method for angiogenesis evaluation in subcutaneous tumors with huge preclinical potential. Using MCL mice models, we also demonstrated the importance of CD31/PECAM-1 expression for engraftment, growth and spread of MCL cells in vivo. The level of CD31 expression in primary MCL cell (obtained directly from MCL patients) positively correlates with extent of extranodal involvement. CD31 facilitates survival and regulates extranodal spread of mantle cell lymphoma. We found that increased VEGFA expression causes not only increased microvessel density due to higher sprouting...
Molecular genetic aspects of neurodegenerative diseases
Parobková, Eva ; Matěj, Radoslav (advisor) ; Kalinová, Markéta (referee) ; Menšíková, Kateřina (referee)
Genetics plays a crucial role in translational research, which ultimately aims to develop new therapies that modify neurodegenerative disorders. We anticipate that individual genetic profiling will become increasingly important in a clinical context with implications for patient care in line with the proposed ideal of personalized medicine. Although whole exome sequencing (WES) is now widely used in gene identification studies, there is no doubt that whole genome sequencing (WGS) will soon replace WES as the standard method for gene discovery. Hopefully, in the near future, comparable initiatives will be developed to generate global, fully genomic, publicly available data for various forms of neurodegenerative diseases such as FTD. In the first part of this work we focused on a group of patients diagnosed with "pure" sCJD and sCJD and PART or early phase AD and sCJD with more advanced AD. We compared and analyzed a new generation sequencing (NGS) method of 15 genes that may be involved in the development of the disease. We identified a p.E318G polymorphism in the PSEN1 gene, which was found in three patients (10.3%), one of whom had pure sCJD and two were sCJD + AD. No relevant variants were observed for the other AD APP and PSEN2 genes. In PRNP, insertion of p.P84_Q91Q was detected in one patient...
A healthy lifestyle and a family with a child with disabilities
KALINOVÁ, Markéta
This thesis deals with the healthy lifestyle of parents of children with disabilities. The aim of the work, which is divided into four parts, is to analyze specifically set thematic parts related to the lives of families of children with disabilities and to create a relatively comprehensive synthesis mapping and reflecting on the given issue programmatically from various perspectives. The thesis also points out neglected connections in the life of parents of children with disabilities and stereotypes and prejudices of society.
Immunoglobulin genes rearrangement and minimal residual disease monitoring in B-lymphoproliferative disease.
Lokvenc, Milan ; Kalinová, Markéta (advisor) ; Krulová, Magdaléna (referee)
Malignant lymphomas are tumors arising by clonal proliferation of lymphocytes stopped at a specific stage of differentiation. All tumor cells arising from the original clone thus share the same characteristics and that can be used in their detection. Finding a suitable molecular marker of tumor cells is an essential step not only to disease diagnosis, but also for monitoring of minimal residual disease. Minimal residual disease is defined as the subclinical disease level, which malignant cells are not detectable for conventional cytological methods during the therapy. These residual cells can cause relapse. The main goals of the diploma thesis are a detection and analysis of immunoglobulin genes rearrangement and chromosomal translocation t(11; 14) in the MTC region, and a development and optimization of RQ-PCR system for detection of minimal residual disease. Quantification of clonal rearrangement or chromosomal translocation allows the detection of minimal residual disease level in patients with malignant lymphomas. Clonal immunoglobulin genes rearrangement or characteristic chromosomal translocation were analyzed in 19 patients with malignant lymphomas. There were analyzed individual gene segments, N-region and combination variability in immunoglobulin genes rearrangement. There was developed...
The possibilities of individualisation, differentiation and personalization in teaching and learning strategies
Kalinová, Markéta ; Mojžíšová, Jarmila (advisor) ; Nováková, Daniela (referee)
Markéta Kalinová Abstract The thesis titled The possibilities of individualisation, differentiation and personalization in teaching and learning strategies deals with teaching and learning strategies, forms and methods suitable for teacher's work in a mixed ability classroom. Theoretical basis results from the presupposition that every human being and naturally also every pupil is different and has their own educational needs. The thesis tries to find opportunities how to fulfil these needs within the framework of collective schooling. It also concerns the development of differentiated teaching in the history of education. The subject matter is also described in connection with the recent Czech educational reform. The empirical research describes a situation in one Czech basic school. The aim was to find out elements of differentiated instruction. Key words: differentiation, individualisation, pupil's learning style, individual student progress, mastery learning, success for all learners

National Repository of Grey Literature : 14 records found   1 - 10next  jump to record:
See also: similar author names
4 KALINOVÁ, Michaela
1 Kalinová, Mariana
5 Kalinová, Marie
8 Kalinová, Markéta
4 Kalinová, Martina
4 Kalinová, Michaela
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