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DESIGN OF FIBER-OPTIC BIOSENSOR WITH NEAR-INFRARED SPECTRAL ANALYSIS
Křepelka, Pavel ; Jakubec,, Martin (referee) ; Skládal, Petr (referee) ; Mikulka, Jan (advisor)
This thesis deals with a measurement and interpretation of NIR spectra of bacterial cells and design of biosensor using this analytical technique. In the first chapter, there is introduction of current state of knowledge in the field of NIR spectroscopy in microbiology and technology of fiber optic biosensors. The summary of this chapter shows that NIR is a suitable technique for direct molecular analysis of bacteria, but it suffers from low sensitivity and insufficient interpretation of bacterial spectra. In the next part of the thesis, there is a theoretical background of spectral analysis techniques and technology of fiber optic sensors. In the practical part of this work, there is suggested the elimination of disadvantages of NIR spectroscopy in microbiology by a series of experiments used for interpretation of NIR spectra of bacteria and design of fiber optic sensor to increase sensitivity of this technique. In this work, spectral regions important for the identification of bacterial strains were determined and partially interpreted and the sensor for bacterial analysis capable of classifying strains based on 105 captured cells was designed. Therefore, the objectives of this work were fulfilled.
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Ultrasonic Anemometer
Křepelka, Pavel ; Fialka, Jiří (referee) ; Beneš, Petr (advisor)
In the first section of this document, there are described principles of the wind measuring. Anemometers can be divides by physical principles to mechanical (propeller or cup), ultrasonic anemometers and thermoanemometers. This paper is focused to ultrasonic anemometers. In the next part, there are described principles of sound propagation in the atmosphere. There are studies of measurement theory and comparing of different approaches. Completed solutions are presented in patent exploration. Afterwards, there are introduction of market research. Important parameters of ultrasonic anemometers are highlighted. Main aim of work is designing prototype of ultrasonic anemometer. Software, hardware and construct of experimental anemometer were designed. Created anemometer was calibrated in aerodynamics tunnel and results were compared with simulation output.
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Pulsed driver for laser diodes
Pernica, Lukáš ; Křepelka, Pavel (referee) ; Drexler, Petr (advisor)
This semestral thesis describes creation of laser radiation in the semiconductor material, the structure of the laser diode and the possibility of using power laser diodes in pulsed mode with high switching frequencies. In the thesis are described methods of casing of power laser diodes and their cooling possibilities and solutions to their switching, evaluating the pros and cons of these solutions and select the most suitable one for the best completion of all requirements.
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Indoor Robot - Control Neural Network
Křepelka, Pavel ; Kopečný, Lukáš (referee) ; Žalud, Luděk (advisor)
In this document, I describe possibilities of mobile robot navigation. This problems are solving many different ways, but there isn’t satisfactorily result to this day. You find there describe of deterministic algorithms, this algorithms can be used for simply actions like obstacle avoiding or travel in corridor. For global navigation this algorithms fails. In next part of document is theory of artificial neural nets (perceptron, multi layer neural nets, self organization map) and using them in mobile robots. Own navigation algorithms was tested on constructed mobile robot or simulated in SW described in chapter 6. Design own control algorithms is based on neural net (Kohonen net). Designed algorithms can be used for one-point navigation or complex global navigation. In document, there is comparing of various ways to navigation, their advantages and disadvantages. Goal of this document is find effective algorithm for navigation and artificial intelligence appears to be the right solution.
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Audio amplifier for detecting wood-destroying pests
Jaroš, Ondřej ; Křepelka, Pavel (referee) ; Friedl, Martin (advisor)
This bachelor´s thesis is focused on design of detector of wood-destroying pests, which is solved by using an acoustic sound source with piezo electric element as a sensor. The acquired signal is step by step amplified to a level when we can observe the audio spectrum using a headphone. The realized audio amplifier is complemented supplemented by an active analog filter for the ability to listen to a narrower frequency spectrum. The next feature of the detector is that it displays the frequency spectrum on an LCD display ranging from 0 Hz to 10 kHz. The spectrum of the signal is acquired by using DFT transformation. Analysis of the device is firstly described from the theoretical part through the realized design. At the end of document the results are summarized.
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DESIGN OF FIBER-OPTIC BIOSENSOR WITH NEAR-INFRARED SPECTRAL ANALYSIS
Křepelka, Pavel ; Jakubec,, Martin (referee) ; Skládal, Petr (referee) ; Mikulka, Jan (advisor)
This thesis deals with a measurement and interpretation of NIR spectra of bacterial cells and design of biosensor using this analytical technique. In the first chapter, there is introduction of current state of knowledge in the field of NIR spectroscopy in microbiology and technology of fiber optic biosensors. The summary of this chapter shows that NIR is a suitable technique for direct molecular analysis of bacteria, but it suffers from low sensitivity and insufficient interpretation of bacterial spectra. In the next part of the thesis, there is a theoretical background of spectral analysis techniques and technology of fiber optic sensors. In the practical part of this work, there is suggested the elimination of disadvantages of NIR spectroscopy in microbiology by a series of experiments used for interpretation of NIR spectra of bacteria and design of fiber optic sensor to increase sensitivity of this technique. In this work, spectral regions important for the identification of bacterial strains were determined and partially interpreted and the sensor for bacterial analysis capable of classifying strains based on 105 captured cells was designed. Therefore, the objectives of this work were fulfilled.
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|
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Pulsed driver for laser diodes
Pernica, Lukáš ; Křepelka, Pavel (referee) ; Drexler, Petr (advisor)
This semestral thesis describes creation of laser radiation in the semiconductor material, the structure of the laser diode and the possibility of using power laser diodes in pulsed mode with high switching frequencies. In the thesis are described methods of casing of power laser diodes and their cooling possibilities and solutions to their switching, evaluating the pros and cons of these solutions and select the most suitable one for the best completion of all requirements.
|
|
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Audio amplifier for detecting wood-destroying pests
Jaroš, Ondřej ; Křepelka, Pavel (referee) ; Friedl, Martin (advisor)
This bachelor´s thesis is focused on design of detector of wood-destroying pests, which is solved by using an acoustic sound source with piezo electric element as a sensor. The acquired signal is step by step amplified to a level when we can observe the audio spectrum using a headphone. The realized audio amplifier is complemented supplemented by an active analog filter for the ability to listen to a narrower frequency spectrum. The next feature of the detector is that it displays the frequency spectrum on an LCD display ranging from 0 Hz to 10 kHz. The spectrum of the signal is acquired by using DFT transformation. Analysis of the device is firstly described from the theoretical part through the realized design. At the end of document the results are summarized.
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Indoor Robot - Control Neural Network
Křepelka, Pavel ; Kopečný, Lukáš (referee) ; Žalud, Luděk (advisor)
In this document, I describe possibilities of mobile robot navigation. This problems are solving many different ways, but there isn’t satisfactorily result to this day. You find there describe of deterministic algorithms, this algorithms can be used for simply actions like obstacle avoiding or travel in corridor. For global navigation this algorithms fails. In next part of document is theory of artificial neural nets (perceptron, multi layer neural nets, self organization map) and using them in mobile robots. Own navigation algorithms was tested on constructed mobile robot or simulated in SW described in chapter 6. Design own control algorithms is based on neural net (Kohonen net). Designed algorithms can be used for one-point navigation or complex global navigation. In document, there is comparing of various ways to navigation, their advantages and disadvantages. Goal of this document is find effective algorithm for navigation and artificial intelligence appears to be the right solution.
|
|
|
Ultrasonic Anemometer
Křepelka, Pavel ; Fialka, Jiří (referee) ; Beneš, Petr (advisor)
In the first section of this document, there are described principles of the wind measuring. Anemometers can be divides by physical principles to mechanical (propeller or cup), ultrasonic anemometers and thermoanemometers. This paper is focused to ultrasonic anemometers. In the next part, there are described principles of sound propagation in the atmosphere. There are studies of measurement theory and comparing of different approaches. Completed solutions are presented in patent exploration. Afterwards, there are introduction of market research. Important parameters of ultrasonic anemometers are highlighted. Main aim of work is designing prototype of ultrasonic anemometer. Software, hardware and construct of experimental anemometer were designed. Created anemometer was calibrated in aerodynamics tunnel and results were compared with simulation output.
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