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The development of the microregion on topographical and thematical maps
Jílková, Jana ; Pospíšil, Lubomil (referee) ; Plánka, Ladislav (advisor)
This master thesis deals development of geographical names, cartographic symbols and on thematically maps documents changes of land use in microregion Lomnicko. Basis for its elaboration were analog and digital topographical and thematically maps from various sources and different time of periods. A part of the thesis are characteristics of used maps documents and conditions of their obtaining. Development of names and cartographic symbols is documented by tables and charts.
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Influencing of molecular mass of hyaluronic acid by fermentation process
Wikarská, Monika ; Jílková, Jana (referee) ; Velebný, Vladimír (advisor)
The biological function of hyaluronic acid (HA) – the polysaccharide, which consists of repeating units of N-actylglucosamine and glucuronic acid – is extremely broad and often contradictory, depending on its molecular weight (MW). The bacterial production provides HA of high molecular weight and purity. The objective of this thesis is to control the molecular weight of produced HA by the addition of hyaluronidases during the process of fermentation, resulting in reduction of viscosity of the medium followed by increase of yield. Hyaluronic acid was prepared by batch fermentation of the microorganism Streptococcus equi subsp. zooepidemicus. During the process of fermentation, the enzyme hyaluronate 4-glycanohydrolase (BTH) at various concentrations and different times of cultivation was added to bioreactors. The HA yield, the molecular weight and the polydispersity by the SEC-MALLS were monitored, together with the residual glucose concentration and the dynamic viscosity of the production medium. The formation of low and medium molecular weight HA was achieved. The addition of 10 U (BTH) / g (HA) had no significant effect on the molecular weight and therefore the HA function, but the corresponding drop of viscosity was noteworthy for the simplification of the product purification. Potential increase in HA yield was not confirmed because of the very low concentration of residual glucose and the method of precipitating low and medium MW HA.
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Princip nákladové přiměřenosti v právních předpisech EU v oblasti životního prostředí: ponaučení z implementace ochrany vod a ovzduší
Brabec, Jan ; Jílková, Jiřina (advisor) ; Knápek, Jaroslav (referee) ; Slavíková, Lenka (referee)
Environmental legislation in the European Union recognizes the principle of proportionality as a tool that helps identify cases that require spending of disproportionate resources to achieve environmental targets. If economic costs of achieving a certain target are significantly higher than associated benefits, an exemption may be granted to prevent wasting resources on measures with only a negligible effect on the environment. The principle is well established in water protection by means of the Water Framework Directive (2000/60/EC) and in air protection through the Industrial Emissions Directive (2010/75/EU). In theory, such setting should ensure more efficient and robust water and air protection. However, with no unified methodology and a high level of discretion given to individual member states, the process of assessing proportionality has become very heterogenous. As a result, methodologies used across Europe often differ from each other significantly, possibly distorting the original idea of equal treatment of European water bodies and sources of air pollution. The goal of this thesis is to determine whether the use of the proportionality principle in water and air protection contributes to more efficient environmental protection in the EU and whether the principle is used consistently in...
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Aplikace nástrojů hospodářské politiky v oblasti managementu přírodních zdrojů se zvláštním zaměřením na koncept ekosystémových služeb
Louda, Jiří ; Jílková, Jiřina (advisor) ; Geuss, Erik (referee) ; van Koten, Silvester (referee)
The dissertation deals with application of economic approaches and instruments in natural resource management. The paper puts a special emphasis on the developing concept of ecosystem services (mainly abroad) and its application in the Czech Republic's context. The objective of the thesis is to cultivate academic discussion of the importance of methods employed in environmental economics for application of economic policy tools in natural resource management in the Czech Republic's context and, among other things, to clarify whether (and if so, how) the concept of ecosystem services may contribute to the formulation of these tools. The paper also discusses possible limitations of using purely quantitative econometric methods in environmental policy. The dissertation is designed as a cumulative paper. The results of five scholarly articles presented document that application of economic approaches (such as valuation of environmental goods and services) may contribute to efficient meeting of environmental policy goals and that the ecosystem service concept brings new capabilities for application of economic theories in practical natural resource management. One of the new instruments grounded in this concept is the payments for ecosystem services, increasingly implemented abroad. However, the research results also point out the fact that isolated application of outcomes from quantitative environmental goods and services/ecosystems assessment methods has its limitations and may lead to design of measures and tools that will not have the desired effect. The recommendation is therefore to combine outcomes from quantitative methods with qualitative approaches (such as institutional analysis or stakeholder analysis).
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Influencing of molecular mass of hyaluronic acid by fermentation process
Wikarská, Monika ; Jílková, Jana (referee) ; Velebný, Vladimír (advisor)
The biological function of hyaluronic acid (HA) – the polysaccharide, which consists of repeating units of N-actylglucosamine and glucuronic acid – is extremely broad and often contradictory, depending on its molecular weight (MW). The bacterial production provides HA of high molecular weight and purity. The objective of this thesis is to control the molecular weight of produced HA by the addition of hyaluronidases during the process of fermentation, resulting in reduction of viscosity of the medium followed by increase of yield. Hyaluronic acid was prepared by batch fermentation of the microorganism Streptococcus equi subsp. zooepidemicus. During the process of fermentation, the enzyme hyaluronate 4-glycanohydrolase (BTH) at various concentrations and different times of cultivation was added to bioreactors. The HA yield, the molecular weight and the polydispersity by the SEC-MALLS were monitored, together with the residual glucose concentration and the dynamic viscosity of the production medium. The formation of low and medium molecular weight HA was achieved. The addition of 10 U (BTH) / g (HA) had no significant effect on the molecular weight and therefore the HA function, but the corresponding drop of viscosity was noteworthy for the simplification of the product purification. Potential increase in HA yield was not confirmed because of the very low concentration of residual glucose and the method of precipitating low and medium MW HA.
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Determinants of Foreign Language Learning
Jílková, Jana ; Prokop, Jiří (advisor) ; Betáková, Lucie (referee) ; Smetáček, Vladimír (referee)
The purpose of this doctoral thesis is to analyse chosen factors involved in foreign language acquisition, compare three types of assessment strategies of foreign language knowledge and skills to look for correlations between students' outcomes, their school and out-of-school background and time factors. In this mixed research study the data collected from documents, questionnaires and semi-structured interviews were analysed. State language examination candidates and upper- secondary students who were to take the new state school-leaving examination are involved in the study. The results show statistically significant relationship between the formal assessment by the teachers, through standardised examinations and by self-evaluation and factors as age when the learners started the foreign language learning, learning materials used and teachers' background. These findings may be used to provide feedback for strategic changes in study achievements to students, teachers and examiners.
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