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New approaches for voltammetric determination of tumour biomarkers and antidotes in urine
Hrdlička, Vojtěch ; Barek, Jiří (advisor) ; Novotný, Ladislav (referee) ; Skopalová, Jana (referee)
This Ph. D. thesis presents new methods for the determination of selected clinically relevant electrochemically active compounds. The first part deals with development of determination of tumour biomarkers homovanillic acid (HVA) and vanillylmandelic acid (VMA) in human urine with the use of hollow-fibre based liquid-phase microextraction (HF-LPME) and differential pulse voltammetry (DPV) at cathodically pre-treated boron doped diamond electrode (BDDE). Optimum conditions for HF-LPME-DPV of HVA and VMA were as follows: butyl benzoate as supported liquid membrane formed on porous polypropylene hollow-fibre, 0.1 mol L−1 HCl as donor phase and 30 min extraction time. Optimum acceptor phases were 0.1 mol L−1 phosphate buffer of pH 6 with ionic strength set to 0.55 mol L−1 for HVAand 0.1 mol L−1 NaOH for VMA, respectively. HF-LPME-DPV concentration dependencies for HVA and VMAwere linear in the range from 0.4 to 100 µmol L−1 and 0.5 to 100 µmol L−1 . Limits of quantification (LOQ)/detection (LOD) were 1.2/0.4 µmol L−1 for HVA and 1.7/0.5 µmol L−1 for VMA, respectively. The applicability of the developed methods was verified by analysis of human urine. In the second part, voltammetric behaviour of heavy metal poisoning antidote 2,3- dimercapto-1-propane-sulfonic acid (DMPS) was investigated with the use...
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The Waldsassen Abbey between Kingdom of Bohemia and Holy Roman Empire. The Political, Economic and Social Development of the Abbey and Its Manor in the 15th Century
Hrdlička, Vojtěch ; Zdichynec, Jan (advisor) ; Charvátová, Kateřina (referee)
The goal of this thesis was to analyse the development of abbey in Waldsassen since the end of 14th century to the finish of George's of Podiebrad reign. Author struggled to describe on the basis of analysis of above all unpublished monastic sources, which are deposited in State archive in Amberg, various aspects of life in Waldsassen convent. Apart from the economic base and its changes, which are well covered by amount of sources, also social a political relations to secular power in the Holy Roman Empire - roman rulers and princes are studied. Then the basis deals with contacts of monastery with the Crown of Bohemia and its aristocracy and also with the position of Waldsassen in the Order and its cooperation with other Cistercian monasteries. Matters dealt with the papal throne are not forgetten, too. Detailed analysis was dedicated to parish network and the land register of Waldsassen, which presents the valuable source for former economic relationships and toponyms of "Stiftland" and Upper Palatine. The fourth chapter is also comprehensive, describing Waldsassen during the Hussite wars and eliminates some myths about this period in the region. Closing parts of this thesis are dedicated to the renewal of abbey domain and to the effort, which was invested mutually to maintain good relations...
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Hollow-fibre Based Microextraction and Voltammetric Determination of Vanillylmandelic Acid
Hrdlička, Vojtěch ; Barek, J. ; Navrátil, Tomáš
New method of liquid/liquid/liquid hollow fibre based microextraction of vanillylmandelic acid coupled with voltammetric determination is being developed. Selected liquid membranes based on alcohols, aliphatic hydrocarbons and esters were tested. Highest enrichment factor of 2.52 after 30 minutes of extraction was obtained using propyl benzoate, RSD = 18.2 % (n=5). Mechanically and cathodically pretreated (-1000 mV vs. Ag/AgCl/3M KCl, 15 s) boron doped diamond electrode in a miniaturized arrangement was used for DPV determination. LOD = 1.2 micromol/l, RSD = 5.9% (n=10) for a 10 microl extract.
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The Beginnings of the Independent Czech Provincialate of the Augustinian Eremites Order in the Saint Thomas Monastery from 1604 to cca 1650
Hrdlička, Vojtěch ; Hojda, Zdeněk (advisor) ; Zdichynec, Jan (referee)
Annotation/Abstract: This thesis has aim to express and define circumstances the establishment of the independent Bohemia province of the order of St. Augustin in 1604 and its development to the end of Thirty Years War. The main emphasis will be put in the constructing of adminstration of new province and in personality of Jan Svitavský z Bochova, the first adminstrator. In view, that residence of the provincialate was the St. Thomas convent in Malá Strana, is necessary to point out the importance of this order convent and describe its dual role: first like seat of administrator, the second like the most important convent among other Augustinian convents of Land of the Crown of Bohemia with respect for its political and cultural significance. The author concentrates on relationships between administrator and convent of St. Thomas in Prague, and particular convents with their priors. Among important aims of thesis will be the discovery, how large autonomy of Augustinian convents was in Czech province and what their relation was with administrator, then detect relationships of administrator to general prior in Rome and papal curia. The establishment of independent Czech province in 1604 has of course connections with first re-Catholization attemps and with the position of St. Thomas convent in complicated...
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The History of Waldsassen Abbey since its Foundation to 1420 in Relation to Rulers of Bohemia and to the Cistercian Order
Hrdlička, Vojtěch ; Zdichynec, Jan (advisor) ; Bobková, Lenka (referee)
Annotation/Abstract: This bachelor thesis deals with the history and the development of cistercian abbey in Waldsassen since its origin in 1133 to 1420. This year is upper time limit of this thesis. This thesis should cover the reasons of foundation of this monastery, its imfluence, power in the Cheb region and his financial and economic relations and also changes inside monastic community. Important are social relations among particular members of the convent, especially part of the abbot like director of the monastery is pointed out. Author attempted to show connections of abbey in politics and participation off its members in the forming of history of Central Europe. Although Waldsassen was active in the Holy Roman Empire and developed its relations with the territories of Empire (Bavaria, Upper Palatinate, Egerland), it had the connections to the lands of the Crown of Bohemia and their rulers as well. It is the reason, why the most extensive chapter of this thesis is the fourth one about own history of abbey, the fifth one describes the relations of monastery with the czech sovereigns, but the connections of rulers of lands of the Crown of Bohemia were usually more feeble than these with the rulers of Roman empire.
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Electrochemical studies of the analytical utilization of self-assembled monolayers of calix[4]arene on the surface of polycrystalline gold electrode
Hrdlička, Vojtěch ; Navrátil, Tomáš (advisor) ; Ludvík, Jiří (referee)
This MSc. Thesis deals with electrochemical studies of the analytical utilization of self-assembled monolayers of thiolated calix[4]arene (C4A) and/or undecanthiole (C11SH) on the surface of a polycrystalline gold electrode. Hydroquinone, nitrobenzene, 2-nitrophenole and 4-nitrophenole have been used as tested model compounds. The real surface area of the electrode was determined using these two methods: From the charge of desorption peak of α-gold oxides (5.72±0.48 mm2 ) and using the Randles- Ševčík equation. (6.14±0.59 mm2 ). The preparation of the electrode consisted of polishing on alumina, immersing into piranha solution and cyclic voltammetry. The area of electrode surface was well reproducible. Stability of thiol monolayer in the area of negative potentials was tested. The C11SH layer is very stable; the desorption peak was recorded in basic solutions only (at −1.3 V vs. 3M Ag/AgCl). The position of the desorption peak of C4A is more pH dependent and it was recorded at −1.08 V at pH 13. The properties of the electrode modified by C11SH and or C4A were investigated using the model compound hydroquinone. It is assumed that the oxidation of the hydroquinone does not take place inside the C4A cavity; hence the hydroquinone molecule is too big to pass through the cavity. C11SH and C4 + C11SH act...
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Voltammetric Determination of 5-Nitroindazole Using Hanging Mercury Drop Electrode Different Electrode and Polished Silver Solid Amalgam Electrode
Hrdlička, Vojtěch ; Navrátil, Tomáš (advisor) ; Zima, Jiří (referee)
The presented Bachelor Thesis deals with a study of electrochemical behavior of 5-nitroindazole (5-NI) with the search of optimal conditions for its determination using tech- niques of DC voltammetry (DCV) and differential pulse voltammetry (DPV) at hanging mer- cury drop electrode (HMDE) and polished silver solid amalgam electrode (p-AgSAE), and with the comparison of reached limits of quantification (LQ ). The optimal conditions found for determination of 5-NI in deionized water using DPV at HDME were as follows: Britton-Robinson (BR) buffer of pH 8.0 and inital potential of +100 mV. The calibration dependences were measured in the concentration range from 1·10-7 to 1·10-5 mol·dm-3 with the LQ of 1.8·10-7 mol·dm-3 . Moreover, the posibility of using adsorbtion stirring voltametry (AdSV) for determination of 5-NI in BR buffer of pH 8 was investigated. The method was ineffective due to rising base- line. The mechanism of electrochemical reduction and oxidation in BR buffer of pH 8 at HMDE was investigated. It was found that the reduction of 5-NI was controlled by diffusion and the following oxidation was controlled by adsorbtion. For determination of 5-NI in deionized water using DVP at p-AgSAE, optimal conditions seemed to be as follows: BR buffer of pH 8.0 and initial potential of +100 mV....
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