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On-line SPE HPLC method optimization for determination of patulin mycotoxin in apple drinks
Holznerová, Anežka ; Šatínský, Dalibor (advisor) ; Solich, Petr (referee)
Charles University in Prague Faculty of Pharmacy in Hradec Králové Department of Analytical Chemistry Candidate: Bc. Anežka Holznerová Supervisor: Doc. RNDr. Dalibor Šatínský, Ph.D. Title of Diploma Thesis: On-line SPE HPLC method optimization for determination of patulin mycotoxin in apple drinks The issue of food contamination with mycotoxins is a serious problem worldwide. These substances are highly toxic to humans and chronic effects on the human organism in very low doses may cause long-term medical complications. In our work we focused on one of the most common dietary mycotoxins - patulin, which is found in apples and related products (especially apple juices). High performance liquid chromatography (HPLC) coupled with on- line solid phase extraction (SPE) using a column switching technique for sample treatment was developed for determination of mycotoxin patulin in apple drinks and juices. A volume of 250 µl of juice sample was injected directly into the on-line SPE-HPLC system. After injection of the sample the extraction of patulin from juice matrix was carried out on SPE precolumn. SPE precolumn 25 x 3 mm was filled with Supel MIP Patulin sorbent, which is a specific "molecularly imprinted polymer" (MIP) designated for the selective extraction of patulin from an apple matrix. As the...
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On-line SPE HPLC method optimization for determination of patulin mycotoxin in apple drinks
Holznerová, Anežka ; Šatínský, Dalibor (advisor) ; Solich, Petr (referee)
Charles University in Prague Faculty of Pharmacy in Hradec Králové Department of Analytical Chemistry Candidate: Bc. Anežka Holznerová Supervisor: Doc. RNDr. Dalibor Šatínský, Ph.D. Title of Diploma Thesis: On-line SPE HPLC method optimization for determination of patulin mycotoxin in apple drinks The issue of food contamination with mycotoxins is a serious problem worldwide. These substances are highly toxic to humans and chronic effects on the human organism in very low doses may cause long-term medical complications. In our work we focused on one of the most common dietary mycotoxins - patulin, which is found in apples and related products (especially apple juices). High performance liquid chromatography (HPLC) coupled with on- line solid phase extraction (SPE) using a column switching technique for sample treatment was developed for determination of mycotoxin patulin in apple drinks and juices. A volume of 250 µl of juice sample was injected directly into the on-line SPE-HPLC system. After injection of the sample the extraction of patulin from juice matrix was carried out on SPE precolumn. SPE precolumn 25 x 3 mm was filled with Supel MIP Patulin sorbent, which is a specific "molecularly imprinted polymer" (MIP) designated for the selective extraction of patulin from an apple matrix. As the...
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On-line SPE HPLC method optimization for determination of patulin mycotoxin in apple drinks
Holznerová, Anežka ; Šatínský, Dalibor (advisor) ; Solich, Petr (referee)
Charles University in Prague Faculty of Pharmacy in Hradec Králové Department of Analytical Chemistry Candidate: Bc. Anežka Holznerová Supervisor: Doc. RNDr. Dalibor Šatínský, Ph.D. Title of Diploma Thesis: On-line SPE HPLC method optimization for determination of patulin mycotoxin in apple drinks The issue of food contamination with mycotoxins is a serious problem worldwide. These substances are highly toxic to humans and chronic effects on the human organism in very low doses may cause long-term medical complications. In our work we focused on one of the most common dietary mycotoxins - patulin, which is found in apples and related products (especially apple juices). High performance liquid chromatography (HPLC) coupled with on- line solid phase extraction (SPE) using a column switching technique for sample treatment was developed for determination of mycotoxin patulin in apple drinks and juices. A volume of 250 µl of juice sample was injected directly into the on-line SPE-HPLC system. After injection of the sample the extraction of patulin from juice matrix was carried out on SPE precolumn. SPE precolumn 25 x 3 mm was filled with Supel MIP Patulin sorbent, which is a specific "molecularly imprinted polymer" (MIP) designated for the selective extraction of patulin from an apple matrix. As the...
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