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Determination of Lactulose by capillary zone electrophoresis
Holcová, Kristýna ; Doležal, Martin (advisor) ; Kučera, Radim (referee)
Determination of Lactulose by capillary zone electrophoresis Kristýna Holcová A lot of sugar analysis methods exist. Capillary Electophoresis (CE), High Performance Liquid Chromatography (HPLC), Gas Chromatogramy (GC), Thin-Layer Chromatography (TLC) etc. HPLC is the method which is most used, however other methods have also some advantage. One of the already described capillary electrophoresis methods is the carbohydrate separation in alkaline medium after chelatation with copper(II) ions with direct UV detection without need of previous derivatization. It has been tested for simple sugars, disaccharides, some amine-acids and inorganic cations. I applied capillary electrophoresis method mentioned above on questions of setting up osmotic laxancium lactulose. Lactulose is disaccharide from the chemistry point of view. It appears in molasses form in commercial medicaments. The method has been suited to the apparature used during the research and tested substance. It has been proved the specifity and accuracy of the method and it has been set up correlative factor (0,9994). Quality and quantity analysis has been applied on chosen samples.
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Determination of Lactulose by capillary zone electrophoresis
Holcová, Kristýna ; Doležal, Martin (advisor) ; Kučera, Radim (referee)
Determination of Lactulose by capillary zone electrophoresis Kristýna Holcová A lot of sugar analysis methods exist. Capillary Electophoresis (CE), High Performance Liquid Chromatography (HPLC), Gas Chromatogramy (GC), Thin-Layer Chromatography (TLC) etc. HPLC is the method which is most used, however other methods have also some advantage. One of the already described capillary electrophoresis methods is the carbohydrate separation in alkaline medium after chelatation with copper(II) ions with direct UV detection without need of previous derivatization. It has been tested for simple sugars, disaccharides, some amine-acids and inorganic cations. I applied capillary electrophoresis method mentioned above on questions of setting up osmotic laxancium lactulose. Lactulose is disaccharide from the chemistry point of view. It appears in molasses form in commercial medicaments. The method has been suited to the apparature used during the research and tested substance. It has been proved the specifity and accuracy of the method and it has been set up correlative factor (0,9994). Quality and quantity analysis has been applied on chosen samples.
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Vzájemné interakce mezi psy na procházkách v Přerově
Holcová, Kristýna
The thesis was focused on canine interactions in public spaces. Three hundred canine dyads was observed. The gender of the dog affected the initiation of sniffing. The gender of the dog and leading of dogs had an effect on the location of sniffing on the body of dogs. Number of sniffing places was affected by day time and leading of dogs. Playful interactions were affected by the place of observation and leading of dogs. The gender of the dog and it's owner had an impact on marking the territory. In conclusion, the results indicate that canine interactions are influenced by a number of factors.
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