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Signs of the temporolimbic dysfunction in gamblers
Hončová, Veronika ; Herink, Josef (advisor) ; Kovařík, Miroslav (referee)
Charles University of Prague Faculty of Pharmacy in Hradec Králové Department of Biological and Medicinal Sciences Student: Bc. Veronika Hončová Supervisor: Doc. MUDr. Josef Herink, DrSc. Consultant: MUDr. Jela Hrnčiarová Title: Signs of the temporolimbic dysfunction in gamblers Background: The aim of this thesis was to determine whether pathological gamblers exhibit symptoms related to epileptic spectrum disorder. Methods: To investigate, symptoms of the temporolimbic dysfunction were examined by means of the structured questionnaires CPSI (Complex Partial Symptoms Inventory) and LSCL-33 (Limbic System Checklist-33). Mann-Whitney U test and Spearman's correlation coefficient were used for statistical evaluation. Results: The mean score in the questionnaire CPSI of gamblers was 38,9 points, which is an abnormal score. With the questionnaire LSCL-33, the mean score of 21,5 points, corresponds to suspected findings. From the mean results it can be concluded that pathological gamblers may have a higher incidence of the symptoms of the temporolimbic dysfunction. When comparing the groups of gamblers with smokers, it was evaluated that in the questionnaire CPSI was a statistically significant difference between the appearances of symptoms of epileptic spectrum disorder, while it was not in the...
Cholinesterase activity following experimentally induced organophosphorus poisoning.
Svobodová, Irena ; Herink, Josef (advisor) ; Kuča, Kamil (referee)
The aim of present work was a comparison of effectiveness of oximes chosen in the experimental therapy of nerve agents intoxication (soman and russian VX). All experiments were performed in male Wistar rats. All oximes tested were administered intramuscularly either in a separate injection or in combination with atropine. The preparation of the brain parts for determination of acetylcholinesterase was performed in several time intervals after drugs tested administration. Antidotal potency of oxime HI-6 was superior in comparison with the rest of oximes tested.
Transport mechanisms of drugs trough the blood-brain barrier and their influencing by L-carnitine.
Havelek, Radim ; Herink, Josef (advisor) ; Kudláčková, Zděnka (referee)
Many neurodegenerative diseases, cancer and infections of the brain become more prevalent as population become older. That is one of the reasons, why blood-brain barier and it's transport mechanisms are intensively investigated. The blood-brain barrier is a unique membranous barrier that tightly segregates the brain from the circulating blood. The blood- brain barrier is formed by the connection of closely adjacent tight junctions between the capillary endothelial cells, which are not fenestrated and which display minimal pinocytosis. The capilary endothelial cells form a polarized barrier, which regulates transport of molecules across the blood-brain barrier. L-carnitine is compound necessary in the peripheral tissues for a transfer of fatty acids for their oxidation within the cell, it accumulates in the brain despite low β-oxidation in this organ. In order to enter the brain, L-carnitine has to cross the blood- brain barrier via specific carnitine transporters. The aim of this work was to describe recent information about blood-brain barrier, L-carnitine, transport mechanisms across blood-brain barrier and to find whether administration of L-carnitine can affect effect of 7- methoxytacrine. As a marker of the effect of drugs tested was chosen determination of activity of AChE by Ellman method in...
Testing antioxidant properties of selected acetylcholinesterase inhibitors
Kucková, Jana ; Vopršalová, Marie (advisor) ; Herink, Josef (referee)
Charles University Faculty of Pharmacy in Hradec Králové Department of Pharmacology & Toxicology Student: Jana Kucková Supervisor: PharmDr. Marie Vopršalová, CSc. Title of diploma thesis: Testing antioxidant properties of selected acetylcholinesterase inhibitors Very widespread Alzheimer's disease is typical by a complex pathology. Its causality is not clearly understood. There is increasing discussion due to the negative impact of free radicals on this disease formation. There are testing new substances that have also antioxidant properties, in addition to their classical effects. The aim of this diploma thesis is spectrophotometric testing of antioxidant properties of selected acetylcholinesterase inhibitors. It is based on DPPH test where the presence of antioxidants with antiradical activity causes the reduction of the color stable 2,2-diphenyl-1- picrylhydrazyl (DPPH) radical to a colorless molecule. The principle of the method is the measurement of decrease in absorbance (decolorization) of selected substances that are directly proportional to the antioxidant (antiradical) efficacy of the analyzed inhibitors. It focuses on twelve samples, Phenothiazine, which is the leader structure of new inhibitors synthesis, and two standards. Antioxidant activity is expressed as the effective...
The effect of acetylcholinesterase reactivators on urinary bladder contraction
Rambousková, Lucia ; Vopršalová, Marie (advisor) ; Herink, Josef (referee)
Candidate: Mgr. Lucia Rambousková Title of thesis: The effect of acetylcholinesterase reactivators on urinary bladder contraction Charles University in Prague, Faculty of Pharmacy in Hradec Králové Department of Pharmacology and Toxicology Supervisor: PharmDr. Marie Vopršálová, CSc. Consultant specialist: PharmDr. Vendula Šepsová, Ph.D Acetylcholinesterase (AChE) reactivators major mechanism is attributed to their ability to reactivate phosporylated enzyme, AChE. It has been thought that oxime might have additional direct pharmacological effect, other than AChE reactivation. The oximes may exert effects by interaction with cholinergic receptors. The most of them have antiniconitic or antimuscarinic properties, thus they protect against higher concentration of acetylcholine. The aim of this study was to evaluate the effect of reactivators K 203 and K 027. A contraction study on the rat urinary bladder has been chosen to investigate effect on muscarinic and purinergic receptors. Isolated strips of urinary bladder in the organ bath were used. There were three types of stimulation - electric stimulation (EFS), muscarinic receptor agonist methacholine (MCh) and purinergic receptor agonist adenosine triphosphate (ATP). In conclusion oxime K 027 has tendency to reduce the contraction (during EFS and MCh...
Study of antileptic drug dispensation in pharmacy with enlarged sphere of activity in years 2005 - 2006
Sedláček, Vladimír ; Vopršalová, Marie (advisor) ; Herink, Josef (referee)
This thesis aims to compare consumption of particular antiepileptics (N03A) in two following years 2005 and 2006 in pharmacy with extended sphere of action to confirm the presumption of continuing increase of new antiepileptics consumption. Using the pharmacy system Paenium the consumption of particular antiepileptics was recorded as a number of drugs actually demanded by patients based on medical prescription. ATC/DDD system valid in year 2006 has been used to express the consumption. Consumption of particular antiepileptics groups was expressed as a sum of expended DDD of all active substances from each particular group of antiepileptics in years 2005 and 2006. Percentage share of particular antiepileptics groups has been calculated from the total consumption in both years. Consumptions of particular antiepileptics groups in years 2005 and 2006 have been compared. Furthermore, consumption of particular active antiepileptic substances was calculated for the years 2005 and 2006 as a sum of DDD of all medicinal product packages with content of each individual active antiepileptic substance. Since a new anti-epileptic drug pregabalin entered the Czech market in 2005, significant increase of it's consumption in first months was assumed. It was interesting to observe the influence of the new marketed drug on...
JNK3 MAP-kinase localization in the brain of mice with Alzheimer's disease
Kohoutová, Lucie ; Rathouská, Jana (advisor) ; Herink, Josef (referee)
Charles University in Prague Faculty of Pharmacy in Hradec Králové Department of Biological and Medical Sciences Student: Lucie Kohoutová Supervisor: PharmDr. Jana Rathouská, Ph. D. Specialized supervisor: Dr. Maria Javier Ramirez Title of diploma thesis: JNK3 MAP-kinase localization in the brain of mice with Alzheimer's disease Background: The pathogenesis of Alzheimer's disease (AD) is incompletely understood. Discovery of c-Jun N-terminal kinases (JNKs), involved in regulation of gene expression, inflammation, cell proliferation and apoptosis, may contribute to better understanding of AD pathogenesis. JNK3 is a brain-specific JNK isoform involved in apoptosis of mammalian neurons. Precise localization of JNK3 in the brain still remains unclear. JNK3 silencing achieved by gene knockout had directly decreased beta amyloid (Aβ) levels in previous AD models in mice. We performed an immunohistological study to localize JNK3 gene in the mice brain in order to better focus future gene targeting therapy. Methods: A transgenic mice model of AD (Tg2576) has been used to study the expression of JNK3 by western blotting compared to the wild type. In the same brains localization of pJNK3 in astroglia (GFAP), microglia (Ox42), neurons (NeuN), senile plaques (Aβ) or pTau, using immunohistochemistry staining...
Pharmacotherapy of anxiety and sleep disorders
Böhmová, Kateřina ; Hrdina, Radomír (advisor) ; Herink, Josef (referee)
Charles University in Prague Faculty of Pharmacy in Hradec Králové Department of Pharmacology & Toxicology Student: Kateřina Böhmová Supervisor: Prof. MUDr. Hrdina Radomír, CSc. Title of diploma thesis: Pharmacotherapy of anxiety and sleep disorder Anxiety and sleep disorders belong to common medical conditions. Anxiety attacks are recurring unpleasant emotion states. Sleep disorders are characterized by insufficient or excessive amount of sleep or its poor quality, which persist for significant time period. These common disorders represent important medical and socioeconomic problem. Opinions about etiopathogenesis and classification are constantly evolving. Women and elders suffer from these disorders more often. Complex treatment includes mostly removing the cause, life style changes, psychotherapy, and pharmacotherapy of acute attacks relieving anxiety or sleep problem and prophylactic pharmacotherapy slowly lowering frequency, intensity and later on optimally total resolution of symptoms. Antidepressants from SSRI or SNRI group are considered to be the drugs of first choice in frequent anxiety and chronic sleep disorders. Before the full effect of these antidepressants, it is possible to use BZD for acute attacks treatment. For acute sleep disorders treatment, so called "Z" drugs could be...
A Potential Neuroprotective Action of Ghrelin on Dopaminergic Neurons Transplanted into the 6-hydroxydopamine Lesioned Rat
Zayaková, Kristýna ; Fikrová, Petra (advisor) ; Herink, Josef (referee)
Parkinson's disease is a neurodegenerative chronic disorder which is characterised by loss of dopamine in nigrostriatal pathway in the brain. One of the alternative therapies is transplantation of embryonic ventral mesencephalon tissue to the affected brain area but currently grafted cells do not survive the transplantation process very well. Ghrelin, which has neuroprotective properties could be a potential additional treatment after transplantation with the intention of increasing the number of live cells in the graft. The aim of this study was to elucidate a presence of ghrelin receptor in embryonic ventral mesencephalon used in transplantation and assess behavior of rats after transplantation. Immunocytochemistry was performed for ghrelin receptor, ghrelin-O-acetyl transferase, tyrosine hydroxylase and for neural and stem cell markers in embryonic ventral mesencephalon suspension. 50 Sprague-Dawley rats with 6-hydroxy- dopamine lesions were split into five groups; Group A was non-transplanted group, other groups underwent transplantation of embryonic ventral mesencephalon and received diverse treatments (ghrelin agonist, low doses of ghrelin, high doses of ghrelin or saline1 ). Behavior of all animals was assessed by motor tests pre- transplantation and 4 weeks, 6 week and 8 weeks after...

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See also: similar author names
1 HERINK, Jakub
1 Herink, Jaroslav
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