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The methodology of integrated Colorado potato beetle management in potatoes under new agro-environmental conditions
Doležal, P. ; Hausvater, E. ; Baštová, P. ; Kocourek, F. ; Stará, J. ; Sedlák, P. ; Sedláková, V. ; Skoková Habuštová, Oxana ; Nermuť, Jiří
The aim of the certified methodology is to provide updated principles of effective integrated Colorado potato beetle management for the agricultural practice resulting from new findings on biology and population genetics in the situation, when the range of effective insecticides has been reduced, pest resistance to insecticides has been increased and agro ecological conditions have been changed. Colorado potato beetle is a very serious insect pest of potatoes, highly mobile and able to quickly develop insecticide resistance. The methodology was drawn up based on the newest findings on the efficacy of existing insecticides registered against Colorado potato beetle, taking into account newly acquired knowledge in the field of pest resistance to active ingredients and a necessity of ever increasing multiple applications.
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Optimalization of the ELISA method for determination the content of Cry 3Bb1 toxin in the different parts of maize plant by commercial kit
Habuštová, Oxana ; Sehnal, František ; Svobodová, Zdeňka ; Hussein, Hany
This publication provides information on Enzyme Linked Immunosorbent Assay (ELISA) that is widely used in biological research, medicine, and industry for quantification of diverse antigene compounds. The major purpose of this book guide is a detail description of the ELISA usage for the monitoring of Cry toxins in the plant tissues of genetically modified crops. The Cry toxins are products of Bacillus thuringiensis genes used in plant transformations to make them resistant against specific insect pests. In our work we use ELISA to compare Cry content in different parts of the genetically modified maize during the growing season. We hope that this guide will simplify usage of ELISA not only for the measurements of Cry toxins but also for the analysis of other compounds present in plants naturally and as a result of transgenosis.
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