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Enzymatic system of anaerobic rumen fungi
Novotná, Zuzana ; Fliegerová, Kateřina (advisor) ; Homolka, Petr (referee) ; Javorský, Peter (referee)
Anaerobic fungi inhabiting the digestive tract of large herbivores secrete a variety of hydrolytic enzymes, including cellulases, xylanases, mannases, esterases, glucosidases, and glucanases, which effectively hydrolyze plant biomass consisting mainly of cellulose and hemicellulose, the most abundant polysaccharides in the biosphere. The immense progress has been achieved in investigation of Neocallimastix, Piromyces, however other genera are less studied. This work therefore concentrated the effort to analyze the hydrolytic system of genera Anaeromyces, Orpinomyces, Caecomyces and fungus KF9 described as new type of fungus belonging to the genus Piromyces. The studied anaerobic fungi grown in batch culture on M10 medium with rumen fluid and microcrystalline cellulose as carbon source produced a broad range of enzymes requisite for degradation of plant structural and storage carbohydrates including cellulase, endoglucanase, xylanase, α-xylosidase, β-xylosidase, α-glucosidase, β-glucosidase, β-galactosidase, mannosidase, cellobiohydrolase, amylase, laminarinase, pectinase and pectate lyase. These enzymes were detected in both the intra- and extra-cellular fractions, but production into the medium was prevalent with exception for intracellular β-xylosidase, chitinases, N-acetylglucosaminidase, and...
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Bacteriophage 936 by qPCR quantification in dairy products.
Olša Fliegerová, Kateřina ; Mrázek, Jakub ; Štrosová, Lenka ; Němečková, I. ; Kopečný, Jan
Virulent bacteriophages are still representing significant threat in dairy production even with frequent disinfection and sterilization all the equipment in dairy plants. Therefore a reliable \nmethod of phage monitoring and quantification necessary part of phage prevention. Method is based on qPCR amplification and fluorescent detection of copies number of the phage main\nstructural protein and therefore is independent on the phage cultivation. It is quick, reliable a sensitive enough to estimate phage copy numbers in dairy starters and whey in any phase of dairy production. \n
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Enzymatic system of anaerobic rumen fungi
Novotná, Zuzana ; Fliegerová, Kateřina (advisor) ; Homolka, Petr (referee) ; Javorský, Peter (referee)
Anaerobic fungi inhabiting the digestive tract of large herbivores secrete a variety of hydrolytic enzymes, including cellulases, xylanases, mannases, esterases, glucosidases, and glucanases, which effectively hydrolyze plant biomass consisting mainly of cellulose and hemicellulose, the most abundant polysaccharides in the biosphere. The immense progress has been achieved in investigation of Neocallimastix, Piromyces, however other genera are less studied. This work therefore concentrated the effort to analyze the hydrolytic system of genera Anaeromyces, Orpinomyces, Caecomyces and fungus KF9 described as new type of fungus belonging to the genus Piromyces. The studied anaerobic fungi grown in batch culture on M10 medium with rumen fluid and microcrystalline cellulose as carbon source produced a broad range of enzymes requisite for degradation of plant structural and storage carbohydrates including cellulase, endoglucanase, xylanase, α-xylosidase, β-xylosidase, α-glucosidase, β-glucosidase, β-galactosidase, mannosidase, cellobiohydrolase, amylase, laminarinase, pectinase and pectate lyase. These enzymes were detected in both the intra- and extra-cellular fractions, but production into the medium was prevalent with exception for intracellular β-xylosidase, chitinases, N-acetylglucosaminidase, and...
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