National Repository of Grey Literature 12 records found  1 - 10next  jump to record: Search took 0.00 seconds. 
Potential of mesenchymal stem cells and nanoparticles in the treatment of retinal disorders
Kettner, Ondřej ; Heřmánková, Barbora (advisor) ; Filová, Eva (referee)
Retinal degenerative diseases are recognized worldwide as one of the predominant causes underlying irreversible visual impairment and even blindness. The current standard treatment has helped in both the prevention and supportive treatment of several pathologies, especially if they relate to the retina. However, many of these methods are rather invasive in terms of procedure with the subsequent possibility of infection, inflammation or even retinal detachment with the potential risk of vision loss. These restrictions represent a significant cost to patients' quality of life and also have an economic impact on the healthcare system, so new treatment options are being sought. Mesenchymal stem cells (MSCs) are a promising cell therapy tool for the treatment of many previously untreatable defects and diseases. In addition to MSCs, the use of nanoparticles and nanofibrous carriers is currently being studied in the diagnosis and therapy of retinal diseases. Combining the immunomodulatory and regenerative properties of MSCs and nanotechnologies offers a promising therapeutic strategy for a number of diseases. This thesis is therefore devoted to the current state of the use of nanomaterials, MSCs and their combination that may provide new opportunities in the treatment of retinal degenerative diseases.
Smart biomaterials for connective tissues regeneration
Hefka Blahnová, Veronika ; Filová, Eva (advisor) ; Maxová, Hana (referee) ; Motlík, Jan (referee)
Connective tissues are characterized by significant volume of extracellular matrix. Their main role is to provide a mechanical support and protection to other body organs. This thesis is focused on regeneration of bone, cartilage and osteochondral defect. In the experimental part we observed viability and differentiation of human mesenchymal stem cells. In vitro we evaluated the potential of PCL scaffold with addition of growth factors, bone xenograft with biomimetic peptides, collagen I composite with bioceramics and a titanium alloy with nanostructured surface. During following in vivo study, we implanted a cell-free scaffold made of PCL, calcium phosphate and IGF-1, bFGF, TGFβ1 and BMP-2 to osteochondral defect. Unfortunately, addition of growth factors resulted in pathological inflammatory process despite clear beneficial effect in vitro. Likewise, the biomimetic peptide sequences promoted osteogenic differentiation of human mesenchymal stem cells. Addition of certain bioceramics influenced the scaffold morphology in the manner of pore size. However, we did not observe any effect of the surface characteristics on cell behavior. The cells were influenced rather by certain material. On the other hand, surface modification of titanium scaffold by anodic oxidation revealed that the most suitable...
Coculture of cells in vitro for bone regeneration
Sloupová, Lenka ; Filová, Eva (advisor) ; Tlapáková, Tereza (referee)
Cocultivation of two (or more) cell types in vitro leading to the formation of functioning bone tissue, later inserted into the damaged area, could be a solution for patients for whom the current methods (e.g. use of human bone grafts) are insufficient. In coculture, one cell type is used to accomplish osteogenesis, while the other is used for angiogenesis, because the limit of diffusion of O2 and essential nutrients is only 200 μm, which means that establishing a vascular network in vitro should prevent the new bone tissue from dying after implantation. Creation and understanding of a functioning coculture in vitro are crucial for developing a coculture successful in vivo. This work summarises and compares information about the influence of in vitro cocultivation on proliferation, osteogenesis and angiogenesis in coculture which uses osteoblasts (or osteoprogenitors), bone marrow mesenchymal stem cells (BMSC) or adipose derived mesenchymal stem cells (ADSC) as it's osteogenic cell type combined with various endothelial cell types. In order to understand the impact of cocultivation on these processes, one chapter deals with interactions between cocultured cell types. Keywords coculture, osteogenesis, angiogenesis, in vitro, osteoblasts, BMSC, ADSC
Mesenchymal stromal cells and biological scaffolds for neural tissue regeneration
Kočí, Zuzana ; Kubinová, Šárka (advisor) ; Filová, Eva (referee) ; Zach, Petr (referee)
Despite tremendous progress in medicine, injuries of the adult central neural system remain without satisfactory solution. Regenerative medicine employs tissue engineering, cellular therapies, medical devices, gene therapy, or growth factors with the aim to bridge the lesion, re-establish lost connections and enhance endogenous repair in order to restore neural function. The aim of my thesis was to evaluate therapeutic potential of two approaches, transplantation of human mesenchymal stromal cells (hMSCs) and biological scaffolds derived from extracellular matrix (ECM) for neural regeneration, particularly in models of spinal cord injury (SCI). First, hMSCs from various sources - bone marrow (BM), adipose tissue (AT) and Wharton's jelly (WJ) - were isolated and characterized in vitro. All cell types met the minimal criteria for MSC phenotype and displayed similar properties in terms of their surface marker expression, differentiation potential, migratory capacity, and secretion of cytokines and growth factors. On the other hand, the cell yield from WJ and AT was significantly higher, and MSCs isolated from these tissues proliferated better than from BM. Therapeutic effect of intrathecal application of hWJ-MSCs was then evaluated in SCI compression model in rats. The effect of low (0.5 million) and...
Regulation of bone formation using osteogenic supplements in an osteoporotic model
Krčmářová, Eliška ; Filová, Eva (advisor) ; Zadražil, Zdeněk (referee)
Osteoporosis is a disease of the bone metabolism which is characterised with a decrease of bone substance. The cause of this disease is the imbalance between the creation of a new bone substance by osteoblasts and the resorption of a bone tissue by osteoclasts, in favour of the bone resorption. The risk group of the development of this disease are women after menopause, who naturally register a decline of the estrogen hormone. Estrogen operates as an inhibitor of proosteoclastic factors such as receptor activator of NFκB ligand (RANKL), interleukin (IL)-1, IL-6 or TNF-α. The imbalance of the bone metabolism can also be caused by a disbalance in the production of Prostaglandin E2 (PGE2) and 1α,25-dihydroxyvitamin D3. They are strong mediators which can both stimulate and inhibit an osteoclastogenesis in vitro in concordance with the conditions of the culture/co-culture. This thesis focuses on the examination of an influence of those mediators (PGE2 in the concentration of 10-6 M and 10-8 M; 1α,25-dihydroxyvitamin D3 in the concentration of 10-8 M and 10-9 M) on the osteoclastogenesis from the rat PBMC at the presence of osteoblasts, with or without the combination of proosteoclastic factors macrophage colony-stimulating factor (M-CSF) and RANKL. Osteoclastogenesis was stimulated if PGE2 and...
Functionalized chitosan-based nanofibres for accelerated regeneration of soft tissues
Bazikov, Philipp ; Filová, Eva (advisor) ; Kössl, Jan (referee)
To improve the regeneration of soft tissues, wound dressings were developed, containing nanofibers, different in composition and functional features. The work concentrates on chitosan and composite scaffolds containing chitosan. Porous nanofibre materials based on chitosan are very promising for the regeneration of soft tissues. Chitosan exhibits pH-sensitive behavior due to the large number of amino groups on its chains, which makes chitosan nanofibres promising carriers for the delivery of drugs. In this work, the examples of different modifications of nanofibres are shown. To increase the efficiency and accelerate the regeneration of soft tissues, nanofibres are functionalized with bioactive substances of various types: antimicrobial, analgesic, growth factors, etc. To stabilize nanofibres and to improve the physical characteristics, treatments using glutaraldehyde, glyoxal, genipin or heat treatment were used. Chitosan is used to inhibit fibroplasia during wound healing and to promote cell growth and differentiation. The efficiency of antibacterial activity of chitin-glucan complex with nanofibres for wound healing was shown. Reconstruction of deeper wounds, in which skin and soft tissues are damaged, requires measures for spatial reconstruction and stimulation of regeneration processes in the...
Nanodiamonds as an innovative system for intracellular delivery of mirna-34a inprostatic cancer therapy
Bitti, G. ; Abate, M. ; Neuhoferová, Eva ; Kindermann, Marek ; Petráková, V. ; Boccellino, M. ; Quagliuolo, L. ; Filová, Eva ; Benson, Veronika ; Caraglia, M. ; Amler, Evžen
The microRNA(miRNA)-34a is an important regulator of tumor suppression. It controls the expression of several target proteins involved in cell cycle, differentiation and apoptosis, and antagonizes processes that are necessary for basic cancer cell viability as well as cancer stemness, metastasis, and chemoresistance. It is downregulated in numerous cancer types, including prostatic cancer, and inhibits malignant growth by repressing genes involved in various oncogenic signaling pathways. Given the anti-oncogenic activity of miR-34a, here we proved the substantial benefits of a new therapeutic concept based on nanotechnology delivery of miRNA mimics. In order to monitor the miRNA-34a replacement, we used a fluorescent nanodiamond particles (FND) system with linked miRNA-34a mimic, which was delivered to PC3 and DU145 prostatic cancer cell lines. We used functionalized nanodiamonds coated with polyethylenimine to transfer miRNA-34a into PC3 and DU145 prostatic cancer cell lines and we measured the zeta-potential of these complexes before using them for in vitro experiments. A replacement of miRNA-34 was observed by monitoring levels of miRNA-34 via real-time PCR. Moreover, our in vitro experiments demonstrated that miRNA-34a replacement, using this FND delivery system, decreased viability and induced apoptosis in prostatic cancer cell lines. Our findings suggest the replacement of oncosuppressor miRNA-34a provides an effective strategy for cancer therapy and the FND-based delivery systems seems to be an excellent strategy for a safe and effective targeting of the tumor.
Mesenchymal stromal cells and biological scaffolds for neural tissue regeneration
Kočí, Zuzana ; Kubinová, Šárka (advisor) ; Filová, Eva (referee) ; Zach, Petr (referee)
Despite tremendous progress in medicine, injuries of the adult central neural system remain without satisfactory solution. Regenerative medicine employs tissue engineering, cellular therapies, medical devices, gene therapy, or growth factors with the aim to bridge the lesion, re-establish lost connections and enhance endogenous repair in order to restore neural function. The aim of my thesis was to evaluate therapeutic potential of two approaches, transplantation of human mesenchymal stromal cells (hMSCs) and biological scaffolds derived from extracellular matrix (ECM) for neural regeneration, particularly in models of spinal cord injury (SCI). First, hMSCs from various sources - bone marrow (BM), adipose tissue (AT) and Wharton's jelly (WJ) - were isolated and characterized in vitro. All cell types met the minimal criteria for MSC phenotype and displayed similar properties in terms of their surface marker expression, differentiation potential, migratory capacity, and secretion of cytokines and growth factors. On the other hand, the cell yield from WJ and AT was significantly higher, and MSCs isolated from these tissues proliferated better than from BM. Therapeutic effect of intrathecal application of hWJ-MSCs was then evaluated in SCI compression model in rats. The effect of low (0.5 million) and...
Learning styles of students of English in Higher Education
FILOVÁ, Eva
This thesis is concerned with learning styles of students of English at Faculty of Education of the University of South Bohemia in České Budějovice. Its aim is to find out whether the students are aware of their perceptual learning style (auditory, visual, kinesthetic), which activities they use to encourage English language development (watching films or reading books in English, listening music, practising language on the Internet,), which type of environment they prefer and whether they are satisfied with teaching styles of their professors. A specifically made non-standardized questionnaire is used as a method of research. The thesis is traditionally divided into theoretical and practical parts. The theoretical part deals with a profile of basic terms related to the topic, classification of learning styles and possible ways of their diagnostics. The practical part is focused on the analysis of questionnaire research, whose findings are graphically demonstrated in diagrams with comments.
Functionalized nanofibers for accelerated regeneration of soft tissues
Bazikov, Philipp ; Filová, Eva (advisor) ; Krulová, Magdaléna (referee)
To improve the regeneration of soft tissues, wound coatings were developed, containing nanofibres, different in composition and functional features. The work concentrates on chitosan and composite scaffolds containing chitosan. Porous nanofiber materials based on chitosan are the most promising nanofibers for the regeneration of soft tissues. Chitosan exhibits pH-sensitive behavior due to the large number of amino groups on its chains, which makes chitosan nanofibers promising carriers for the delivery of macromolecules. To increase the efficiency and accelerate the regeneration of soft tissues, nanofibers are functionalized with bioactive substances of various types: antimicrobial, analgesic, growth factors, etc., as well as functional polymers. To stabilize nanofibers and to improve the physical characteristics, treatment using glutaraldehyde, glyoxal, genipin or heat treatment are used. Chitosan is used to inhibit fibroplasia during wound healing and to promote tissue growth and differentiation in tissue culture. The efficiency of antibacterial activity of chitin-glucan complex with nanofibers for wound healing was shown. Reconstruction of deeper wounds, in which skin and soft tissues are damaged, requires measures for spatial reconstruction and stimulation of regeneration processes in the...

National Repository of Grey Literature : 12 records found   1 - 10next  jump to record:
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2 FILOVÁ, Eva
3 Filová, Elena
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