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Analysis of streptomycete isolates carrying a cyclizing aminolevulinate synthase gene
Rašmanová, Petra ; Petříčková, Kateřina (advisor) ; Felsberg, Jurgen (referee)
Streptomyces bacteria are well-known producents of many bioactive metabolites. Its secondary metabolism is a source of many important groups of active compounds that are recently investigated by means of many new methods based on bioinformatic analyses of genome data, modern LC-MS techniques, and metabolic modeling methods. This thesis originates from the genetic screening for a specific gene (als) for cyclizing 5-aminolevulinate synthase. Based on earlier studies, we consider this gene as a genetic tag of the producers of secondary metabolites containing the C5N unit (2-amino-3-hydroxycyklopent- 2-enon). Such metabolites include several groups with variable structures and biological activities, which include manumycins as well. Manumycins are small polyketides with a weak antibiotic aktivity, especially against gram-positive bacteria. However, its cancerostatic and anti-inflammatory effects are of greater importance. Streptomyces monomycini BCCO10 1552 and Streptomyces capoamus BCCO10 1636 strains were found positive for the presence of the als gene in the targeted genetic screening. By the als phylogeny, they cluster near the producents of manumycin compounds. This thesis aimed to determine whether these new natural isolates produce any compounds containing C5N unit, and to characterize them in...
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Characterization of Corynebacterium glutamicum promoters responding to increase of temperature
Zemanová, Martina ; Nešvera, Jan (advisor) ; Lichá, Irena (referee) ; Felsberg, Jurgen (referee)
My thesis is focused on characterizationof Corynebacteriumglutamicum promoters responding to increase of temperature.C. glutamicum is the most importantrepresentativeof a gram-positive,non-pathogenic,amino-acidproducing bacteria.Its strainsare industrialproducersof glutamic acid, lysine, threonine, tryptophaneand phenylalanine(Liebl et al., l99l). Amino acids producedby microbial fermentationare used in medicine(as infusionliquids or therapeutics), chemical industry (as detergentsand cosmeticscompounds),food industry (as additives), agriculture and stockbreeding (as growth-regulatoradditives to the animalsfeed)(Hermann,2003). Althoughknowledgeof organizationsof its genesinvolvedin biosynthesis of variousaminoacidsincreasedconsiderablyduringlastyears,still littleis known about molecularsignals controllinggene expression. Since regulationof gene expressionon the level of transcriptionplays crucial role, understandingthe structureand functionof transcriptioninitiationsignals(promoters)is of primary importance.Promoters, which can be easily switahed on and off during fermentationsby externalfactorsmay ensurethe controlledoverexpressionofthe chosengenes.Therefore,the C. glutamicumpromotersrespondingto increaseof temperaturehavebeenisolated. Two differentapproachesfor isolationof the promoterswere employed. First...
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DNA sequencing
Felsberg, Jürgen
Performance of scientific and technical work largely consisting of DNA sequencing according to client specification. In adition all DNA analysis necessery for DNA sequencing are carried out.
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DNA sequencing
Felsberg, Jürgen
Performance of scientific and technical work largely consisting of DNA sequencing according to client specification. In adition all DNA analysis necessery for DNA sequencing are carried out.
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DNA sequencing
Felsberg, Jürgen
Performance of scientific and technical work largely consisting of DNA sequencing according to client specification. In adition all DNA analysis necessery for DNA sequencing are carried out.
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2 DNA sequencing
Felsberg, Jürgen
Performance of scientific and technical work largely consisting of DNA sequencing according to client specification. In adition all DNA analysis necessery for DNA sequencing are carried out.
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DNA sequencing
Felsberg, Jürgen
Performance of scientific and technical work largely consisting of DNA sequencing according to client specification. In adition all DNA analysis necessery for DNA sequencing are carried out.
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DNA sequencing
Felsberg, Jürgen
Performance of scientific and technical work largely consisting of DNA sequencing according to client specification. In adition all DNA analysis necessery for DNA sequencing are carried out.
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DNA sequencing
Felsberg, Jürgen
Performance of scientific and technical work largely consisting of DNA sequencing according to client specification. In adition all DNA analysis necessery for DNA sequencing are carried out.
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DNA sequencing
Felsberg, Jürgen
Performance of scientific and technical work largely consisting of DNA sequencing according to client specification. In adition all DNA analysis necessery for DNA sequencing are carried out.
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