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Influence of alternative teaching styles on social climate in the classroom
Dvořáková, Eva ; Hadj - Moussová, Zuzana (advisor) ; Duplinský, Josef (referee)
In the theoretical part of my graduation theses I occupy myself with the questions of the social climate of the class and with the alternative education, its history, function and its connection with the quality of social climate of classes, which are directed by the given style of education. The research part results from the investigation done at four types of school, which are working by different School education programs, finding out the social climate of the classes at this schools and searching connections between the climate of the classes and the pedagogical ideas, from which the school education program results.
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Use of plasma jet for wound healing
Dvořáková, Eva ; Márová, Ivana (referee) ; Skoumalová, Petra (advisor)
This diploma thesis was focused on the possibility of using a plasma nozzle to accelerate the wound healing process. The benefits of using low-temperature plasma in medicine or biomedical applications are known from many studies, and low-temperature plasma is already used to sterilize medical devices, materials or surgical instruments. Some studies also report a high potential of usinh plasma nozzle in the treatment of skin wounds. In the experimental part of this work, an in vitro wound healing test was performed using two different low-temperature plasma sources. Source No. 1 was a surface wave microwave discharge and source No. 2 was a torch microwave discharge. An in vitro scratch healing test was performed on a monolayer of HaCaT keratinocytes and testing was performed using various parameters. The influence of the plasma treatment time was monitored, as well as the influence of the plasma discharge power and also the influence of the argon working gas flow. Especially when using a torch microwave discharge, faster wound healing was recorded at most of the parameters used compared to the control. Thus, it can be said that this source appears to be potentially suitable for faster wound healing. Furthermore, in the work using the MTT cytotoxicity test, the viability of skin cells after their plasmination was also monitored using the same conditions as in the in vitro wound healing test. When performed in the standard MTT assay, none of the settings or sources used showed any cytotoxic effects on keratinocytes. LDH cytotoxicity tests were also performed concurrently to verify the accuracy of the MTT assays. The results of both tests agreed and the use of low-temperature plasma in skin treatment can be considered as safe. Overall, the results show that the plasma nozzle can find use in medicine in the healing of skin wounds and chronic defects as a potentially fast, inexpensive and effective method.
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Isolation and characterization of natural antimycotics and their potential applications
Dvořáková, Eva ; Hoová, Julie (referee) ; Skoumalová, Petra (advisor)
This bachelor thesis was focused on preparation and characterization of products containing natural antimycotic ingredients. The theoretical part summarizes the current issue of increased presence of mycoses and yeast diseases in connection with the extension of the problem of reduced immunity. Furthermore, the problem of synthetic drugs and antimycotics compared to alternatives of natural origin was described. In the experimental part, selected extracts (aqueous, ethanolic and lipidic) were prepared, characterized and subsequently encapsulated into liposomes. The content of polyphenols, flavonoids and the total antioxidant activity of extracts was measured. In liposomes, the encapsulation efficiency, size, stability and long-term stability were measured. In addition, the antimycotic activity of individual extracts and liposomes against Candida glabrata was studied. For comparison, the antifungal activity of the commercial drug clotrimazole was also tested. Lipidic extracts showed highest antimycotic activity from all of the prepared extracts, which is why these extracts were used to prepare antifungal gels and suppositories. More specifically, lipidic extracts of nasturcium, cinnamon, ginger and liposomes containing them were used. Their antimycotic activity was also tested and confirmed for these preparations. Finally, the cytotoxicity test on the HaCaT human cell line showed that used extracts and liposome particles are safe and that the prepared products could thus serve as an alternative to synthetically prepared antimycotics in the future.
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Molekulářně biologické změny u karcinomu endometria
Dvořáková, Eva ; Špaček, Jiří (advisor) ; Odrážka, Karel (referee) ; Sláma, Jiří (referee)
Molecular biological changes in endometrial carcinoma Endometrial cancer is the most common cancer of the female reproductive tract. The incidence has increased with lifestyle and environmental changes. Similar to other cancers, endometrial cancer has been shown to be a complex disease driven by different factors, including genetic and epigenetic alterations. Understanding these changes underlaying cancer development or progression is important for finding of new standards for both diagnosis and therapy of individual patients. The aim of the study was to evaluate selected molecular biological changes in endometrial carcinoma comparing to non-neoplastic endometrium. The first specific aim was to compare presence of K- ras mutation in early stages of endometrioid type of endometrial carcinoma with normal endometrium, and to evaluate association to clinical-pathological characteristics (tumor stage and grade). We analyzed 79 samples of endometrium (59 samples of endometrioid endometrial carcinoma stage I, and 20 samples of normal, non-neoplastic endometrium). Detection of K-ras mutation was made by using of K-ras StripAssay™ (ViennaLab Diagnostics GmbH). The frequency of K-ras mutation in the carcinoma group did not differ from the group of control samples (24% vs. 15%). No association between K-ras...
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Effect of zearalenone on reproductive parameters and the expression of selected genes in mice.
Dvořáková, Eva ; Pěknicová, Jana (advisor) ; Nedvídek, Josef (referee)
A number of chemicals may have a negative impact on the environment and wildlife. Endocrine disruptors (EDs), which can mimic estrogen, interfere with natural hormones in organism and can have a negative effect on the reproductive system. Such substances include zearalenone (ZEA) - mycotoxin, produced by the fungi Fusarium. Despite the non-steriodal structure of its molecule, ZEA and its derivates possess potent estrogenic activity. The influence of ZEA on reproductive parameters and changes in expression of selected genes were tested in the outbred line of mice. This study showed significant effects of ZEA on number of reproductive performances. This effect was observed at lower examined dose, to which are humans normally exposed, and at higher doses, both showed changes in the tested parameters.
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