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Změny střevního mikrobiomu u pacietů s idiotypickými střevními záněty léčenými pomocí anti-TNF-α
Damašková, Dagmar ; Mrázek, Jakub (advisor) ; Drbal, Karel (referee)
English Abstract Crohn's disease together with ulcerative colitis, is a type of inflammatory bowel disease (IBD) with increasing incidence and prevalence in developed countries. IBD is an immunologically mediated multifactorial disease and it's mechanism of action is still unknown. Current well- established treatment targets the inflammation with corticosteroids and immunosuppressive drugs. Apart from the intestinal inflammation, which is the primary target of the treatment, patients are characteristically afflicted with intestinal dysbiosis. Therefore, possible interventions might be an adjuvant or biological therapy. Adjuvant therapy directly aims the microbiota with probiotics, whereas the target of biological therapy is TNF-α, a pro- inflammatory cytokine excessively secreted by macrophages. The aim of this thesis is to evaluate intestinal microbiota composition changes in IBD patients with regard to courses of adjuvant and biological therapy. Bacterial diversity was analyzed using three different DNA extraction techniques. Rapid beat beating + column (RBB+C) was chosen for analyzing patient samples, as it showed the highest DNA yield and the highest DNA purity. Primarily the bacterial diversity was analyzed using degradation gradient gel electrophoresis (DGGE) with subsequent sequencing of bands of...
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Modelling assessment of scenarios of urban development to air quality and thermal comfort at street level
Resler, Jaroslav ; Krč, Pavel ; Belda, Michal ; Juruš, Pavel ; Benešová, N. ; Vlček, O. ; Damašková, D. ; Eben, Kryštof ; Derbek, P.
A new model for simulations of urban environment USM was developed and integrated into LES model PALM inside the project UrbanAdapt. The model allows simulations of air flow, radiation, air temperature and temperature of materials, air pollution and indices MRT and PET in fine resolution. The surrounding of a crossroad of the streets Komunardu and Delnicka in Praha-Holesovice was chosen as a pilot area in collaboration with IPR Prague. Studied scenarios included changes of housing development as well as assessment of different types of plant canopy and sensitivity studies of the colour of walls, roofs, streets and pavements.
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Development and validation of the new model of thermal conditions of urban environment in fine resolution
Resler, Jaroslav ; Krč, Pavel ; Belda, Michal ; Juruš, Pavel ; Benešová, N. ; Lopata, J. ; Vlček, O. ; Damašková, D. ; Eben, Kryštof ; Derbek, P. ; Maronga, P. ; Kanani-Sühring, F.
The assessment of different scenarios of the city development to air quality and thermal comfort in the areas of street canyons was our main goal inside the project UrbanAdapt. It follows the need for a model which allows to simulate air flows in fine resolution of the order of meter and realistically predict turbulence in the complex terrain of streets and buildings. The LES models comply with such requirements but the review showed that there was no free available LES model which could model the energy exchange in urban environment, i.e. the interaction of energy and air flows including effects of vegetation and different properties of urban surfaces and materials. Thus we decided to extend the existing LES model PALM by a new module USM (Urban Surface Model) which describes the most important energy exchanges in the urban environment. The validation of the model was done against observations obtained by IR camera in the course of heat wave episode in July 2015.
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Změny střevního mikrobiomu u pacietů s idiotypickými střevními záněty léčenými pomocí anti-TNF-α
Damašková, Dagmar ; Mrázek, Jakub (advisor) ; Drbal, Karel (referee)
English Abstract Crohn's disease together with ulcerative colitis, is a type of inflammatory bowel disease (IBD) with increasing incidence and prevalence in developed countries. IBD is an immunologically mediated multifactorial disease and it's mechanism of action is still unknown. Current well- established treatment targets the inflammation with corticosteroids and immunosuppressive drugs. Apart from the intestinal inflammation, which is the primary target of the treatment, patients are characteristically afflicted with intestinal dysbiosis. Therefore, possible interventions might be an adjuvant or biological therapy. Adjuvant therapy directly aims the microbiota with probiotics, whereas the target of biological therapy is TNF-α, a pro- inflammatory cytokine excessively secreted by macrophages. The aim of this thesis is to evaluate intestinal microbiota composition changes in IBD patients with regard to courses of adjuvant and biological therapy. Bacterial diversity was analyzed using three different DNA extraction techniques. Rapid beat beating + column (RBB+C) was chosen for analyzing patient samples, as it showed the highest DNA yield and the highest DNA purity. Primarily the bacterial diversity was analyzed using degradation gradient gel electrophoresis (DGGE) with subsequent sequencing of bands of...
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