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Stoichiometry of transport mediated by bacterial membrane transport protein MntH of E. coli
Doležalová, Iva ; Chaloupka, Roman (advisor) ; Marešová, Lydie (referee)
The Nramp family (Natural Resistance-Associated Macrophage Protein) includes secondary active transport proteins transporting divalent metal ions together with protons into a cell. Divalent metal ions play a major role in the cell metabolism and participate in many intracellular processes. We have studied bacterial Nramp ortholog - MntH (Proton-dependent Manganese Transporter), its transport stoichiometry and other functionally important characteristics. We have found that the external pH affected the transport stoichiometry. The functional differences between the wild type and the protein with mutation N401G appears only in lower external pH. Moreover, uncoupled proton flux mediated by MntH under specific experimental conditions is inhibited by calcium, whereas coupled proton flux increases after the addition of calcium ions. This can be connected to the fact that calcium is also transported by MntH. Surprisingly, the transport stoichiometry of neither the wild type nor the protein with mutation N401G is affected by the addition of calcium or the change of the external medium.
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Realization of moving aktivity in Kindergarten
DOLEŽALOVÁ, Iva
The dissertation deals with the importance and realization of exercise of nursery-age children in the current way of life, especially in the contemporary nursery school. In the theoretical part there is information about exercise in general, its meaning for the physical and psychical fitness, a brief characteristic of the organs of motion, nervous control of movement, and a brief development of motor control. Then there is description of physical abilities and skills and a characteristic of physical activities in relation with the contemporary nursery-age education. The last part deals with the pulse rate of nursery-age children and their reaction to exercise. The practical part deals with the measurement of the pulse rate of nursery-age children and their reaction to specific forms of the organization of physical activities. In the conclusion there are the results of the research and a recommendation of suitable ways of the realization of physical activities in the nursery school.
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