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Library for Operations over Finite Automata
Bartůněk, Petr ; Puš, Viktor (referee) ; Kaštil, Jan (advisor)
This work deals with two basic operations over finite automata. Determination of nondeterministic finite automata and minimization of deterministic finite automata. For these two operations I proposed sequential algorithms that are parallelizable. I deal mainly with finding the speedup of SSE instructions, or use the OpenMP library. The trend today is mainly in increasing the number of processors, so I propose parallel algorithms for multiple processors. When searching for the optimal solution, I will be to examine other ways to achieve speedup, for example efficient saving of the data structures in memory.
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Modular System of Casino
Bartůněk, Petr ; Rozman, Jaroslav (referee) ; Grulich, Lukáš (advisor)
This thesis is pointed to a modular development of hazard game system. There is one main module in this system, it services gamers database and user accounts. Every game in this system is module pair; first it is game server, it realizes the game, and second client application, it has user interface for this game. Every network communications are realized using my own multi platform network library TCP_Network, and using network protocol for every server.
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Danio rerio as a model of serious human diseases
Hason, Martina ; Bartůněk, Petr (advisor) ; Živný, Jan (referee) ; Divoký, Vladimír (referee)
(ENGLISH) Over the last five decades, zebrafish (Danio rerio) has become a useful vertebrate model organism for the field of developmental biology and disease control. Using zebrafish in xenotransplantation studies is becoming more popular and progressed towards drug screening of anti-cancer drugs. Zebrafish are particularly suitable for high-throughput pre-clinical drug screening, due to the small size of embryos and the striking evolutionary conservation of cancer- related pathways between human and zebrafish. The fast, large-scale evaluation of the cancer- drug response in vivo could facilitate progress in personalized cancer therapy. Nevertheless, there is still a lack of methods which would allow for rapid and sensitive evaluation of tumor cell growth to facilitate high-throughput screening of drugs in vivo. In our bioluminescent zebrafish transplantation model, we proposed and validated a new screening platform for pre-clinical drug discovery in zebrafish embryos. In our experiments we used the NanoLuc luciferase, which enabled us to rapidly screen inhibitors of cancer growth in a sensitive and quantitative way with very low background compared to the conventional fluorescence signal. In our screen we evaluated the in vivo drug response of 180 kinase inhibitors in zebrafish embryos...
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The urgent medicine at the military training and in the foreign misions
Bourková, Kateřina ; Bartůněk, Petr (advisor) ; Kotík, Ilja (referee)
The main goal was inform civil medics about usage acute (immediate) medicive in our military mediacal support and battle and placekeeping mision. In front line Dressing station Role -1 the medics can affect surfoval and recovery of soldier that was hurt or sich by thein tasks and knowledges. The work was dividend Inko five chapters. The introductory part is focused on history of military medicine, her posihilities use in presents and mainly in missions. The charter is aids of basic account of comformities and differences in acute medicine and in disaster medicine. In others charter is naped basic strukture of military medicine with action statement of garrison infirmaries. The lost part of theoretic is fully focused on aktivity Role 1 in Kosovo. Praktical (observational) port of diploma work contains light casustries. They adds theoretical ports. These casustries passed of during my working in mission (KFOR-Kosovo Force, Kosovo 2007) and on army training two. At the end is publicated one vez intrested casuistry from field-hospital in Afganistan. There is the nost important treament of dog-flea encefalitis end akut pankreatitis
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The Management of Patients in the Vegetative State
Nettlová, Marie ; Bartůněk, Petr (advisor) ; Drábková, Jarmila (referee)
The goal of this thesis is in picturing the problems of care about the patients in the Vegetative State in intensive care units at Prague and Region of Central Bohemia. The thesis shall reveal fundamental informations about the number of patients in the Vegetative State, to take of basal stimulation in nursing care, cooperating with the family and assess the consciousness of these patients. The theoretical part is devoted to description of the Vegetative State, basal stimulation, reaction of family, needs of family members, basic examination of consciousness. The research part is heading towards the goal of this thesis a survey based verification of the lied out hypothesis. Research was realized from December 2008 till February 2009. The list of questions was intended for sister wards at intensive care units. The outcomes are summarized in the discussion and the final part of the thesis. The average rate of occurrence these patients is higher then in published foreign studies. The result of this thesis is not acceptable for representative and applicable exoteric health care institutions.
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Mapping of regulatory elements within 5' region of the Disp3 locus
Oltová, Jana ; Bartůněk, Petr (advisor) ; Krásný, Libor (referee)
Dispatched 3 (Disp3), a thyroid hormone-regulated gene, is studied extensively in our laboratory. Phenotype of cells with overexpressed Disp3 and its expression pattern make it a perfect candidate for a molecular link between thyroid hormone action and cholesterol homeostasis in the brain. Moreover, we hypothesize that it might play a role in certain neurodegenerative disorders and brain tumours. This thesis is aimed at the process of regulation of this gene via thyroid hormone receptor (TR), specifically identification of responsive elements of the thyroid hormone receptor that are necessary for the regulation. Also, we searched for elements recognized by liver X receptor (LXR), as LXR binds to the same arrangement of repeats as TR and there are a number of genes regulated by both of them. We combined in silico analysis of the Disp3 locus with reporter luciferase assays. A cluster of six elements identified around the first exon with two of them being conserved among human and mice draw our attention. In order to analyze this sequence in more detail, reporter vectors of various truncations of 3 kb region around exon 1 were constructed and tested in reporter assays. Reporter assays did not reveal any substantial element activated by TR or LXR; on the other hand, region containing repressor element(s)...
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Cloning, expression and characterization of recombinant growth factors
Svoboda, Ondřej ; Bartůněk, Petr (advisor) ; Živný, Jan (referee)
Cloning, expression and characterization of recombinant growth factors The role of growth factors and cytokines to regulate a variety of biological processes is ever increasing. Many studies have illustrated that this diverse group of proteins are capable of stimulating cellular growth, proliferation, survival, and cellular differentiation. This thesis mainly focuses on the cytokine interleukin-6 and its specific receptor (IL-6R). The IL-6R protein has been shown to exist as both a membrane-bound and soluble form. The soluble form of IL-6R (sIL-6R) signals via a mechanism called "transsignaling" that involves the receptor associating extracellularly with its ligand IL-6 to generate an IL-6/sIL- 6R agonistic complex. This complex subsequently binds directly to another IL-6 receptor, gp130 that in turn activates it to augment a specific response. It is known that the formation of the IL-6/sIL-6R complex can be greatly enhanced by converting it into a single molecule, called Hyper-IL-6. This fusion protein consists of a sIL- 6R located at the N-terminus and IL-6 at the C-terminus separated by a flexible linker sequence. It was more than ten years since human Hyper-IL-6 was first described and since then, this molecule has proven to be an ...
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Intensive Care of the Paediatric Invasive Meningococal Infection
Šutoriková, Veronika ; Vobruba, Václav (advisor) ; Bartůněk, Petr (referee)
The thesis deals with the issue of the pediatrie invasive meningococcal infection. Theoretical part is focused on the deseription of meningococcal infection, its incidence, epidemiology, clinical picture, complication and consequences. diagnosis, therapeutic and preventions procedures. Also there is an emphasis on early detection and prompt treatment of sepsis shock. The nursing section of the thesis includes comprehensive care of a seven-month-oldinfant. This is a deseriptive report which deseribes the dynamic course of the disease, aggressive treatment including antibiotics, steroids, respiratory support, circulation support, renal replacement therapy and overall therapy of disseminative intravascular coagulation syndrome. Through that care the child survived without any mutilation. The case report focused on the needs of the child and its mother in nursing care. It elaborates the main nursing diagnoses, their targets, plans and realizations.
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Regulation of gene expression by thyroid hormone receptors
Oltová, Jana ; Bartůněk, Petr (advisor) ; Fafílek, Bohumil (referee)
Hormones coordinate various processes in living organisms and their action is mediated either by cell membrane receptors or nuclear receptors. Thyroid hormones (THs) have prominent effects on the growth, development, and many aspects of metabolism, embryogenesis and early life. Their receptors (TRs) belong to the nuclear receptor superfamily, whose members function as ligand-activated transcription factors, and depending on the context, they can act both as activators or as inhibitors of transcription. Thyroid hormone receptors are encoded by two genes, TRα and TRβ, and by alternative splicing multiple isoforms are generated. The major form of TR binds to T3-response element as a heterodimer with retinoid X receptor (RXR). TRs are able to bind T3-response elements (TREs) independently of ligand occupancy. TREs contain two or more subsequent half-site sequences of AGGTCA, which are usually arranged as direct repeats with four nucleotide spacing (DR4). TH-responsive target genes are involved in a wide range of cellular pathways. Example of such gene is Disp3, which might link TH and cholesterol metabolism in certain cell types.
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