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Ovarian carcinomas - the influence of the Rho kinase family on cell migration
Barešová, Veronika ; Urbánková Rathouská, Jana (advisor) ; Fikrová, Petra (referee)
Author: Veronika Barešová Title: Ovarian carcinomas - the influence of Rho family kinases on cell migration Diploma thesis Charles University, Faculty of Pharmacy in Hradec Králové Department of Biological and Medical Sciences Ovarian carcinomas are the deadliest group of gynecological malignancies. This is due to a high rate of metastasis and acquired drug resistance. Metastasis is characterized by a phenotypic change of cells from epithelial to mesenchymal, resulting in the loss of cell-cell and cell-extracellular matrix contacts and subsequent cell movement. This process is called epithelial-mesenchymal transition and has been shown to also play a role in cell resistance to cisplatin. The movement of individual cells is mediated by the Rho GTPase family and can be divided into mesenchymal and amoeboid types. The amoeboid type is characterized by the Rho/ROCK signaling pathway, while mesenchymal movement is mediated by the Rac/Cdc42 pathways. In my thesis, we investigated the differences in expression of selected proteins in both signaling pathways in ovarian carcinoma cells. Initially, we focused on comparing the A2780 and A2780cis cell lines, which are resistant to cisplatin. We monitored markers of amoeboid movement, RhoA and phosphorylated myosin, and markers of mesenchymal movement, Wave-2,...
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Ovarian carcinomas - the influence of the Rho kinase family on cell migration
Barešová, Veronika ; Rathouská, Jana (advisor) ; Fikrová, Petra (referee)
Author: Veronika Barešová Title: Ovarian carcinomas - the influence of Rho family kinases on cell migration Diploma thesis Charles University, Faculty of Pharmacy in Hradec Králové Department of Biological and Medical Sciences Ovarian carcinomas are the deadliest group of gynecological malignancies. This is due to a high rate of metastasis and acquired drug resistance. Metastasis is characterized by a phenotypic change of cells from epithelial to mesenchymal, resulting in the loss of cell-cell and cell-extracellular matrix contacts and subsequent cell movement. This process is called epithelial-mesenchymal transition and has been shown to also play a role in cell resistance to cisplatin. The movement of individual cells is mediated by the Rho GTPase family and can be divided into mesenchymal and amoeboid types. The amoeboid type is characterized by the Rho/ROCK signaling pathway, while mesenchymal movement is mediated by the Rac/Cdc42 pathways. In my thesis, we investigated the differences in expression of selected proteins in both signaling pathways in ovarian carcinoma cells. Initially, we focused on comparing the A2780 and A2780cis cell lines, which are resistant to cisplatin. We monitored markers of amoeboid movement, RhoA and phosphorylated myosin, and markers of mesenchymal movement, Wave-2,...
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Specific aspects of 20th century artwork of educational programme in gallery
Krechler Barešová, Veronika ; Bláha, Jaroslav (advisor) ; Kitzbergerová, Leonora (referee)
The thesis focuses on difference between theoretical and didactical aspects of work with artwork of so-called great styles (from the Romance art until art of 19. century), modern and postmodern artefact. It surveys the current thought of the early 21st century about art and visual culture. On the basis of this thought educational programs for gallery space come up. Within the framework of the thesis, two confrontational gallery educational programs have been proposed. At the end of the thesis, based on the comparison of these programs the specific aspects of 20th century artwork of educational programme in gallery have been formulated.
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Specific aspects of 20th century artwork of educational programme in gallery
Krechler Barešová, Veronika ; Bláha, Jaroslav (advisor) ; Kitzbergerová, Leonora (referee)
The thesis focuses on difference between theoretical and didactical aspects of work with artwork of so-called great styles (from the Romance art until art of 19. century), modern and postmodern artefact. It surveys the current thought of the early 21st century about art and visual culture. On the basis of this thought educational programs for gallery space come up. Within the framework of the thesis, two confrontational gallery educational programs have been proposed. At the end of the thesis, based on the comparison of these programs the specific aspects of 20th century artwork of educational programme in gallery have been formulated.
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