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The impact of unilateral load on violinists' and violists' posture
Balcarová, Barbora ; Raška, Otakar (advisor) ; Grünerová Lippertová, Marcela (referee)
The main goal of this study is to discuss the impact of unilateral load on violinists' and violists' posture. Moreover, we intended to describe physical problems amongst musicians. Methods: Subjects were recruited through email communication with orchestras and an advert posted on social media. The subjects were divided into two groups. The first group, called the musicians (H), included 30 violinists (21) and violists (9) in total. The second group was composed of healthy university students who were not exposed to unilateral loads. The process of data collection was split into two parts. Firstly, the subject was acquainted with the goal of the study and with the process of data collection, also the permissions form was signed. In the first part, Moire topography method was used. Both groups were tested in the standing position. The group of musicians was also tested in the playing position with the instrument in standing. Moreover, a specific form was filled focusing on pain, playing habits and ergonomics. Results: The comparison of the standing position between the musicians and control group observed on DIERS formetric 4D did not show any significant divergence within sagittal imbalance, coronal imbalance, kyphotic angle, lorodotic angle and scoliotic angle. Futhermore, the standing position and the...
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Synthesis and separation of arsenic-glutathione complexes
Balcárová, Barbora ; Petry-Podgórska, Inga (advisor) ; Červený, Václav (referee)
The arsenic-glutathione complexes are very unstable in solution and tend to decompose during separation in liquid chromatography. The aim of this work was to develop a relatively fast method of the synthesis and storage conditions for the arsenic-glutathione complexes. The thesis is focused on synthesis, stability in-solution and separation of arsenicglutathione complexes. The synthesis was carried out in solution of 2 mM TCEP (tris(2carboxyethyl)phosphine) in water and with excess of the glutathione. Solutions of 20 ppb arsenic-complexes were consecutively measured after 1h, 2h, 3h, 4h and 24 hours of synthesis. The results confirmed stability of the arsenic-complexes in the reaction mixtures over 24 h. The arsenic-glutathione complexes were separated using a reversed phase high performance liquid chromatography (RP-HPLC) coupled with inductively coupled plasma - mass spectrometry (ICP-MS). The chromatographic method was developed using Aeris widepore 3.6u XB-C18 250x2.10mm column. Isocratic and gradient elutions were compared using several compositions of mobile phases and time of the separation. Methods were tested using samples of synthesized arsenic-glutathione complex (DMAs(GS)). An application of the isocratic elution enabled elimination of time needed for the separation and conditioning of...
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