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Evaluation of phenolic compounds content in fruits
Baďurová, Kristýna ; Sklenářová, Hana (advisor) ; Šatínský, Dalibor (referee)
Charles University Faculty of Pharmacy in Hradci Králové Department of Analytical Chemistry Candidate: Bc. Kristýna Bortlová Supervisor: Doc. PharmDr. Hana Sklenářová, Ph.D. Title of Diploma Thesis: Evaluation of phenolic compounds content in fruits This diploma thesis is focused on the analysis of selected phenolic compounds (gallic acid, chlorogenic acid, epicatechin, rutin, quercitrin, phloridzin and phloretin). The content of these phenolic substances was evaluated in ten selected varieties of apples using an optimized HPLC method. A general description of phenolic substances is included in the thesis. Further, the thesis contains a short review of scientific publications devoted to the content of phenolic substances in fruits and a short description of the HPLC method for determination of phenolic substances. HPLC analysis was performed on the guard column Ascentis Express C18 (5 x 4.6 mm x 5 μm), and separation columns Kinetex C18 (150 x 4.6 mm x 5 μm) with core- shell particles and Luna Omega Polar C18 column (150 x 4.6 mm x 5 μm) with fully porous particles. Detection was performed by a UV detector at wavelengths of 255, 280, 320 and 365 nm. The injected volume was 10 μl and a flow rate of 1 ml/min at column temperature of 30 řC was used. A gradient elution of the mobile phase was applied...
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Evaluation of phenolic compounds content in fruits
Baďurová, Kristýna ; Sklenářová, Hana (advisor) ; Šatínský, Dalibor (referee)
Charles University Faculty of Pharmacy in Hradci Králové Department of Analytical Chemistry Candidate: Bc. Kristýna Bortlová Supervisor: Doc. PharmDr. Hana Sklenářová, Ph.D. Title of Diploma Thesis: Evaluation of phenolic compounds content in fruits This diploma thesis is focused on the analysis of selected phenolic compounds (gallic acid, chlorogenic acid, epicatechin, rutin, quercitrin, phloridzin and phloretin). The content of these phenolic substances was evaluated in ten selected varieties of apples using an optimized HPLC method. A general description of phenolic substances is included in the thesis. Further, the thesis contains a short review of scientific publications devoted to the content of phenolic substances in fruits and a short description of the HPLC method for determination of phenolic substances. HPLC analysis was performed on the guard column Ascentis Express C18 (5 x 4.6 mm x 5 μm), and separation columns Kinetex C18 (150 x 4.6 mm x 5 μm) with core- shell particles and Luna Omega Polar C18 column (150 x 4.6 mm x 5 μm) with fully porous particles. Detection was performed by a UV detector at wavelengths of 255, 280, 320 and 365 nm. The injected volume was 10 μl and a flow rate of 1 ml/min at column temperature of 30 řC was used. A gradient elution of the mobile phase was applied...
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