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Histological analysis of selected markers in cardiometabolic diseases 1
Bůtorová, Kateřina ; Vitverová, Barbora (advisor) ; Němečková, Ivana (referee)
Non-alcoholic fatty liver disease (NAFLD) is a condition characterized by the accumulation of excess fat in the liver that is not accompanied by excessive alcohol consumption. In the first stage, there is a simple fatty liver (steatosis) due to excessive fat deposition in the liver cells. As the disease progresses, there is the development of an inflammatory response and the transition to non-alcoholic steatohepatitis. The aim of this study was to evaluate whether there is the development of an inflammatory response and endothelial dysfunction after administration of a CDAA diet. To clarify this hypothesis, the analysis of VCAM-1, ICAM-1, and endoglin expression was selected. In this study, male C57BL/6 mouse model was used. The control group was fed a standard laboratory diet, and the experimental group received the CDAA diet to induce NAFLD for 4 weeks. Administration of the CDAA diet reduces the oxidation of fatty acids in hepatocytes, increases lipid accumulation, induces oxidative stress, and causes steatosis and the development of inflammation in liver tissue. The expression of selected markers of inflammation and endothelial dysfunction was detected using indirect immunohistochemistry. The results of this study showed a significant difference in the expression of the proinflammatory VCAM-1...
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