National Repository of Grey Literature 57 records found  1 - 10nextend  jump to record: Search took 0.01 seconds. 
Comparison of in vitro methods for the study of cytotoxicity
Eliášová, Pavla ; Maixnerová, Jana (advisor) ; Bárta, Pavel (referee)
Charles University Faculty of Pharmacy in Hradec Králové Department of Pharmacology and Toxicology Student: Pavla Eliášová Supervisor: RNDr. Jana Maixnerová, Ph.D. Title of diploma thesis: Comparison of in vitro methods for the study of cytotoxicity People are exposed to a growing number of toxic substances from the environment. Endocrine disrupting chemicals (EDCs) are a broad category of molecules that are thought to cause adverse effects on the endocrine system by interfering with the synthesis, transport, degradation or action of endogenous ligands. One of the aims of this thesis was to determine the in vitro toxicity of 17 selected endocrine disruptors on the human hepatocellular carcinoma HepG2 cell line. Cell viability was determined using the CellTiter96® AQueous One Solution Cell Proliferation Assay colorimetric method, the principle of which is the reduction of MTS to the colored product formazan by mitochondria in viable cells. The cytotoxic potential of the compounds was expressed by using the toxicological parameter IC50, which was measured in three time intervals (6, 12 and 24 hours). For 14 substances: Atrazine, DHEP, Bisphenol A, Carbofuran, 3-hydroxycarbofuran, Cypermethrin, DDE, DES, MEHP, PCB 118, PCB 153, PFOA, PFOS, Propiconazole, IC50 > 100 µM (respectively > 250 µM) was...
In vitro saturation study of radiolabelled monoclonal antibody carotuximab
Sladká, Anežka ; Bárta, Pavel (advisor) ; Nový, Zbyněk (referee)
v anglickém jazyce Charles University Faculty of Pharmacy in Hradec Králové Department of Biophysics and Physical Chemistry Student: Anežka Sladká Supervisor: Mgr. Pavel Bárta, Ph.D. Thesis title: In vitro saturation study of radiolabeled monoclonal antibody carotuximab Angiogenesis, or the formation of new blood vessels from existing ones, is currently one of the processes targeted by cancer therapy, as the formation of new blood vessels is a prerequisite for successful tumor growth and eventual expansion in the body in the form of metastasis. One of the receptors involved in signalling leading to angiogenesis is endoglin (CD105). This receptor is also targeted by the chimeric monoclonal antibody carotuximab. The aim of this thesis was to perform indirect labelling of the monoclonal antibody carotuximab with the radionuclide gallium-67 using the bifunctional chelator 1,4,7-triazacyclononane,1-glutaric acid-4,7-acetic acid (NODAGA). During the labelling process, the NODAGA:carotuximab molar ratio was optimized (1:20, 1:50 and 1:100). The prepared radiopharmaceutical [67 Ga]Ga-NODAGA-carotuximab was subsequently determined for its radiochemical purity by HPLC with radiometric detection. This was followed by in vitro binding experiments on human renal tumor cell culture involving internalization,...
The presentation of Lathyrus pratensis L. in different types of grass stands
BÁRTA, Pavel
In the years 2007 to 2008 the occurence and domination of the meadow peavine was studied in the foothills of Sumava (Kaplice-Velký Chuchelec and Rožnov) in the elevation of 580-650 m above sea level and in the area of Českobudějovická pánev (Kozina u Závrat in the elevation of 390-510 m). The use of the meadow peavine in various types of areas and diverse methods of employment and maintenance were studied. Also performed was the analysis of environmental conditions, phytocenological analysis of grass areas and the evaluation of employment of the meadow peavine in various typologicaly different grass areas. In the locality of Velký Chuchelec there were taken pods with seeds of meadow peavine which, after proper shelling and drying, were subject to analysis of germination in containers for experiments in regards to possibilities of introducing the meadow peavine into fodder crops. In the year 2007 to 2008, in the area of Kozina u Závrat the assesment of the occurence and degree of assault of the meadow peavine with regards to the downgrade in the quality of the fodder of this species during the assault of this patogen was studied.
In vitro saturation study of gallium-67 and zirconium-89 labelled monoclonal antibody ramucirumab on SKOV-3 cell line
Holodňáková, Nikola ; Bárta, Pavel (advisor) ; Smutná, Lucie (referee)
Charles University Faculty of Pharmacy in Hradec Králové Department of Biophysics and Physical Chemistry Student: Nikola Holodňáková Supervisor: Mgr. Pavel Bárta, Ph.D. Name of the work: In vitro saturation study of gallium-67 and zirconium-89 labelled monoclonal antibody ramucirumab on SKOV-3 cell line. Targeted biological treatment becomes more and more important with the development of a new therapy in oncology. It stimulates immune system to eliminate cancer cells. Significant progress has been made since the introduction of monoclonal antibodies. They represent one of the newest possibility used in diagnosis and treatment of tumours. The ability of the monoclonal antibody ramucirumab is to recognize and bind specifically to tumour cell antigens such as the VEGF type 2 receptor (VEGFR-2) and thus to supress angiogenic process. Anti-angiogenic ramucirumab inhibits this receptor via blocking of VEGF binding sites, which prevents the growth of tumours. It is possible to increase antitumor effect of monoclonal antibodies by their combination with other molecules like radionuclides, toxins and cytostatics when forming the so called conjugates. Prepared immunoconjugates serve as diagnostic and therapeutic tools also in Nuclear Medicine. The aim of the experimental work in was the preparation of...
Determination of toxicity using a cell model.
Fojkesová, Karolína ; Maixnerová, Jana (advisor) ; Bárta, Pavel (referee)
Charles University Faculty of Pharmacy in Hradec Králové Department of Pharmacology and Toxicology Student: Karolína Fojkesová Supervisor: RNDr. Jana Maixnerová, Ph.D. Title of diploma thesis: Determination of toxicity using a cell model The subject of the diploma thesis was measure and evaluate the cytotoxicity in vitro of 22 newly synthesized substances with potential use for their antifungal, antibacterial and antimycobacterial activity on the standard HepG2 cell line. The tested substances were synthetized at the Department of Organic and Bioorganic Chemistry at the Faculty of Pharmacy in Hradec Králové, Charles University and their chemical structure is mafenide derivative. The compounds are MAF, MAF-4, MAF-8, MAF-9, MAF-10, MAF-11, MAF-12, MAF-13, MAF- 14, MAF-16, A-5-F, A-3,5-F2, A-3-Br-5-Cl, A-3,5-Cl2, A-3,5-Br2, A-5-Br, A-THIOF, A-SA, A-3,5-I2, A-5-Cl, A-3-I-5-Cl, A-5-I. Cytotoxicity has been measured using the CellTiter 96® AQueous One Solution Cell Proliferation Assay, which is a colorimetric method based on the reduction of the tetrazolium salt of MTS to the colored soluble formazan, and substances have been compared and evaluated according to the IC50 parameter. In case of 9 substances, specifically MAF, MAF-8, MAF-11, MAF-12, MAF-16, A-5- F, A-3,5-F2, A-SA, A-5-Cl, the IC50 was greater than...
Study of renal transport mechanisms of radiolabeled antibodies
Karasová, Jana ; Trejtnar, František (advisor) ; Bárta, Pavel (referee)
Charles University in Prague, Faculty of Pharmacy in Hradec Kralove Department: Department of Pharmacology and Toxicology Candidate: Mgr. Jana Kanokova Supervisor: Doc. PharmDr. Frantisek Trejtnar, CSc. Title of rigorous thesis: Study of renal transport mechanisms of radiolabeled antibodies Utilization of monoclonal antibodies belongs to one of the important strategies in the treatment of oncological diseases. This treatment has a great advantage in precise focusing on the target structure with minimal damage to surrounding non-target tissue. The antibodies against the receptor for epidermal growth factor (EGFR) expressed in higher rate by variety of tumor types represent one of the important group. Nowadays oncology utilizes both unconjugated antibodies, and the ones conjugated with other effector components, e.g. cytostatic agents, radionuclides, toxins. One of the important limits for the antibodies conjugated with radionuclides appears to be their potential accumulation in kidneys with subsequent radiotoxic damage to the kidney tissue. The aim of this experimental study was to investigate the accumulation of three 131 I- labeled anti-EGFR antibodies - panitumumab, cetuximab and nimotuzumab using a kidney model in vitro, and compare it with renal uptake of the natural peptide ligand EGFR which was...
In vitro saturation study of 99mTc-HYNIC-ramucirumab on PC-3 cell line
Lach, František ; Bárta, Pavel (advisor) ; Smutná, Lucie (referee)
v anglickom jazyku Charles University in Prague Faculty of Pharmacy in Hradec Králové Department of Biophysics and Physical Chemistry Student: František Lach Supervisor: Mgr. Pavel Bárta, PhD Consultant: Mgr. Lucie Hyršová Title of diploma thesis: In vitro saturation study of 99m Tc-HYNIC-ramucirumab on PC-3 cell line The number of malignant tumours in the population has increased in recent years. Due to the frequent serious sides effects of chemotherapeutic drugs on the whole organism, targeted antitumor therapy is at the forefront. Due to its specific effect on the regulatory and signal pathways of protein structures, monoclonal antibodies are used for the target anti-tumour therapy. The basic properties of the growing tumour include vasculogenesis (the ability to build new blood vessels from the endothelial precursors) and angiogenesis (the process of self-inducing formation of blood vessels). Endothelial tumour progenitors include vascular endothelial growth factor (VEGF). VEGF activates its biological activity by binding to its transmembrane tyrosine-kinase receptors VEGFR. Indeed, the inhibition of the vascular endothelial factor receptors is the target of some monoclonal antibodies. Ramucirumab is a monoclonal antibody that selectively inhibits VEGF receptor type 2 (VEGFR-2) and thereby...
Copper chelating potential of iron chelators from the group of aroylhydrazones
Kříž, Jakub ; Mladěnka, Přemysl (advisor) ; Bárta, Pavel (referee)
Charles University in Prague, Faculty of Pharmacy in Hradec Králové Department of Farmacology and toxicology Candidate Mgr. Jakub Kříž Consultant Doc. PharmDr. Přemysl Mladěnka, Ph.D. Title of Thesis Copper chelating potential of iron chelators from the group of aroylhydrazones Copper is one of the essential trace elements. It is present in two oxidation-reduction forms Cu+ and Cu2+ in the human body. The ability of an easy transformation between this two forms is the crucial feature for the activity of copper enzymes, like superoxide dismutase and cytochrome c oxidase. Copper kinetics is quite complicated. The key organ which affects copper kinetics is the liver, but the small intestine plays an important regulatory role too. There are disorders, which are associated with both copper deficiency and accumulation. The best known disorder caused by copper excess is Wilson's disease. It is caused by a mutation in the ATP7B gene. It codes a transporter, which is responsible for elimination of copper. Excessive accumulation of copper causes several symptoms, mainly hepatic and neurological problems. Wilson's disease is treated by chelators, which bind the excess of metal ions in the organism. The aim of my thesis was to verify the copper-chelation efficiency of three selected iron chelators. The...
Study of cytotoxicity of potential antituberculotics using selected methods on liver and kidney cell line
Katrnošková, Simona ; Ramos Mandíková, Jana (advisor) ; Bárta, Pavel (referee)
Charles University Faculty of Pharmacy in Hradec Králové Department of Pharmacology and Toxicology Student: Mgr. Simona Katrnošková Supervisor: PharmDr. Jana Ramos Mandíková, Ph.D. Title of Thesis: Study of cytotoxicity of potential antituberculotics using selected methods on liver and kidney cell line During pharmacologic therapy, tissues of liver and kidney are frequently exposed to high doses of xenobiotics. Via in vitro assays during preclinical testing, we are able to predict potential toxicity which helps to prevent the possible serious adverse drug reactions in clinical practice. The aim of this rigorous thesis was to state the cytotoxic profile of 4 potential antituberculotic drug candidates using appropriate cell models and in vitro assays. Another aim was to compare the cytotoxic effect of the tested compounds among themselves and to 4-aminosalicylic acid (PAS) which antituberculotic activity is known for many years. The tested substances were 3 salicylanilide diethyl phosphate-based derivatives and 4-(trifluormethyl)benzoic acid. For the cytotoxic potential assessment, we used the parameter half maximal inhibitory concentration IC50. We have used methods determining the cell metabolic activity, aminopeptidase activity, lactate dehydrogenase leakage and activity of 3/7 caspases in this...

National Repository of Grey Literature : 57 records found   1 - 10nextend  jump to record:
See also: similar author names
8 BÁRTA, Pavel
5 BÁRTA, Petr
1 Bárta, Patrick
1 Bárta, Patrik
5 Bárta, Petr
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