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Optimization of bioethanol production by Zymomonas mobilis
Andrlová, Kateřina ; Vítová, Eva (referee) ; Babák, Libor (advisor)
Diploma thesis deals with use of Zymomonas mobilis for the production of bioethanol from waste paper. There were used three kinds of substrate (cardboard, drawing and office paper) to optimize of bioethanol production. Individual papers were subjected to the same pre treatment, namely a milling, a combination of microwave irradiation and NaOH, a combination of microwave irradiation and H2SO4 and combination microwave irradiation, H2SO4 and NaOH. The substrates were decomposed by enzymatic hydrolysis after pre treatment to evaluate the best pre-treatment. Simultaneous saccharification and fermentation was carried out for each substrate (with two of the best pre-treatment). The samples were taken during the hydrolysis and the simultaneous saccharification and fermentation, and were determined by HPLC. Growth curves of Zymomonas mobilis were constructed, as the most appropriate for SSF was chosen temperature of 40 ° C in which the exponential phase took place at the time of 6 15 hours. During hydrolysis was monitored glucose concentration in the solution. The maximum concentration of glucose was in the cardboard (microwaves + H2SO4 + NaOH) 16.46 gdm-3, a drawing (microwaves + H2SO4 + NaOH) 31.78 gdm-3, and office paper (microwaves + H2SO4) 25.04 gdm-3. The concentration of ethanol for SSF was highest in the same cases as in the hydrolysis. The cardboard was the maximum concentration of bio ethanol 9.5 gdm-3, for the drawing 16.1 gdm-3 and for the office paper 12.13 gdm-3.
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Rowanberry - nutritional characteristic and utilization in grocery
Andrlová, Kateřina ; Vítová, Eva (referee) ; Vespalcová, Milena (advisor)
Bachelor elaborate is focused on one the less used species of small fruits - rowanberries. It gives an overview of the particular biologically active substances in this fruit and the use of the fruits in the human diet. Introduction is focused on the botanical description of rowan - Sorbus. There are presented the best known species of genus rowan. The greatest attention is paid to the type of Sorbus aucuparia which provide sweet variety and nutritional value of the fresh fruits. The second part is devoted to the description of biologically active substances contained in food rowanberries. The most valuable of them are vitamin C, flavonoid compounds (quercetin, campferol), phenolic compounds (chlorogenic acid) and pectins. Possibility of processing rowanberries in a variety of products are presented in next part. They are made of these compotes, jams, candied fruits, jellies and juice. Can be used for production of wine, cider and liqueur. In conclusion, there are described methods for determination the content of selected substances rowanberries (vitamin C, pectins). There are mentioned here primarily manual methods.
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Optimization of bioethanol production by Zymomonas mobilis
Andrlová, Kateřina ; Vítová, Eva (referee) ; Babák, Libor (advisor)
Diploma thesis deals with use of Zymomonas mobilis for the production of bioethanol from waste paper. There were used three kinds of substrate (cardboard, drawing and office paper) to optimize of bioethanol production. Individual papers were subjected to the same pre treatment, namely a milling, a combination of microwave irradiation and NaOH, a combination of microwave irradiation and H2SO4 and combination microwave irradiation, H2SO4 and NaOH. The substrates were decomposed by enzymatic hydrolysis after pre treatment to evaluate the best pre-treatment. Simultaneous saccharification and fermentation was carried out for each substrate (with two of the best pre-treatment). The samples were taken during the hydrolysis and the simultaneous saccharification and fermentation, and were determined by HPLC. Growth curves of Zymomonas mobilis were constructed, as the most appropriate for SSF was chosen temperature of 40 ° C in which the exponential phase took place at the time of 6 15 hours. During hydrolysis was monitored glucose concentration in the solution. The maximum concentration of glucose was in the cardboard (microwaves + H2SO4 + NaOH) 16.46 gdm-3, a drawing (microwaves + H2SO4 + NaOH) 31.78 gdm-3, and office paper (microwaves + H2SO4) 25.04 gdm-3. The concentration of ethanol for SSF was highest in the same cases as in the hydrolysis. The cardboard was the maximum concentration of bio ethanol 9.5 gdm-3, for the drawing 16.1 gdm-3 and for the office paper 12.13 gdm-3.
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Rowanberry - nutritional characteristic and utilization in grocery
Andrlová, Kateřina ; Vítová, Eva (referee) ; Vespalcová, Milena (advisor)
Bachelor elaborate is focused on one the less used species of small fruits - rowanberries. It gives an overview of the particular biologically active substances in this fruit and the use of the fruits in the human diet. Introduction is focused on the botanical description of rowan - Sorbus. There are presented the best known species of genus rowan. The greatest attention is paid to the type of Sorbus aucuparia which provide sweet variety and nutritional value of the fresh fruits. The second part is devoted to the description of biologically active substances contained in food rowanberries. The most valuable of them are vitamin C, flavonoid compounds (quercetin, campferol), phenolic compounds (chlorogenic acid) and pectins. Possibility of processing rowanberries in a variety of products are presented in next part. They are made of these compotes, jams, candied fruits, jellies and juice. Can be used for production of wine, cider and liqueur. In conclusion, there are described methods for determination the content of selected substances rowanberries (vitamin C, pectins). There are mentioned here primarily manual methods.
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Communication Strategy of Selected Company
Andrlová, Kateřina ; Zeman, Jiří (advisor) ; Filipová, Alena (referee)
This Bachelor thesis is dedicated on communication strategy of company Ford MotoTrade VM and its goal is an evaluation of this strategy. In the introduction to the theoretical part I occupy with marketing, its definition and brief history. The separate sub-chapter is devoted to marketing mix as the key issue of this work. At the end of first chapter SWOT analysis is described. The practical part is then focused on the company Ford MotoTrade VM itself, mainly focused on the description of her used communication strategy. There are presented results of a survey. At the end of the chapter there are my recommendations based on theoretical knowledge, the survey results and SWOT analysis.
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