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Use of cytogenetical methods in taxonomy of Arachnida (Arachnida)
Alaverdyan, Argam ; Šťáhlavský, František (advisor) ; Johnson Pokorná, Martina (referee)
This bachelor thesis deals with the use of cytogenetic methods in taxonomy of Arachnida. To understand this matter, we need to analyse available information about inter and intraspecific karyotype variability of individual orders. At the beginning of the thesis there is a description of cytogenetic methods used with Arachnida.They are used to gather the karyotype's fundamental information, such as the diploid number of chromosomes, chromosome morfology and possible occurence of sex chromosomes. Main part of the thesis is focused on describing karyotypes of selected orders (Amblypygi, Palpigradi, Pseudoscorpiones, Scorpions, Opiliones and Araneae) and considering the convenience of use of cytogenetic methods for their taxonomy based on these information. The thesis includes current number of described genera (species) of the orders, as well as a number of cytogenetically analyzed genera (species) for comparison.
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Karyotype variability of harvestmen from Nemastomatidae family (Arachnida: Opiliones)
Alaverdyan, Argam ; Šťáhlavský, František (advisor) ; Symonová, Radka (referee)
This master's thesis is focused on cytogenetic analysis and karyotype variability of the Nemastomatidae family. This family comprises morphologically uniform harvestmen of small sizes, with low mobility, and with center of distribution in Europe. Karyotype differences could play an important role for detection of cryptic diversity in this family. The karyotype analysis is focused mainly on Alpine and Pyrenean endemic species but also on other taxons located in Central Europe. The goal was not only to identify the differences which occur between the specific genera and species, but also eventually between populations. For detection of the specific chromosomal alterations in evolution of the karyotype in Nemastomatidae the fluorescent in situ hybridization (FISH) was used, localizing the positions and amounts of gene clusters for 18S rRNA. From the results we can assume that the number of chromosomes in the family Nemastomatidae can range between 2n = 12- 30. Further it was found out that in Nemastomatidae the biarmed chromosomes are more prevalent, and that the species which have lower amounts of chromosomes contain chromosomes that noticeably differ in size (probably because of chromosomal fusions). These results indicate that with some morphologically uniform species, the knowledge of specific...
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Use of cytogenetical methods in taxonomy of Arachnida (Arachnida)
Alaverdyan, Argam ; Šťáhlavský, František (advisor) ; Johnson Pokorná, Martina (referee)
This bachelor thesis deals with the use of cytogenetic methods in taxonomy of Arachnida. To understand this matter, we need to analyse available information about inter and intraspecific karyotype variability of individual orders. At the beginning of the thesis there is a description of cytogenetic methods used with Arachnida.They are used to gather the karyotype's fundamental information, such as the diploid number of chromosomes, chromosome morfology and possible occurence of sex chromosomes. Main part of the thesis is focused on describing karyotypes of selected orders (Amblypygi, Palpigradi, Pseudoscorpiones, Scorpions, Opiliones and Araneae) and considering the convenience of use of cytogenetic methods for their taxonomy based on these information. The thesis includes current number of described genera (species) of the orders, as well as a number of cytogenetically analyzed genera (species) for comparison.
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