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Methodology for measuring the character of the mucus layer in fish
Halačka, Karel
The aim of the methodology is to enable easy quantification of selected characteristics of the fish integument, namely the number of secretory cells in the surface layer of the skin, the amount of epidermal melanophores and the ability of the mucus layer to absorb UV radiation. The target group of the methodology are mainly persons from veterinary practice and ichthyologists dealing with the issue of fish integument, in relation to their health status or developmental-morphological studies. It can also be used in fish farming or protection. Representatives of different taxonomic groups were preferred in the selection of fish species for verification.
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Mucus collection in fish
Halačka, Karel ; Mareš, J.
The aim of the methodology is to acquaint the professional public and users with the information obtained and the evaluation of basic methods of mucus collection in fish. Based on the knowledge and evaluation, to enable the most efficient collection of material for subsequent research. The target group of the methodology are mainly persons from veterinary practice and ichthyologists dealing with the issue of fish integument, both in relation to their health status or developmental-anatomical studies. Representatives of different taxonomic groups were preferred in the selection of fish species for verification.
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Influence of sampling and fixation on measuring of genome size
Halačka, Karel ; Vetešník, Lukáš ; Fedorčák, J. ; Koščo, J.
Assessment of genome size is an important aspect of many modern studies on fish. Blood is the most common DNA source in fish studies due to its relative ease of sampling and processing. Fish blood, however, precipitates rapidly, leading to clotting. Consequently, anticoagulants (primarily heparin) are added at the time of sampling. Using of increasing heparin concentrations resulted in notable increases in fish erythrocyte genome size readings compared to a non-heparinised control. The differences observed were highly significant, suggesting that the results of previous comparative studies or those determining individual polyploidy may need reinterpretation.
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DNA servis a konkrétní genetické produkty pro chovatele nejen lososovitých ryb
Mendel, Jan ; Mareš, J. ; Palíková, M. ; Halačka, Karel ; Vetešník, Lukáš
Mnohaleté zkušenosti s poskytováním genetického servisu a s řešením projektů spojených s chovem akvakulturních ryb a ochranářským managementem ryb ve volných vodách vedl vědecko-výzkumný tým k přípravě genetického toolboxu pro chovatele, rybáře a ochranáře. Tři připravené sady - "Standard", "Salmonid" a "Rezistence" poskytují nástroje a postupy řešící tematické oblasti: identifikační genetiky, reprofish genetiky, geneticky kontrolovaného chovného managementu a imunogenetiky včetně NGS technik. Nabízené postupy a řešení vycházejí ze vzájemné spolupráce univerzit, výzkumných institucí, chovatelských firem, rybářských sdružení a ochranářských organizací. Konkrétněji především z národního a evropského patentu a certifikovaných metodik připravených v Ústavu biologie obratlovců (ÚBO), Ústavu zoologie, rybářství, hydrobiologie a včelařství AF MENDELU a Ústavu ekologie a chorob zvěře, ryb a včel FVHE VFU.
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Preparation and storage of fish samples for flow cytometry
Halačka, Karel ; Vetešník, Lukáš ; Mareš, J.
Flow cytometry enabling rapid measurement of cellular DNA content has been widely used in ichthyology and fishery practice. The aim of the methodology is to contribute to optimization of work on the flow cytometer by helping with the selection, sampling and possibilities of fixation of analyzed tissue and interpretation of obtained data. The target group are people from fishery companies and ichthyologists dealing with the problem of the fish population structure in free water. During the sampling and analyzing of samples problem can be limited availability of fixative and techniques as well as analyzes of material not originally retained for the purpose of ploidy determination. Typical examples are temporary usable or archive preparations, museum material, etc. For the verification were preferred representatives of polyploid complexes or economically exploited species with a known existence of polyploid individuals.
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