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National Repository of Grey Literature 1 records found  Search took 0.00 seconds. 
The Occurence Of Bla(Tem) And Tet(W) Genes In Czech Wastewaters And Their Fate In Fungal-Based Wastewater Treatment Processes
 Svobodová, Kateřina ; Adámek, M. ; Petráčková, Denisa
Antibiotic resistant bacteria and genes (ARB&Gs) have presently emerged as serious environmental pollutants with a high health risk. This study analyzed the occurrence of ARGs tet(W), bla(TEM) and intl1 in microbiomes from 4 Czech wastewater treatment plants showing that bla(TEM) displayed slightly higher gene abundance compared to tet(W). Next, simulated wastewater seeded with activated sludge bacteria was treated with Pleurotus ostreatus-based bioreactor to evaluate the effect of the fungal filter on bacterial community structure and bacterial resistome in wastewaters. The results showed that functional diversity of bacterial communities was significantly changed by their contact with fungal biofilm as revealed by EcoPlate assays and analyses of bacterial 16S rDNA by denaturing gradient gel electrophoresis. A significant decrease in tet(W) abundance (48 times) was observed in fungal-treated wastewater compared to untreated one. The abundance of bla(TEM) genes increased 4 times during the adaptation of bacteria to nutrient conditions and remained unaffected by the fungal treatment. To our knowledge this work is first to study the fate of ARGs in wastewaters during their advanced biological treatment. It documented that P. ostreatus has a potential to lower the abundance of some ARGs in wastewaters.
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