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National Repository of Grey Literature

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Effect of H- and O-termination of nanocrystalline diamond films on cell adhesion and osteogenic differentiation Lišková, Jana ; Babchenko, Oleg ; Varga, Marián ; Kromka, Alexander ; Hadraba, Daniel ; Švindrych, Zdeněk ; Bačáková, Lucie In order to control the hydrophobic or hydrophilic character, the NCD film surfaces were grafted by hydrogen atoms (H-termination) or by oxygen atoms (O-termination). Human mesenchymal stem cells and primary human osteoblasts were used for biological studies on H- and O-terminated NCD films. We found that cells cultivated on O-terminated NCD films exhibit better adhesion than H-terminated NCD films. In addition, the expression of osteogenic cell markers collagen and alkaline phosphatase analysed by Realtime PCR and immunostaining was higher on O-terminated films. Detailed record

Interpretation of the C=O band of modified diamond nanoparticles by means of DFT theory Jirásek, Vít ; Kozak, Halyna ; Remeš, Zdeněk ; Kromka, Alexander Diamond nanoparticles (DNPs) are an interesting class of nanomaterials, which has many unique properties. Their applications in medicine and molecular biology have been mentioned. The DNPs were reviewed in sense of their surface chemistry and a range of analytic techniques was pointed out. The experimental analysis can be enhanced by theoretical models based on the first principles. An approach to theoretical analysis of infrared spectra was introduced. As an example, a part of FTIR spectra of the oxidized DNPs was interpreted by means of the DFT theory. The calculation of the C=O stretch vibrational frequencies of carboxylic and anhydride groups on certain model clusters enabled to explain the shift and splitting of the measured spectral bands. Detailed record

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