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National Repository of Grey Literature

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	Synthesis of new potential inhibitors of 5´-nucleotidases Šimák, Ondřej ; Pachl, Petr ; Brynda, Jiří ; Rosenberg, Ivan The work deals with the synthesis of potential inhibitors of 5´-nucleotidases. The phosphonobenzylidene ring of a phosphonate nucleotide derivative was recognized as a target for proposed synthetic modifications. Substituents selected on the basis of a docking study, mostly the electronegative ones, should be suitable for the formation of additional hydrogen bonds, and thus the increased inhibition of the enzymes. Detailed record
	NANOTECHNOLOGY AND BIOMATERIALS IN MEDICINE Kubinová, Šárka ; Jendelová, Pavla ; Lesný, Petr ; Holáň, Vladimír ; Syková, Eva Our studies focus on the use of nanotechnology in cell therapy and tissue engineering for the treatment of brain and spinal cord injury, corneal defects and chronic wounds. Detailed record
	Moderní metody určování struktur proteinových krystalů a strukturní analýza RNA polymerázy Kovaľ, Tomáš ; Dohnálek, Jan ; Dušek, Michal ; Krásný, Libor Here, we present a summary of crystallization and diffraction data collection methods that are applied in the process of structure determination. Then, structural characterisation of bacterial RNA polymerase, a multisubunit protein complex performing transcription of DNA into RNA, is reviewed. Detailed record
	Analýza samčí sterility způsobené T(16,17)43H chromosomální translokací Čapková, Jana ; Homolka, David ; Ivánek, Robert ; Jansa, Petr ; Forejt, Jiří Heterozygosity for certain mouse and human chromosomal rearrangements is characterized by male-limited sterility. Our model of a male-sterile chromosomal rearrangement represents mouse T(16;17)43H (T43) chromosomal translocation. The effect of the T43H translocation on spermatogenesis was studied. We analyzed meiosis in B10.T43/+ heterozygous male mice and we have obtained direct evidence on the transcriptional silencing of unsynapsed autosomal chromatin of the translocation quadrivalent and the subsequent failure of the X chromosome transcriptional inactivation at the mid-late pachytene of spermatogenesis. Detailed record
	Microscopy 2008 Hozák, Pavel Two-day conference responding to novel trends in light and electron microscopy and their impact on researchers in the Czech Republic and Slovakia. Contributions were presented in biomedical as well as physics and material science fields. The Conference was held in a sports area in the heart of the Czech-Moravian Heights and was closed by an opulent rout with wine degustation. Detailed record
	Buňky PC12 obsahují vazebná místa pro CART Maixnerová, Jana ; Blokešová, Darja ; Matyšková, Resha ; Haugvicová, Renata ; Šloncová, Eva ; Elbert, Tomáš ; Železná, Blanka ; Maletínská, Lenka Specific binding of 125I-CART(61-102) to the rat adrenal pheochromocytoma PC12 cell line, both intact cells and cell membranes is reported. Detailed record
	Regulace syntézy ribozomů v bakteriích Krásný, Libor Rapidly growing bacteria need a high number of ribosomes to satisly the cells need for increased translation. Starving bacteria need a low number of ribosomes to limit energy expenditure.The number of ribosomes is regulated at the level of ribosomal RNA (rRNA) transcription. Detailed record
	Původ proteinů kančí semenné plazmy Maňásková, Pavla ; Kyselová, Vendula ; Tichá, M. ; Jonáková, Věra Spermadhesins were detected with their antibodies in boar epididymal fluid, but DQH and beta-microseminoprotein were not. Indirect immunofluorescence technique was employed to determine the distribution of seminal plasma proteins on tissue sections of the reproductive organs. The mRNA of the DQH was found only in seminal vesicles, not in the epididymis, and the mRNA of beta-MSP in testis, seminal vesiclas and prostate. Detailed record
	Vazebné vlastnosti proteinů kančí semenné plazmy v reprodukčním procesu Jonáková, Věra ; Maňásková, Pavla ; Liberda, J. ; Tichá, M. Boar seminal plasma proteins (spermadhesins and DQH protein)bind the sperm surface at ejaculation. binding of sperm to oviductal epithelial cells and to the glycoproteins of zona pellucida are mediated by the protein-saccharide (lectin-like)interactions.These intractions play role in the formation of sperm oviductal reservoir, in the release of capacitated sperm from the surface of oviduct and in sperm-zona pellucida interaction. Detailed record

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