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Multiinstrumental analysis of the effect of cadmium ions on selected flax cultivars
Kryštofová, O. ; Baloun, J. ; Húska, D. ; Diopan, V. ; Shestivska, V. ; Zítka, O. ; Adam, V. ; Macek, Tomáš ; Macková, M. ; Havel, L. ; Kizek, R.
Multiinstrumental analysis of the effect of cadmium ions on selected flax cultivars was performed, revealing marked differences in synthesis of protective compounds by chromatographic, spectrometric and electrochemical techniques.
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Produkce polyklonálních protilátek proti rekombinantnímu proteinu X viru bramboru
Čeřovská, Noemi ; Plchová, Helena ; Moravec, Tomáš ; Filigarová, Marie ; Dědič, P.
The gene encoding the coat protein (CP) of Potato virus X was cloned into expression vector pMPM-4A Omega. PVX CP was expressed in Escherichia coli and was used for raising rabbit polyclonal antibodies. For immunization we purified CP fractions from bacterial lysate either by simple fractionation or by excision from SDS gel. The obtained sera and antibodies were tested for the detection of PVX in laboratory hosts Nicotiana tabacum, cv. Petit Havana SR 1 and N.benthamiana and in various cultivars of natural host Solanum tuberosum by ELISA as well as by Western blots. The antibodies were specific for PVX and are usable for the detection of the broad isolates spectrum of the Potato virus X by ELISA and Western blot.
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Potato containing a transgene for the fusion protein SPI2::GFP
Navrátil, Oldřich ; Kludkiewicz, Barbara ; Vinokurov, Konstantin ; Horáčková, V. ; Sehnal, František
Plant breeding is facing a new era, the horizons of which are determined by transition from studies of the role of individual genes in biological processes to global search for the role of genome and proteome in determination of biological processes. Thegeneral aim of the center is to utilize the potential of the functional genomics and proteomics to obtain novel information and breeding material to increase the quality of agricultural products, specifically, to improve the agronomic parameters and modifying specific properties of crop plants important not just in the Czech Republic, but also in Central Europe in general. Research will focus on increasing of (i) quality of wheat and barley corn, pea seeds and potato tubers, (ii) potato and pea resistance to pests and pathogenes, and (iii) heavy metal uptake and resistance in flux for phytoremediation.
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Elektrochemické stanovení metalothioneinu v transgenním tabáku
Křížková, S. ; Diopan, V. ; Baloun, J. ; Šupálková, V. ; Shestisvka, V. ; Kotrba, P. ; Macková, Martina ; Macek, Tomáš ; Havel, L. ; Adam, V. ; Zehnálek, J. ; Kizek, R.
Inspection of CUP1 content in selected tobacco lineages using electrochemical method optimized for its selective determination was described.
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