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National Repository of Grey Literature 1 records found  Search took 0.01 seconds. 
Haplotyping of oak populations by chloroplast sequencing reveals origin and homogeneity of populations
 Vlasák, Josef ; Cvrčková, H. ; Máchová, P.
The aim of this publication is to present new method of DNA isolation from young\noak leaves that enables efficient amplification of trnD-trnT region of chloroplast\nDNA and direct sequencing of crude PCR reaction mixture. Sequence data of trnDtrnT region have been used to examine Quercus robur and Q. petraea populations variability. High discrimination power of this method is demonstrated, comparable with previously used restriction analysis of four similar chloroplast DNA regions. Variable positions in trnT-trnD chloroplast DNA fragment are mapped and their use for oak haplotyping indicated. As an example, 20 Czech Republic oak populations were analyzed and their history, geografical origin and homo/heterogeneity were revealed.
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