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Modelling of Transport Systems
Šmíd, Jiří ; Martinek, David (referee) ; Peringer, Petr (advisor)
The presented work describes both transportation and traffic flow simulation. For that purpose two different libraries were made. The first one is based on an existing library, Simlib/C++. For the traffic flow simulation a brand new library was created, using cellular automaton rules. Above all, it is designed to facilitate analysis of fundamental attributes of traffic network. Vehicles varying in size are able to find their own route plans from point where they were generated. Both libraries offer users a large amount of flexibility in specifying transport and traffic network.
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Identification DNA of Plant and Animal Species in Food by Polymerase Chain Reaction
Šmíd, Jiří ; Hanák,, Petr (referee) ; Timko,, Jozef (referee) ; Kuchta, Tomáš (advisor)
We were developing detection methods for three food allergens of plant origin. We used real-time PCR for soy detection in food oriented on gene lec, that is coding lektine specific for soy. On this target sequence were oriented PCR system with primers Le2F and Le2R and TaqMan probe Le2P. Detection limit (2,75 pg), practical detection limit (0,02 %), inclusivity and exclusivity were determined. Whole system were quantified. Real-time PCR for pistachio detection were based on primers and probe for gene COR. Detection limit (3,5 pg), practical detection limit (0,002 %), inclusivity and exclusivity were determined. For almond detection we were not succeed system, that fulfil all qualitative parametres.
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Possibilities of using modern methods in manufacturing of prototype castings
Šmíd, Jiří ; Horenský, Jiří (referee) ; Horáček, Milan (advisor)
The introduction part of the thesis focuses on the overview of rapid prototyping in foundry industry. Principles of the most important RP methods are described and the FDM method is analyzed in more detail. This method was used in the practical part for the production of wax patterns with silicone moulds. The wax patterns were used for the production of castings using the lost wax method. The result of this work is determination of dimensional changes during the whole process of casting manufacture from the drawing to the final casting.
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Development of transient transfection protocol for HEK293 EBNA1 cells
Šmíd, Jiří ; Španová, Alena (referee) ; Ševčík, Mojmír (advisor)
Recombinant proteins belong to considerable biofarmaceutics products used in biomedical research and in the treatment of human disease. Recombinant protines can be produced by stable transfection in big amount or by faster transient transfection with smaller amounts. To provide regular biological activity, it is necessary for the protein to be properly folded and post-translationally modified. As these modifications can be accurately performed only in mammalian cells, they have become the major host for complex r-protein expression. In this thesis is described transient transfection HEK 293 EBNA1 cells with linear polyethylenimines. These cells has been adapted to suspension cultivation in serum free medium. The cells were transfected with pcDNA3.1, pCI, pEBSV1, pCEP4, pEAK8 a pcDNA5/FRT/TO plasmids, everyone contained repoter gene SEAP. Concentration of SEAP in cell culture supernatants were determined in order to compare efficiencies of individual transfections. DNA:PEI ratio was another factor which was optimised and two different PEIs were compared. Highest achieved expresion was 50 mg per litre with transfection in 24 well plate when DNA:PEI ratio was 1:5. Comparison of six different plasmids give the bigest expresion pCEP4/SEAP, in well plate as well as in scaled up system.
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Diversity of iris coloration during evolution of geckos (Gekkota)
Ozerskyi, Serhii ; Brejcha, Jindřich (advisor) ; Šmíd, Jiří (referee)
Iris is an anatomical structure that performs the function of a diaphragm in the visual system of the eye. It usually occupies most of the visible part of the eye. In addition to the main function - not to let light pass by the pupil, iris can play many different roles due to the great variability of its coloration. Iris can be part of a camouflage or aposematic color, play a different role in intra- or interspecific communication. In some species, its color changes depending on age, season, biological cycles, or even quickly become marked in response to stimuli. Geckos (Gekkota) are distinguished by a particularly large variety of iris color. And in many of them, the iris pattern resembles branching veins. What significance and evolution history this pattern has is of interest. I used various models of evolutionary reconstruction in the R program to study the evolution of veined pattern of coloration of the iris, as well as phylogenetic comparative methods and binary character evolution model to identify its connections with the fusion of life and the contrast in brightness between the eye and the eye ring. It was revealed that the veined pattern is an ancestral condition for geckos and is present in most modern species. Most precisely, the evolution of the vein pattern is described by all rates different...
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Diversity and phylogeny of the lacertid lizards (Lacertidae) with emphasis on the tribe Eremiadini
Hlaváč, Denis ; Šmíd, Jiří (advisor) ; Chmelař, Jan (referee)
The family Lacertidae (lizards) belongs to the class Reptilia (reptiles) and the order Squamata (scaly reptiles). This family consists of two subfamilies, Lacertinae and Gallotinae, which are diversified into 45 genera and approximately 368 species. The subfamily Lacertinae is divided into two tribes, namely the tribus Eremiadini and Lacertini. The tribe Lacertini can be further divided into two clades: the Ethiopian and the Saharan-Eurasian clade. The subfamily Lacertinae, with approximately 354 species, is much larger than the subfamily Gallotinae, which consists of only 14 species. From the subfamily Lacertinae, the larger tribus Eremiadini is formed by 215 species classified in 22 genera. Representatives of this family can be found in Europe, Africa and Asia, but the center of origin is Europe. They belong to the dominant species of herpetofauna in Europe. The Eremiadini tribe separated from the rest of the Lacertidae family in the past and migrated to Africa, where it diversified into its present form. Currently, the diversity of the Eremiadini tribe is concentrated in Africa, but a large number of species also occur in Asia. The body is small to medium in size, which usually does not exceed 90 mm without the tail. The body shape is slender and the tail is relatively long compared to the body...
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Prevalence of Atrial Fibrillation as a Risk Factor in Patients after Circulatory Arrest for Ventricular Fibrillation
Šmíd, Jiří ; Vančura, Vlastimil (advisor) ; Mayer, Otto (referee) ; Skalická, Hana (referee)
Atrial fibrillation is the most common arrhythmia occurring in the adult population. Sudden cardiac death is one of the most common causes of cardiovascular death, accounting for up to 50% of all cardiovascular deaths. A number of trials have investigated the association between atrial fibrillation and other heart diseases, including sudden cardiac death. Atrial fibrillation is increasing in prevalence and incidence in the population and is associated with higher morbidity and mortality in patients. The pathophysiological mechanisms in atrial fibrillation are very complex and are linked to a number of other diseases. With these diseases they may share a common background and may interact together. A higher incidence of atrial fibrillation is described especially in patients with coronary artery disease, heart failure, and arterial hypertension. Increased mortality or higher incidence of ventricular arrhythmias, including ventricular fibrillation, has been described in studies in ICD carriers, but also in the general population, where atrial fibrillation has been shown to be an independent risk factor associated with up to a 3-fold increased risk of AF. This paper want to provide a comprehensive picture of the relationship between atrial fibrillation and sudden cardiac death. It provides information...
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Evolutionary dynamics of the genus Hemidactylusekkonidae) in space and time
Janák, Vojtěch ; Šmíd, Jiří (advisor) ; Rehák, Ivan (referee)
Hemidactylus is one of the most succesful groups within the infraorder Gekkota of squamate reptiles. With more than 180 species it represents the second most species-rich gekkotan genus. In last decades it has been the subject of a rather intensive research, which provided great insight into it's phylogeny, inner structure, range, biogeography and taxonomy. The number of described species has more than doubled in the same timespan. Despite this increase of knowledge as well as it's evolutionary success, which make the Hemidactylus genus a good model for study of diversification and evolution, there has been no in depth study conducted regarding these topics. This thesis constitutes an attempt at such a study of the phylogeny and evolution of the genus, it's history, dispersal and diversification dynamics, based on more complete species sampling than has been used in any study so far.
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