|
|
Design of the on-board system for a motorcycle
Ševčík, Jan ; Benešl, Lukáš (referee) ; Juráň, Radovan (advisor)
This work deals with the design and construction of an on-board computer for a vintage Jawa motorcycle. The design combines measuring sensors of our own design and those commonly available on the market, a main control board of our own design and assembly without interfering with the structure of the motorcycle. The system is considered for mounting on the Jawa 250/592 motorcycle, with some parts from other motorcycles, allowing for easier fitting. ESP32 from Espresiff is used as a microcontroller, which is placed in the tank of the motorcycle and displays the measured data on the LCD display. The system also allows you to control the entire motorcycle, such as enabling starting or turning on the lights
|
|
|
Optimization of concentration determination of degrdation products of uracil via LC-MS
Kavale, Gustav ; Ševčík, Jan (advisor) ; Míšek, Jiří (referee)
In the Czech Republic, tens of thousands of oncology patients undergo treatment based on fluoropyrimidines every year. Many of these patients suffer from negative side effects that can even have a fatal impact. Currently, the best described factor in the cause of the toxicity of fluoropyrimidines is the activity of the pyrimidine catabolism enzyme - dihydropyrimidine dehydrogenase. Due to the high frequency of pathogenic mutations in the dihydropyrimidine dehydrogenase gene, the extreme risk of toxicity affects hundreds of patients in the Czech Republic every year. For this reason, the "European Medical Agency" has issued a recommendation to carry out investigations for the possible occurrence of toxic effects before starting treatment. One of the diagnostic options for determining sensitivity to fluorouracil is metabolomic analysis, based on the quantification of degradation products of uracil metabolism in plasma using liquid chromatography with mass spectrometry. The goal of this work is to perform basic optimization of mass spectrometer parameters for quantification of selected degradation products of uracil metabolism. Keywords: Uracil, Fluoruracil, Liquid Chromatography, Mass Spectrometry
|
|
| |
|
|
Study of population specific alterations of breast cancer predisposition genes in Czech Republic.
Judasová, Kristýna ; Ševčík, Jan (advisor) ; Holá, Dana (referee)
Breast cancer is the most frequent malignant disease in the female population worldvide. About 10 % of all cases are of hereditary origin. The inactivation of tumor suppressor gene BRCA1 is the main genetic predisposing factor in breast cancer in the Czech Republic. Primarily, BRCA1 participates in DNA double strand break repair. Depending on cell cycle phase, the damage is repaired by homologous recombination or non-homologous end joining. Alternative splicing variants of BRCA1 are frequently detected during the genetic screening of high risk patients. The clinical significance of these variants is unknown. Understanding of the nature of breast cancer genetics is the critical factor for early diagnosis. Based on earlier studies from the Institute of Biochemistry and Experimental Oncology 1st Faculty of Medicine Charles University, two alternative splicing variants which were repeatedly detected in patients, were chosen for functional analysis. The aim of this work is to investigate the impact of alternative splicing variants BRCA1Δ5 and BRCA1Δ10 on DNA double strand breaks repair. Particular variants were over- expressed in the cells of model system. Activity of homologous recombination (HR) and non-homologous end joining (NHEJ) was scored by in vitro DNA repair assay. The cellular localization of...
|
|
|
Diversity, systematics, and phylogeny of families Opetiidae and Platypezidae (Diptera)
Tkoč, Michal ; Prokop, Jakub (advisor) ; Ševčík, Jan (referee) ; Zatwarnicki, Tadeusz (referee)
5 ABSTRACT This doctoral thesis is focused on flat-footed fly families Opetiidae, Platypezidae and genus Microsania (Insecta: Diptera). The thesis consists of general introduction to the systematics, taxonomy, diversity, zoogeography, phylogeny, and biology of the flat-footed flies. Systematics and taxonomy are summarized based on historical as well as recent literature. Each important taxon is shortly introduced and information about its taxonomy, morphology, diversity, distribution, and biology is provided. The next two chapters deal with fossil species and research on species diversity. The following part is a summary and plan for future research. Final chapter consists of 9 published peer-reviewed articles (5 in impacted international journals and 4 in journals without impact factor). The first article is a molecular phylogeny study of relationships between genera of flat- footed flies. The results show Platypezidae consisting of two well-supported clades, the first with the subfamilies Melanderomyiinae + Callomyiinae and the second formed by subfamily Platypezinae. Genus Microsania was resolved as a separate lineage distant from Platypezidae which clustered with Opetiidae as its sister group, both together forming a sister group to Platypezidae. The genus Agathomyia proved to be paraphyletic....
|
|
|
Preprocessing of sewage sludge for pyrolysis process
Ševčík, Jan ; Rusník,, Igor (referee) ; Bodík,, Igor (referee) ; Hlavínek, Petr (advisor)
The concept of sludge management at the level of sludge treating technology of sewage treatment plants, as well as at national, continental or world level, requires fundamental improvement in the interest of sustainable development. Sewage sludge production is continuously increasing. Sewage sludge contains a number of pollutants but also a significant renewable resource for material transformation and / or energy recovery. Sewage sludge is composed of an inorganic mineral part and organic components. Its organic part is a source of energy and nutrients, but also contains harmful pollutants for exapmple heavy metals. However, there are technologies that offer the possibility of neutralizing pollutants while using other sludge components in terms of recycling energy, nutrients and other attractive sludge components. The mentioned technologies include mainly thermal methods of sewage sludge treatment and one of them is pyrolysis. Thessis focuses on sewage sludge prepartation for pyrolysis process, which includes mixing with additives and peletization of prepared mixture for purposes of agricultural or energetical utilisation. Technology for material transformation and energy utilization of sewage sludge, in particular the mentioned pyrolysis or torrefaction, require an insignificant degree of detailed research in order to expand it in the field of sludge management. Indeed, it is not only research that will enable direct correct use of this technology in practice. The results of such research are the basis for the drafting of the relevant legislation, which will subsequently enable the deployment of pyrolysis technologies in practice.
|
|
|
The functional in vitro analysis of the BRCA1alternative splicing variants
Ševčík, Jan ; Kleibl, Zdeněk (advisor) ; Stopka, Tomáš (referee) ; Macůrek, Libor (referee)
BACKGROUND: The inactivation of the tumor suppressor gene BRCA1 is a predisposing factor for a breast/ovarian cancer development. Formation of cancer-specific alternative splicing variants with aberrant biological properties can represent additional mechanism decreasing the overall BRCA1 activity in DNA double strand break (DDSB) repair. In this study, we analyzed BRCA1 alternative splicing variants BRCA114-15 and 17-19 ascertained previously during the screening of high-risk breast cancer individuals. METHODS: We established a stable MCF-7 cell line-based model system for an in vitro analysis of BRCA1 variants. Using this system, we analyzed the impact of BRCA114-15 and 17-19 variants on DNA repair kinetics using comet assay and confocal immunomicroscopy. The capacity of DNA repair was assessed directly by an in vitro NHEJ assay and indirectly by a mitomycin C sensitivity test. The proliferation activities were determined by a clonogenic assay and growth curves. RESULTS: Overexpression of BRCA114-15 and 17-19 increases the endogenous level of DNA damage, slows down the DDSB repair, and decelerates the initial phase of radiation-induced foci formation and prolongs their persistence. Moreover, BRCA114-15 and 17-19 differentially influence the activity of HR and NHEJ and sensitivity of MCF-7 cells to ionizing...
|
|
|
Design and manufacture of bending fixture
Ševčík, Jan ; Trčka, Tomáš (referee) ; Zemčík, Oskar (advisor)
The work deals with the design and manufacture of bending fixture. It contains a comparsion with current bending fixtures on the market and a justification for the production of a given type. Then the work contains a proposal of the constuction of individual parts with the selection and description of technologies of their production and the process of their production. The work also contains complete drawing ducumentation of all parts of the bending fixture. The conclusion of the work contains the possibilities of design modifications and economic and technological evaluation.
|
|
|
Analýza stavu ekologického zemědělství a bioprodukce ve vybraném regionu Zlínského kraje - okres KROMĚŘÍŽ
Ševčík, Jan
The diploma thesis „Analysis of the conditions of the ecological agriculture and bioproduction in the selected region Zlín, Kroměříž district deals with the contemporary conditions of the ecological agriculture and bioproduction in the Kroměříž district and with the availability of a range of goods for consumers. Principals and goals of the ecological agriculture, legislation, control mechanisms and production of the organic food are described in the literature review. History and development of the eco-agriculture in both the Czech Republic and the world are also described here. Next section focuses on the description of the Kroměříž district and its environmental conditions for agriculture as well as of the contemporary conditions of the eco-agriculture and production of the organic food. Last part focuses on the analysis of the companies in which the overall benefits for the region is found out through a questionnaire survey. The second questionnaire will be targeting directly on consumers of the organic food. The research findings and subsequent discussion are summarised in the conclusion.
|
|
| |
guest ::
RSS feed.