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Kinetics of selected substances in dermal exposure (Polycyclic aromatic hydrocarbons)
Kotingová, Lenka ; Fiala, Zdeněk (advisor) ; Bencko, Vladimír (referee) ; Čegan, Alexander (referee)
Kinetics of selected substances in dermal exposure (polycyclic aromatic hydrocarbons) Skin is one of the largest human body organs and its proper functioning is vital for human body. Dermal exposure represents one of the most important pathways of exogenous substances into the body. Data on kinetics of transdermal absorption of polycyclic aromatic hydrocarbons (PAH) have been yet so far insufficient. Present estimates of health risks level connected to dermal exposure to PAHs, are loaded with great deal of uncertainty. The aim of the dissertation thesis was employment and validation of appropriate testing method of transdermal absorption of substances in vitro and the use of this method to obtain new findings on the influence of concentration, dissolvent and form of application, on the measure of dermal absorption of selected PAH representatives. For the testing of transdermal absorption of substances in vitro, the method using static Franz vertical diffusion cell was chosen. As an absorption membrane, full leather of rear surface of the auricle of a domestic pig was used. Content of analytes in samples of receptor fluid was analyzed by gas chromatography with mass spectrometry (GC-MS) and by high performance liquid chromatography with fluorescence detection (HPLC), for which a new methods was developed and...
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Dermal Exposure of Polycyclic Aromatic Hydrocarbons (Selected Risks of Goeckerman Therapy of Psoriasis)
Málková, Andrea ; Borská, Lenka (advisor) ; Müllerová, Dana (referee) ; Čegan, Alexander (referee)
UNIVERZITA KARLOVA Lékařská fakulta v Hradci Králové Dermální expozice polycyklickým aromatickým uhlovodíkům (Vybraná rizika Goeckermanovy terapie psoriázy) Andrea Málková Autoreferát disertační práce Doktorský studijní program: Hygiena, preventivní lékařství Hradec Králové 2019 1 Summary Dermal exposure of polycyclic aromatic hydrocarbons (Selected risks of Goeckerman therapy of psoriasis) Polycyclic aromatic hydrocarbons represent a health-related group of substances commonly found in the living and working environment. Dermal form of exposure can make a very significant contribution to the overall burden on the body, and some authors estimate its proportion in severe exposures to almost half of the total absorbed dose of PAH. Contrary to this, dermal PAH exposure has so far received relatively little attention. For testing in vitro dermal penetration, the OECD recommends two models, full skin and epidermal membrane. In the presented text I present the results of an in vitro trans-epidermal penetration experiment. When penetrating selected PAHs through the epidermal membrane, we found higher Flux values and lower Lag time values compared to PAH penetration through the full skin described in the previous work. Penetration of PAHs through the epidermal membrane also showed a lower degree of data...
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Kinetics of selected substances in dermal exposure (Polycyclic aromatic hydrocarbons)
Kotingová, Lenka ; Fiala, Zdeněk (advisor) ; Bencko, Vladimír (referee) ; Čegan, Alexander (referee)
Kinetics of selected substances in dermal exposure (polycyclic aromatic hydrocarbons) Skin is one of the largest human body organs and its proper functioning is vital for human body. Dermal exposure represents one of the most important pathways of exogenous substances into the body. Data on kinetics of transdermal absorption of polycyclic aromatic hydrocarbons (PAH) have been yet so far insufficient. Present estimates of health risks level connected to dermal exposure to PAHs, are loaded with great deal of uncertainty. The aim of the dissertation thesis was employment and validation of appropriate testing method of transdermal absorption of substances in vitro and the use of this method to obtain new findings on the influence of concentration, dissolvent and form of application, on the measure of dermal absorption of selected PAH representatives. For the testing of transdermal absorption of substances in vitro, the method using static Franz vertical diffusion cell was chosen. As an absorption membrane, full leather of rear surface of the auricle of a domestic pig was used. Content of analytes in samples of receptor fluid was analyzed by gas chromatography with mass spectrometry (GC-MS) and by high performance liquid chromatography with fluorescence detection (HPLC), for which a new methods was developed and...
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