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In vitro screening of potential antimycobacterial compounds against fast growing strains of Mycobacterium genus
Židková, Lenka ; Janďourek, Ondřej (advisor) ; Jílek, Petr (referee)
Charles University Faculty of Pharmacy in Hradec Králové Department of Biological and Medical Sciences Study program: Specialist in Laboratory Methods Candidate: Bc. Lenka Lyčková Supervisor: PharmDr. Ondřej Janďourek, Ph.D. Title of diploma thesis: In vitro screening of potential antimycobacterial compounds against fast growing strains of Mycobacterium genus The aim of this work is to determine the efficacy of new compounds for possible treatment of tuberculosis in the future. A microdilution broth method with already proven procedure on Mycobacterium aurum and Mycobacterium smegmatis strains was used to determine the minimum inhibitory concentration of compounds. The theoretical part summarizes the epidemiological situation of recent years with global trends in the incidence and mortality of tuberculosis in the world and in the Czech Republic, including prospective plans for remediation in the time horizon. Mycobacteria are described in the main representation with M. tuberculosis. Tuberculosis disease - its history, forms, diagnosis and treatment with antituberculotics, which are divided according to the use lines, new drugs and drugs in development. The experimental part contains qualitative and quantitative methods of testing mycobacteria and captures the microdilution broth method used in...
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Kinetoplastida of birds and bloodsucking Diptera: molecular characterization and life cycles
Zídková, Lenka
We studied biodiversity of avian trypanosomes. We used newly obtained isolates of trypanosomatids from ornitophilic bloodsucking diptera (81) and from avian hosts (145). To investigate relationships among isolates we employed RAPD method and sequence analyses of SSU rRNA, eventually gGAPDH, 5S RNA or SL RNA. Additionally we used morphological characters; cell lengths and kinetoplast widths. All isolates obtained from biting midges belonged to monogenetic kinetoplastids. We described new species and a new genus Sergeia podlipaevi isolated from Culicoides (Oecacta) festivipennis a C. (O.) truncorum. We successfully infected with this species laboratory bred biting midge of different subgenus Culicoides (Monoculicoides) nubeculosus. We described isolate from Culicoides truncorum as new species Herpetomonas trimorpha. Its sister species was H. ztiplika described previously also from biting midges. We performed the most extensive study of biodiversity of avian trypanosomes based on comparison of wide spectrum of isolates obtained from different hosts. We confirmed that avian trypanosomes are polyphyletic and form three independent lineages within the genus Trypanosoma. We identified at least 11 species of avian trypanosomes. We found that ornitophilic mosquitoes belong among important vectors of avian...
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Kinetoplastida of birds and bloodsucking Diptera: molecular characterization and life cycles
Zídková, Lenka
We studied biodiversity of avian trypanosomes. We used newly obtained isolates of trypanosomatids from ornitophilic bloodsucking diptera (81) and from avian hosts (145). To investigate relationships among isolates we employed RAPD method and sequence analyses of SSU rRNA, eventually gGAPDH, 5S RNA or SL RNA. Additionally we used morphological characters; cell lengths and kinetoplast widths. All isolates obtained from biting midges belonged to monogenetic kinetoplastids. We described new species and a new genus Sergeia podlipaevi isolated from Culicoides (Oecacta) festivipennis a C. (O.) truncorum. We successfully infected with this species laboratory bred biting midge of different subgenus Culicoides (Monoculicoides) nubeculosus. We described isolate from Culicoides truncorum as new species Herpetomonas trimorpha. Its sister species was H. ztiplika described previously also from biting midges. We performed the most extensive study of biodiversity of avian trypanosomes based on comparison of wide spectrum of isolates obtained from different hosts. We confirmed that avian trypanosomes are polyphyletic and form three independent lineages within the genus Trypanosoma. We identified at least 11 species of avian trypanosomes. We found that ornitophilic mosquitoes belong among important vectors of avian...
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Phlebotomine saliva: the perspecives of application of some of their recombinantly produced proteins
Rožníčková, Lucie ; Zídková, Lenka (advisor) ; Štáfková, Jitka (referee)
Sandflies (Diptera: Phlebotominae) are the vectors of one of the most significant tropical diseases, leishmaniosis. Their saliva contains substances with antihemostatic, vasodilatory and immunomodulatory effect. These substances are of great significance in transmission of leishmaniosis, because of their ability to influence the host immune response. Naive host saliva modulate the immune system to such an extent, that it reduces an infective dose and increases the pathogenicity of leishmaniosis. On the other hand, a repeatedly exposed host generates a cellular and antibody immune response, which is able to make the initial development of infection impossible. Repeated exposure of individual sandflies lead to formation of antibodies against components of saliva, which may play a role as a rate exposure indicator and thus the risk of leashmaniosis infection. These proteins and glycoproteins can be produced by recombinant DNA technology. Recombinant vaccine could help in the fight against leishmaniasis. Keywords: saliva, sand fly, leishmania, recombinant proteins.
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Kinetoplastida of birds and bloodsucking Diptera: molecular characterization and life cycles
Zídková, Lenka ; Svobodová, Milena (advisor) ; Fiala, Ivan (referee) ; Modrý, David (referee)
We studied biodiversity of avian trypanosomes. We used newly obtained isolates of trypanosomatids from ornitophilic bloodsucking diptera (81) and from avian hosts (145). To investigate relationships among isolates we employed RAPD method and sequence analyses of SSU rRNA, eventually gGAPDH, 5S RNA or SL RNA. Additionally we used morphological characters; cell lengths and kinetoplast widths. All isolates obtained from biting midges belonged to monogenetic kinetoplastids. We described new species and a new genus Sergeia podlipaevi isolated from Culicoides (Oecacta) festivipennis a C. (O.) truncorum. We successfully infected with this species laboratory bred biting midge of different subgenus Culicoides (Monoculicoides) nubeculosus. We described isolate from Culicoides truncorum as new species Herpetomonas trimorpha. Its sister species was H. ztiplika described previously also from biting midges. We performed the most extensive study of biodiversity of avian trypanosomes based on comparison of wide spectrum of isolates obtained from different hosts. We confirmed that avian trypanosomes are polyphyletic and form three independent lineages within the genus Trypanosoma. We identified at least 11 species of avian trypanosomes. We found that ornitophilic mosquitoes belong among important vectors of avian...
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