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Crossover in Cartesian Genetic Programming
Vácha, Petr ; Vašíček, Zdeněk (referee) ; Sekanina, Lukáš (advisor)
Optimization of digital circuits still attracts much attention not only of researchers but mainly chip producers. One of new the methods for the optimization of digital circuits is cartesian genetic programming. This Master's thesis describes a new crossover operator and its implementation for cartesian genetic programming. Experimental evaluation was performed in the task of three-bit multiplier and five-bit parity circuit design.
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Point Counting on Elliptic and Hyperelliptic Curves
Vácha, Petr ; Šťovíček, Jan (advisor) ; Drápal, Aleš (referee)
In present work we study the algorithms for point counting on elliptic and hy- perelliptic curves. At the beginning we describe a few simple and ineffective al- gorithms. Then we introduce more complex and effective ways to determine the point count. These algorithms(especially the Schoof's algorithm) are important for the cryptography based on discrete logarithm in the group of points of an el- liptic or hyperelliptic curve. The point count is important to avoid the undesirable cases where the cryptosystem is easy to attack. 1
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Preparation of DNA binding domain of transcription factor Fox04 for tryptophan fluorescence measurements
Vácha, Petr
This thesis was worked up as a part of a project that is concerned with the manner of interaction of 14-3-3 proteins with forkhead transcriptional faktor FoxO4. 14-3-3 proteins are proteins interacting with a considerable amount of cell proteins. They affect activity and cell localization through this interaction. Transcriptional factor FoxO4 plays significant role in regulation of a cell cycle, apoptosis, respond to oxidative stress and many other cell proceses. Transcriptional aktivity of FoxO4 is regulated through phophorylation by protein kinases B. This phosphorylation induce inhibition of FoxO4/DNA binding, induce FoxO4/14-3-3 binding and thus relocalization of this complex from a cell core to cytoplasma. One of main goals of this project is to explain molecular mechanisms of these interactions. Main goal of this thesis was to create cDNA of a mutational protein FoxO4 containing just two tryptophan residues by site directed mutagenesis and after gaining this cDNA to express and pyrity this mutational FoxO4. This mutational protein was going to be a part of a model system for conformational changes studies. Besides expression and purification of 14-3-3 protein that is also part of our model system was performed.
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Study of interactions of forkhead transcription factor FOXO4 with DNA and the 14-3-3 protein
Vácha, Petr ; Obšil, Tomáš (advisor) ; Pavlíček, Jiří (referee) ; Krůšek, Jan (referee)
CHARLES UNIVERSITY IN PRAGUE THE FACULTY OF NATURAL SCIENCE Department of Physical and Macromolecular Chemistry The summary of the doctoral thesis Study of interactions of forkhead transcription factor FOXO4 with DNA and the 14-3-3 protein RNDr. Petr Vácha Scientific supervisor: Prof. RNDr. Tomáš Obšil, Ph.D. Prague 2015 Abstract This doctoral thesis deals with the interaction of human forkhead transcription factor FOXO4 with DNA and regulating 14-3-3 protein respectively. The main aim of this work was detailed characterization of interaction between DNA binding domain of protein FOXO4 with two canonical DNA sequences and further clarifying the role of the 14-3-3 protein in the regulation of activity of protein FOXO4. FOXO transcription factors are potent activators of the transcription of genes, which affect a variety of cellular processes. FOXO4 protein belongs to the family of forkhead transcription factor, which is a group of several tens of proteins, whose common feature is a highly conserved DNA- binding domain. Summary of the DNA binding specificity of these proteins, namely what precisely determines the small differences in the binding properties of individual forkhead proteins, despite the large amount of available structural data remains still unclear. Therefore, detailed characterization of...
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