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Historical Topographic Database of Luční bouda and Surroundings Based on Early Maps and Ortohphotos
Chytilová, Barbora ; Lysák, Jakub (advisor) ; Suchá, Renáta (referee)
HISTORICAL TOPOGRAPHIC DATABASE OF LUČNÍ BOUDA AND SURROUDINGS BASED ON EARLY MAPS AND ORTOHPHOTOS Abstract The bachelor thesis aims to creating the digital topographic database of the Luční Bouda region in Krkonoše capturing its development in time. The database contains early maps and orthophotos data taken within the area. Among crucial archival spatial data covered, in the database we can count maps of Stabilní katastr, III. military mapping, aerial snapshots from 1936, Map of the Czechoslovakia from 1937, Map of State in scale 1 : 5 000 as well as orthophotos taken in year 1953 and later on. The theoretical part of the thesis includes introducing the area of interest, hictorical cartography, actual data and theoretical information involving digital topographic database creation with time constituant. The research deals with project itself followed by procedure of filling the database with data. The conclusion involves brief discussion on the development of the area in time. Key words Luční bouda, Krkonoše, digital topographic database, early maps, orthophotos
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Changes of land cover above the tree line in Krkonoše National Park based on Landsat data
Vyvialová, Linda ; Červená, Lucie (advisor) ; Suchá, Renáta (referee)
CHANGES OF LAND COVER ABOVE THE TREE LINE IN KRKONOŠE NATIONAL PARK BASED ON LANDSAT DATA Abstract This study evaluates land cover changes in the area above the tree line in Krkonoše Mts. National Park. The stress is put on the changes of Pinus mugo shrub. Two methods of change detection based on Landsat data in four time horizons from the eighties up to now were tested (years 1984, 1992, 2002 and 2013). The first method was classification of scenes with the Maximum Likelihood Classifier individually and evaluation of changes based on their overlay. Overall accuracies from the oldest scene were 86,04 %, 88,44 %, 86,91 % and 86,43 %. The second method evaluates detection of change above one dataset that consists of scenes for all the time horizons. Overall accuracies were from the oldest 86.63 %, 88.64 % and 86.11 %. The second method was more appropriate for this study of land cover changes. On the map results there can be seen thinning of Pinus mugo scrub (between the years 2002 and 2013, 1984 and 1992) as well as its natural thickening and spreading. Key words KRNAP, tundra, land cover, change detection, Landsat
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Classification of selected vegetation land cover categories in the Krkonoše Mts. tundra from Sentinel-2A imagery using multi-temporal data
Roubalová, Markéta ; Kupková, Lucie (advisor) ; Suchá, Renáta (referee)
Classification of selected vegetation land cover categories in the Krkonoše Mts. Tundra from Sentinel-2A imagery using multitemporal data Abstract The aim of this thesis was to evaluate the possibilities of multi-temporal approach to improve classification accuracy of vegetation cover in eastern tundra in the Krkonoše Mts. National Park. Sentinel-2A imagery - 10 spectral bands with spatial resolution 10 and 20 m - was used. The classification legend was created by a botanist of the national park. Maximum likelihood classification for 11 categories of vegetation land cover was executed in software ENVI 5.3. The overall accuracy of the best classification result was 53,4 % which is similar result as in the case of single image classification (overall accuracy was 51,2 %). Key words: multi-temporal classification, vegetation, spectral features, Sentinel-2A, tundra, The Krkonoše Mts. National Park
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Historical Topographic Database of Luční bouda and Surroundings Based on Early Maps and Ortohphotos
Chytilová, Barbora ; Lysák, Jakub (advisor) ; Suchá, Renáta (referee)
HISTORICAL TOPOGRAPHIC DATABASE OF LUČNÍ BOUDA AND SURROUDINGS BASED ON EARLY MAPS AND ORTOHPHOTOS Abstract The bachelor thesis aims to creating the digital topographic database of the Luční Bouda region in Krkonoše capturing its development in time. The database contains early maps and orthophotos data taken within the area. Among crucial archival spatial data covered, in the database we can count maps of Stabilní katastr, III. military mapping, aerial snapshots from 1936, Map of the Czechoslovakia from 1937, Map of State in scale 1 : 5 000 as well as orthophotos taken in year 1953 and later on. The theoretical part of the thesis includes introducing the area of interest, hictorical cartography, actual data and theoretical information involving digital topographic database creation with time constituant. The research deals with project itself followed by procedure of filling the database with data. The conclusion involves brief discussion on the development of the area in time. Key words Luční bouda, Krkonoše, digital topographic database, early maps, orthophotos
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Changes in distribution of B lymphocyte subpopulations in patients with Crohn disease before and after biological therapy
Suchá, Renata ; Růžičková, Šárka (advisor) ; Krulová, Magdaléna (referee)
B-lymphocytes are lymphoid cells, which are a part of the adaptive/innate immune system and generate antibodies. Recently, many studies have supported hypothesis that different rather minor B-lymphocyte subpopulations may play a direct and indirect role in immunopathogenesis in human pathologies such as Crohn's disease (CD). The aim of current study was therefore to investigate distribution of frequencies of B lymphocyte subpopulations (from transient to mature effector B cell stages) in peripheral blood of healthy subjects (CO), patients with Crohn's disease (CD) and ulcerative colitis (UC). Thus, using 11-colour flow cytometry we have analysed 30 blood samples of individuals, including 14 healthy controls, 11 patients with Crohn's disease and 5 with UC. In 6 patients with CD we have had an opportunity to analyze blood samples collected 2 hours after an administration of anti-TNF therapy. Higher frequencies of memory B-lymphocytes (CD19+ CD27+ , CD19+ CD20+ CD27+ and CD19+ CD20+ CD27+ IgM+) were found in patients with CD as compared to COs. (20.06±13.58%; 17.61±13.48%; 88.60±20.56% vs. 11.75±26.47%; 11.25±26.50%; and 66.82±22.60%), in case of CD19+CD20-CD27-IgM+ B-lymphocytes the difference was statistically significant (57.15±17.21% in CD vs. 19.59±31.79% in CO; p=0.0341), which is in accordance...
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Assessment of the accuracy of Soil Sealing database in the Czech Republic
Hlaváčová, Iva ; Štych, Přemysl (advisor) ; Suchá, Renáta (referee)
Thesis is about European map of built-up areas with high resolution and the term about imperviousness (Soil Sealing Layer). The main aim of this bachelor thesis is to evaluate thematic accuracy of the Soil Sealing database in the Czech Republic with use of various statistical methods. The theoretical part deals with problems of impervious surfaces, outlines the framework of database development with principles of its creation. It is also about accuracy assessment of spacial data with specific examples. Validation is based on comparison of assessed grid with visually interpreted orthophotos. The comparison will take place within the targeted selection of three locations. Statistical methods will be applied on obtained data for particular areas and clarify accuracy of database depending on compactness of settlements. According to the results of validation, single methods and outcomes will be critically evaluated. Achieved results will be compared with similarly oriented studies. Powered by TCPDF (www.tcpdf.org)
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Assessment of lignin content in needles of Norway Spruce (Picea abies L. Karst.) using laboratory and image spectroscopy
Suchá, Renáta ; Kupková, Lucie (advisor) ; Brodský, Lukáš (referee)
The master thesis deals with determination of selected biochemicals (lignin, carotenoids, water) content in Norway spruce needles using laboratory and imaging spectroscopy. The first part of thesis summarizes literature dealing with methods of estimating lignin and other biochemicals content. Three types of data are used in this thesis: 1. spectra measured by contact probe and ASD FieldSpec 4 Wide Res spectroradiometer, 2. spectra measured by integrating sphere and spectroradiometer and 3. aerial hyperspectral image data acquired by APEX sensor. The most useful transformation methods - first derivative and continuum removal are applied to the spectrum. Further the linear relationship between measured spectrum and content of biochemicals is analysed. Stepwise multiple linear regression is applied to select suitable wavelengths for modeling of biochemicals content in spruce needles. The model is also calculated and applied on the level of image hyperspectral data. Maps of lignin content in Norway spruce are the final output of these part of this. Next part of the thesis compares spectra measured by contact probe and spectra measured by integrating sphere. Diffrerence between the studied areas based on biochemicals content in spruce needles and several chemical elements in the soil and based on...
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Mapping of sea ice extent by the help of remote sensing methods
Suchá, Renáta ; Potůčková, Markéta (advisor) ; Štefanová, Eva (referee)
Mapping of sea ice extent by help of remote sensing methods Abstract The aim of this thesis is to show utilization of remote sensing methods for monitoring of sea ice by way of an example of Beaufort Sea. In first part of this thesis resulting from the available information in the literature. A short principle of remote sensing is introduced. Some of observation satelites are mentioned, above all Envisat ASAR, whose data are used in the second part of this thesis. Further, some researches concerning the monitoring of sea ice are analysed. The applied visual and radar data are described in detail for monitoring of polar areas. The advantages and limit sof these data are mentioned. In the conclusion of this first part, the researches monitoring the sea ice in Beaufort sea are mentioned. The second part of this thesis is demonstrativ the extend of sea ice in two chosen areas in Beaufort sea. On the basis of eleven radar Picture provided for free from an European space agency, a classification of two type sof sea ice (first-year sea ice, ice floes/multiyear sea ice) was effected. In the watched areas, there was noted a larger extent of sea ice in 2003 than in 2009. The minimum of the ice area in both years was in the month of September. In the Picture, there is a perceptible progres of ice floes which is...
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The stability of biologically active compounds in elderberry (Sambucus nigra L.) inflorescence during processing
SUCHÁ, Renata
This thesis examines the stability of rutin in the inflorescences of the elder during processing. Elder (Sambucus nigra L.) is a medicinal plant that was used for medical purposes as early as in the Middle Ages. Both flowers (Flos Sambuci) and fruit (Fructus Sambuci) of the elder are on the list of official medicinal products of the State Institute for Drug Control. The main active phenolic substance is flavonoid rutin. Flavonoids are phenolic substances of plant origin. Among the best known properties of these substances is their antioxidant activity. They protect the low density blood lipoproteins from the oxidation modifications that cause the development of arteriosclerosis and hypertension. As a medical plant, the elder is used especially for the preparation of a medicinal drink. It is also commonly used as a material for the preparation of syrup. This product serves as a basis for a refreshing and tasty drink and lately is has also started to be sold in shops. For comparison, five single-flower syrups from the elderflower by different producers were bought. A method of preparation of the elderflower syrup was devised, based in one case on frozen elder inflorescences and in the other on dried inflorescences. The plant material came from wildly growing shrubs and it was collected in June 2013. Altogether, 8 syrups were prepared in the laboratory, 4 of them from fresh material and 4 from dried material. Two different methods were used. The first one was based on extraction using sugar solution, the second one on extraction using water solution where sugar was added afterwards. Another added substance was ethanol. The method for measuring the amount of rutin in the samples was a combination of two extraction steps. The first step was the usual exraction using aqueous methanol and the second step was extraction using a solid sorbent (SPE). The analysis of fresh and dried material concluded that dried elderflowers contain many times more rutin than fresh elder. Among the commercially produced syrups, the "Kitl Syrob Bezový" contained most rutin; a little less rutin was found in "Hamé Bylinný sirup Bezový květ". The concentration of rutin in the syrups prepared in the laboratory was much higher than in the commercial products. By using different methods of syrup preparation and their subsequent analysis, it was found that the syrups prepared from the dried inflorescences contained a higher amount of rutin than the syrups produced in the same way, but from frozen material. Addition of a small amount of ethanol had no effect on extraction of rutin from inflorescences. Free quercetin, which indicate a decomposition of rutin, wasn´t found in any of produced syrups. The produced syrups were used for the preparation of a drink and then underwent sensory analysis. The paired two-sided preference test included pairs of drinks consisting of a fresh elder drink and dried elder drink. The results showed that the evaluators preferred syrups from dried elderflowers in two of the cases, while in the other two evaluated pairs, the results were not statistically significant. In all cases, the evaluators most often found small and middle differences. The gained results show that the best method of syrup preparation uses dried plant material and sucrose solution as the extraction agent. The syrup prepared in this way contained most rutin and at the same time showed the smallest losses of this substance, which is beneficial to human health. Furthermore, it also tasted better in comparison with syrup prepared in the same way, but from fresh material.
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