National Repository of Grey Literature 421 records found  1 - 10nextend  jump to record: Search took 0.00 seconds. 
Analysis of intermediate filament structure by chemical cross-linking
Dlabolová, Lada ; Novák, Petr (advisor) ; Šulc, Miroslav (referee)
Intermediate filament proteins create a dynamic cytoskeletal filamentous network, which due to its elastic properties, significantly contributes to the resistance of cells and tissues to mechanical stress. An important protein from the family of intermediate filaments, vimentin, is expressed mainly in cells of mesenchymal origin. Vimentin has been associated with a large number of pathophysiological conditions, and current studies consider vimentin as clinically promising target for the diagnosis, prognosis and treatment of a wide range of diseases from cancer to infectious and inflammatory diseases. Although in terms of structural characterization, vimentin belongs to one of the most studied proteins from the family of intermediate filaments, our knowledge is currently limited to the structure of the vimentin tetramer. Vimentin is capable of self-assembly into filaments formed by homo-oligomeric ULF subunits and the assembly process involves several steps of the organization of subunits. Structural characterization of the oligomeric subunits involved in the assembly of vimentin filaments is a prerequisite for elucidating the architecture of mature filaments, which can significantly contribute to understanding and connecting the mechanisms of many diseases associated with changes in vimentin...
Studies on structure and biological functions of NKR-P1 receptors
Rozbeský, Daniel ; Novák, Petr (advisor) ; Konvalinka, Jan (referee) ; Drbal, Karel (referee)
Natural killer (NK) cells play a significant role in the detection and destruction of virally infected and tumor cells. The NKR-P1 receptors regulate NK cell function by an alternative missing-self recognition system. Although the NKR-P1 receptors were among the first surface NK receptors identified on rodent NK cells more than 20 years ago, there is still very little known about their biological function and their physiological ligands. Furthermore, no three-dimensional structure of any of the NKR-P1 family receptors has been published so far. To understand the functional architecture of mouse NKR-P1 receptors, we developed a simple and efficient protocol providing large amounts of pure soluble NKR-P1 proteins. The crystal structure of mouse NKR-P1A, determined at 1.7 A resolution, is the first structure of a representative of the NKR-P1 family. Crystal structure is formed by a compact C-type lectin-like domain and an extended loop that participates in domain swapping. A potential role of the swapped loop has been suggested in natural ligand binding by in silico studies. However, chemical cross-linking and H/D exchange in combination with high resolution mass spectrometry revealed this loop in close proximity to the compact core in solution. The discrepancy between the crystal and solution structure...
Interpretation of the drawing of an enchanted family in the context of social work with foster families
Gazdová, Marie ; Novák, Petr (advisor) ; Drahoňovský, Jan (referee)
The bachelor's thesis will be focused on the drawing of the "enchanted family". In the theoretical part, I deal with concepts related to the family. I also deal with surrogate family care, its forms, the perception of family care, etc. The theoretical text also contains an approach to the topic of children's drawing, especially its developmental stages, family drawings and drawings of the "enchanted family." The practical part is focused on drawing an "enchanted family" with children from foster families and talking to them. With the drawing of the enchanted family, it is essential that the child can express his view of the individual members of the family by bewitching them into an animal. Drawing in my research part is used to get to know the child's personality, especially his relationships between family members and his overall experience while drawing. I also decided on child development and substitute family care. Keywords Family, Foster Care, Child, Child Drawing, Family Drawing, Enchanted Family Drawing
Motivation and psychosocial needs of workers of the social and legal protection of children
Marková, Lucie ; Novák, Petr (advisor) ; Nová, Monika (referee)
This diploma thesis deals with social workers who work in the department of social and legal protection of children. The aim of the work was to characterize the motivation factors and psychosocial needs for the performance of the work of OSPOD social workers in Strakonice. My diploma thesis was divided into a theoretical part and a practical part. In the theoretical part of my diploma thesis, I described the basic concepts related to the concept of social-legal protection of children, including historical development, legislative framework, and I described the functioning system of social-legal protection of children in the Czech Republic. Furthermore, in this section I described terms such as the Code of Ethics for social workers, motivation, psychosocial needs, psychohygiene and burnout syndrome. In the practical part, the research strategy of qualitative research was used and the method of questioning and the technique of data collection in the form of a structured interview were chosen. Communication partners were employees of the Municipal Office in Strakonice, who work as social workers in the Department of Social and Legal Protection of Children. For the research in the practical part, the goal of the thesis, the main research question and three sub- research questions, which lead to the...
Sources of social support for immigrants from Eastern European states residing in the Czech Republic
Vaishle, Ihor ; Novák, Petr (advisor) ; Drahoňovský, Jan (referee)
The work focuses on the research of sources of social support among immigrants from Eastern European states (Russia, Ukraine, Belarus) legally residing in the Czech Republic. The thesis is divided into two sections. The theoretical part contains the conceptualization and description of basic terms. Empirical contains description of the methodology as well as analysis of the obtained data. The aim of this research was to find out what are the sources of social support for immigrants from the East Slavic ethnolinguistic area. Qualitative research methodology was used to conduct the research. For data collection was chosen a semi-structured interview, which was in the immigrants' native language. The study involved 12 respondents equally divided by nationality and sex. The research showed that the emotional and informational component of social support was the most important for immigrants. The main sources of social support for immigrants include the immediate environment: partner, friends and family. Keywords: social support, immigration, migrants from Eastern European states, integration, social work, intercultural communication.
Social worker as an employee and member of a (multidisciplinary) team
Kolářová, Barbora ; Novák, Petr (advisor) ; Drahoňovský, Jan (referee)
This diploma thesis deals with a social worker as an employee and a member of a multidisciplinary team in the field of prison system. The aim of the thesis was to find out the view on the social worker from the perspective of top management of the Czech prison service. The theoretical part is divided into three major chapters that interconnect with each other. The first chapter deals with the basic definition of social work and a multidisciplinary team, but also delves into the specific area of social work with the crime perpetrators. The second chapter deals with general management, management of social work and top management, which plays a major role in the research investigation. The third chapter focuses on the area of prison system, its brief history, structure, and for the purposes of adding some interesting information to the thesis, also touches upon the life behind prison bars. The empirical part used qualitative research, specifically semi-structured interview, to fulfill the research goal. The interviews were conducted with top management of the Czech prison service. All collected data was analyzed using method, inspired by grounded theory approach. During the preparation of this thesis, I found out that the view of top management on social workers is completely positive. According to...
Critical analysis of attitudes of the professional public to Montessori approach in social work with children
Richterová, Daniela ; Novák, Petr (advisor) ; Nová, Monika (referee)
The aim of this diploma thesis is to find out the attitudes of social workers who work with children as a target group towards the Montessori approach. This thesis deals with what social workers think about this specific approach, what they think about it and where they see the space for its use in their professional practice. In the theoretical basis of the work, 3 basic topics are presented: social work, Montessori approach and attitudes. The part on social work deals with defining social work and the profession of a social worker, including the legislative framework, it also deals with the specification of social work with children and the introduction of institutions where it is possible to work with children within this profession (e.g. Authority for the social and legal protection of children, low-threshold facilities for children and youth, children's crisis centres, etc.). The section on Montessori is devoted to this method and the basic pillars on which it stands. A sub-chapter is also dedicated to the founder Maria Montessori and the origin of the method itself. The last theoretical area describes attitudes, their classification and answers the questions of how attitudes are formed, what they are good for, what leads to their change and how they can be measured. Research is processed in...

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