National Repository of Grey Literature 53 records found  1 - 10nextend  jump to record: Search took 0.01 seconds. 
Noncanonical functions of IL-1α
Novák, Josef ; Pospíšek, Martin (advisor) ; Černý, Jan (referee) ; Brdička, Tomáš (referee)
1α (IL 1α) is a multifunctional cytokine 1α is 1α independent on the receptor sig 1α is responsible for 1α to the plasma membrane. 1α activates express κB, binds to 1α 1α 1α to the plasma membrane 1α to signal 1α is required for membrane 1α exter 1α anchoring 1α 1α 1α with tumor suppressor p53 following genotoxic stress is further described in human cell 1α coloca
Molecular mechanisms in the development of the cochlea
Procházková, Šárka ; Pavlínková, Gabriela (advisor) ; Novák, Josef (referee)
Molecular mechanisms in the development of the cochlea The cochlea is the primary structure for the perception of sound. A spiraled cochlear duct contains the auditory organ, the organ of Corti, is innervated by sensory neurons of the spiral ganglion. The organ of Corti contains hair cells, the sensory receptors of the inner ear, that are responsible for the mechano-transduction of sound waves into electrical signals. The development of the cochlea is regulated by the cellular and molecular processes, including cochlear outgrowth, cellular differentiation, and patterning. The precise coordination of multiple processes during the transformation of a flat embryonic epithelium into the cellular mosaic of the adult structure requires a multitude of transcription factors and signaling pathways. These regulatory networks dictate cell fate and timing decisions during cochlear development. Interestingly, these mechanisms operate as well during sensory epithelial cell regeneration after damage and during stem cell directed differentiation, making developmental studies instrumental for improving therapies for hearing impairment.
Structure, molecular functions and oncogenic potential of suprabasin
Krátký, Marek ; Novák, Josef (advisor) ; Pimková, Kristýna (referee)
Suprabasin is a relatively recently discovered protein, originally studied for its role in the formation of the cornified envelope and keratinocyte differentiation. In recent years, research has focused on the oncogenic potential of suprabasin and its role in various types of diseases (e.g. myelodysplastic syndrome, atopic dermatitis or psoriasis). One of the isoforms of suprabasin has a predicted unique β-solenoid domain in its structure, which is characterized for the first time in this work. In addition to general information about the gene and isoforms of the suprabasin protein, the paper also summarizes important findings about the interaction of suprabasin with proteins of the EGFR/STAT3/Src, Wnt/β-catenin, NF-κB and AKT signaling pathways. The paper relates these findings to tumor properties to which suprabasin contributes, such as the ability of tumors to metastasize, immunosuppression and angiogenesis. The summary of the findings in this paper raises questions for future research, which undoubtedly holds important information about the structure, function and oncogenic potential of suprabasin. Keyword: Suprabasin, unconventional secretion, oncogenic potential, biomarker, cornified envelope
3D models of brain tumors
Fišer, Ondřej ; Novák, Josef (advisor) ; Bohačiaková, Dáša (referee)
Despite intensive research, glioblastoma multiforme remains one of the tumours of the central nervous system with the worst prognosis. The ability of glioblastoma cells to infiltrate brain tissue by forming invasive microtubular structures is stimulated by contact with adjacent non- tumor cells. Intercellular communication and the influence of the extracellular matrix create a specific microenvironment that affects cell signaling, proliferation, differentiation and response to pharmaceuticals. The recurrent form of glioblastoma often displays a much faster progression than the initial disease, which is attributed to the development of resistance to therapeutics and the preservation of the proliferative capacity of some tumour cells. The discovery of the stem- cells ability to self-aggregate in suspension has led to the creation of 3D in vitro models - brain organoids. They are much more complex that the established 2D models and their heterogeneity provides an environment simulating the in vivo state. This thesis aims to describe their use in brain tumour research and techniques for culturing 3D aggregates of neural lineage formed from induced or embryonic human stem cells with respect to their gradually increasing complexity. It also presents methods of addressing issues of hypoxia, organoid...
Musical aspects of the Living History phenomenon in the Czech historical fencing through the perspective of ethnomusicology
Novák, Josef ; Jurková, Zuzana (advisor) ; Seidlová, Veronika (referee)
The aim of this musical anthropology thesis focuses on "medieval music" as practiced by fans of historical fencing in the form of living history on the example of two Czech bands - Subulcus and Medieval Open Band as part of project Prácheňská manství. In my thesis I focus not only on sound and musical instruments, but also on the behavior of actors and their conceptualization of the Middle Ages, according to the concepts of music as culture of Allan Merriam, resp. of music as social life of Thomas Turino. I combine here the basic ideas of ethnomusicology with the ideas of musical memory in connection with the contemporary concepts of social memory and, last but not least, with the concepts of "golden age", nostalgia, staged authenticity and invented traditions. The point of this work is to bring about the origin and form of music that the actors understand as medieval, although not always comes the repertoire from the Middle Ages.
Study of the mammalian oncogenic transcription factors in the yeast model
Novák, Josef ; Zámostná, Blanka (advisor) ; Šťovíček, Vratislav (referee)
Yeast serves as a useful tool for studying cellular processes and therefore a large amount of techniques and protocols has been developed. There are special methods for studying the transcriptional factors in yeast, such as modified yeast two-hybrid screens, yeast one-hybrid screens and systems studying ability of transcription factors to transactivate a reporter gene. Oncogenes from AP-1 complex, Myc and Myb protein families are described in this work. Using a yeast model the structural-functional properties of proteins can be easily studied and in some cases even their ability of oncogenic transformation can be predicted (FASAY or ability of c-Myc to transactivate a reporter gene). However, results from yeast models must be confirmed in mammalian cells. 1
The Influence of the Economic Crisis on Corporate Social Responsibility Activities
Potůčková, Petra ; Rynda, Ivan (advisor) ; Novák, Josef (referee)
The aim of this thesis is to introduce the concept of Corporate Social Responsibility (CSR) and describe the relevance of corporate social responsibility activities. Due to the fact that, one of the basic characteristics of Corporate Social Responsibility, is long-term in character, which requires a dedication to CSR activities, even in times of financial difficulties when company does not have the optimal results, this thesis shows how selected companies fulfill the concept of CSR. The theoretical part is divided into two parts - the first is the concept of Corporate Social Responsibility, which focuses on the development, pillars, benefits and concepts associated with this concept. I deal with crisis and describe financial crisis, economic crisis, its development, causes and consequences in the next part. The empirical part of this thesis is based on the methodological triangulation - expert interviews, documented examination and comparison. This thesis sampled five selected companies and shows how these companies perceive CSR, what activities they have implemented, how to cope with the crisis that affected them and how it affected the implementation of CSR activities. Another benefit of this thesis is to expand knowledge about values of companies around the city of Kolín. The results of this...
Analysis of indicators of the social environment in the municipalities on the basis of various criteria for use at the local level
Galgonek, Dina ; Janoušková, Svatava (advisor) ; Novák, Josef (referee)
This diploma thesis deals with analysis of indicators of the social environment in small municipalities with up to 2 000 inhabitants based on various criteria for local use. The aim of the thesis is to propose an indicator set, which will best meet the requirements of such set in terms of applied criteria. The research question is: What indicators should be included in an indicator set for assessing the social environment of municipalities with up to 2 000 inhabitants to suit the differences and needs of these communities? The research problem addressed in the thesis concerns the possibility of measuring social sustainability at the local level, focusing on small municipalities with up to 2 000 inhabitants in the Czech Republic. The thesis also focuses on the possibility of projecting SDGs into this set. It is a qualitative research that involves the use of methods of document analysis, comparison, description, non-participant observation and semi-structured interviews. Based on the first four methods and theoretical knowledge, a preliminary design of the indicator set was created. Within the framework of semi-structured interviews, this design was discussed with representatives of selected small municipalities and other stakeholders. The edited set represents the final indicator set of the social...
Generation of conditional animal mutants to study gene function in vivo
Herrmannová, Pavlína ; Sedláček, Radislav (advisor) ; Novák, Josef (referee)
Conditional gene targeting allows spatial and temporal control of genetic modifications and is used to study gene functions in specific tissues or cell types. Gene targeting may lead to inactivation of the gene by insertions or deletions. Conditional gene targeting uses various methods for generation of transgenic mutant animals, such as technology of targeted disruption of gene using embryonic stem cells, methodology based on bacterial artificial chromosomes, or a new revolutionary technology of targeted disruption of genes using programmable nucleases, which is rapidly evolving and seems to be more efficient and cheaper method for conditional gene targeting. The aim of this work is to overview methods and technologies for generation conditional animal models, and overview conditional recombination systems with emphasis on inducible systems, and also provides a summary of the main international resources for rodent mutagenesis. Key words: transgenic animal model, gene, targeting, conditional allele

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