National Repository of Grey Literature 44 records found  1 - 10nextend  jump to record: Search took 0.00 seconds. 
The effect of temperature on the charging and discharging characteristics of lithium-ion batteries
Doleželová, Eva ; Máca, Josef (referee) ; Libich, Jiří (advisor)
This thesis deals with operation principles of lithium-ion batteries, its degradation principles, capacity fade, materials used, formats, main characteristics, development, uses and mid to high temperature behaviour. The thesis aims to study aging characteristics of commercially available lithium-ion batteries. In the practical part of the thesis, the key parameters of selected types of commercially available lithium-ion batteries were measured under normal temperature conditions. The measurement was repeated at an elevated temperature. Results were analysed and effects of different temperatures on the selected characteristics were concluded both verbally and graphically. A conclusion evaluates short-term and long-term impacts of ambient temperature on rate capability, ageing, capacity fade and longevity.
The studying of some markers of transforming growth factor pathway in mouse model of atherosclerosis.
Pagurko, Lukáš ; Nachtigal, Petr (advisor) ; Doleželová, Eva (referee)
Abstracts Lukáš Pagurko The studying of some markers of transforming growth factor pathway in mouse model of atherosclerosis. Diploma thesis Charles University in Prague, Faculty of Pharmacy in Hradec Králové Pharmacy Background: We observed the expression of endothelial nitric oxide synthase (eNOS) in mice atherosclerotic plaque by using imunohistochemical methods. The aim was to prove nonlipid-lowering effects of atorvastatin to affect the expression of eNOS. Methods: Female apoE and LDLr deficient mice, aged 16 weeks were used in the study. Biochemical analysis of blood samples and imunohistochemical analysis of the tissue segments were performed. For identification of eNOS expression was used EnVision method with DAB visualization. Results: We found out in biochemical analysis that 8-weeks doses of atorvastatin significantly increases levels of overall cholesterol. Presence of atherosclerotic lesions was observed at all incisions in control group even in atorvastatin group, whereas aterom necrotic fibres was founded at a number of them. The administration of atorvastatin were lead to significant decrease of atherosclerotic lesions compared to untreated group. There were also rapid increase of eNOS expression especially on the surface of atherosclerotic lesions. Conclusion: The positive influence of...
The study of VCAM-1 expression in experimental atherogenesis
Mertlíková, Veronika ; Nachtigal, Petr (advisor) ; Doleželová, Eva (referee)
Charles University in Prague Faculty of Pharmacy in Hradec Králové Department of Biological and Medical Sciences Candidate: Veronika Mertlíková Supervisor: Doc. PharmDr. Petr Nachtigal Ph.D. Title of diploma thesis: The study of VCAM-1 expression in experimental atherogenesis. The aim of the thesis is to describe the expression of VCAM-1 in the vessel wall of normocholesterolemic C57BL/6J mice, which were fed standard laboratory diet, and hypercholesterolemic apoE-deficient mice. As an experimental model normocholesterolemic C57BL/6J male mice and apoE-deficient mice at the age of 6 weeks were used. The C57BL/6J male mice were fed standard laboratory diet and apoE-deficient mice were fed cholesterol diet for 8 weeks. After this procedure a biochemical analysis of blood and immunohistochemical analysis of segments taken from aortic sinus and aortal arch were made. In comparison with the control C57BL/6J mice a large level of cholesterol was found in the blood of the apoE-deficient mice. Atherosclerotic lesions are thus generated only by the hypercholesterolemic mice. We were monitoring the expression of VCAM-1 in aortas of the normocholesterolemic C57BL/6J mice without atherosclerotic lesions by immunohistochemical methods, and the same methods were used for monitoring of the areas with and without...
Alteration of transport proteins expression during intrahepatic cholestasis in rats
Kolouch, Jan ; Doleželová, Eva (advisor) ; Cermanová, Jolana (referee)
Jan Kolouch Alteration of transport proteins expression during intrahepatic cholestasis in rats Diploma thesis Charles University in Prague, Faculty of Pharmacy in Hradec Králové Pharmacy Background: The aim of the diploma thesis was the verification of cholestatic liver injury by biochemical analysis of plasma and analysis of the main efflux and uptake hepatic transporters (Ntcp, Oatp1a4, Bsep, Mrp2, Mrp3, Mrp4) at the mRNA level. Methods: Wistar rats (n = 6, in each group; 280 - 320 g) were divided into two groups: Control group (LPS-K) and LPS group (lipopolysaccharide was administred at once i.p., 4mg/kg). Blood and liver samples were collected 12 hours after LPS administration. Biochemical analysis of plasma was performed by Cobas Integra ® 800 and GC/MS. Changes of mRNA expression of the transporters were evaluated by qRT-PCR. Results: In comparison to control group, LPS group showed significantly elevated plasmatic levels of the bile acids and also elevated activity of ALP and GMT. Plasmatic levels of bile acids were elevated to 818%, activity of ALP was elevated to 311% and activity of GMT to 2167%. Intrahepatic cholestasis led to the significant decrease of mRNA levels - Ntcp to 4.6%, Oatp1a4 to 2.2%, Mrp2 to 1.7% and Bsep to 25%. Conclusion: According to the results of this study, it is...
The study of transforming growth factor receptor during atherogenesis in mouse model of atherosclerosis.
Vybulková, Lucie ; Nachtigal, Petr (advisor) ; Doleželová, Eva (referee)
Lucie Vybulková The study of transforming growth factor receptor during atherogenesis in mouse model of atherosclerosis Diploma thesis Charles University in Prague, Fakulty of Pharmacy in Hradec Králové Pharmacy Background: The aim of this diploma thesis was the establishment of methodology for detection of TGF-βRI, subtype ALK-5, and studying its expression in the aortic root in apoE/LDLr- deficient mice. Methods: Using female mice C57BL/6J strain with a double deficit of apolipoprotein E and LDL-receptor was performed biochemical analysis of blood specimens, histologic staining with oil red for detection of lipids in tissues and immunohistochemical analysis of segments of tissue from the aorta and the top half of the heart. To detect expression of ALK-5 was used EnVision method with DAB visualization. Results: Using biochemical analysis, we confirmed increased levels of cholesterol in mice fed cholesterol diet as compared with mice on standard diet. At histologic staining with oil red we detected increased formation of atherosclerotic lesions in mice with cholesterol diet. Immunohistochemical analysis of expression of ALK-5 we demonstrated high expression of ALK-5 in the tunica media and in atherosclerotic plaques with no significant difference in the two groups of mice. Conclusion: The results...
Immunohistochemical Analysis of Expression of Phosphorylated form Smad2 after Administration of Atorvastatin in Experimental Atherogenesis
Vybulková, Lucie ; Nachtigal, Petr (advisor) ; Doleželová, Eva (referee)
Charles University in Prague, Faculty of Pharmacy in Hradec Králové Department of Biological and Medical Sciences Candidate Mgr.Lucie Vybulková Consultant Doc.PharmDr.Petr Nachtigal, PhD Title of Thesis Immunohistochemical analysis of expression of phosphorylated form Smad2 after administration of atorvastatin in experimental atherogenesis Background: The aim of this thesis was to describe the effects of atorvastatin treatment on the expression of phosphorylated form of Smad2 in atherosclerotic plaques in ApoE/LDLr-deficient mice by immunohistochemical methods. Furthermore, the influence of atorvastatin on lipid spectrum and size of atherosclerotic plaques was observed. Methods: In our experiment there were used C57BL/6J female mice with double deficit of apolipoprotein E and LDL receptor. There was performed the biochemical analysis of blood samples, histological staining with oil red for detection of tissue lipids, quantitative analysis of size of lesions and immunohistochemical analysis of samples containing semilunar valves and aorta for the detection of expression of Smad2. It was done using the method Avidin-Biotin and detection with DAB. Results: Using biochemical analysis we confirmed increased levels of total cholesterol and VLDL after the administration of atorvastatin. Smaller size of...
Enterobius vermicularis
Exnerová, Andrea ; Vejsová, Marcela (advisor) ; Doleželová, Eva (referee)
Andrea Exnerová Enterobius vermicularis Bachelor thesis Charles University in Prague, Faculty of Pharmacy in Hradec Králové Medical laboratory technician Background: The aim of this thesis is to create an overview of the current knowledge about Enterobius vermicularis and to perform a questionnaire study among parents. The aim of this survey is to find out informations about the progress, diagnosis and treatment of this infection and public awareness of this disease. Methods: Questionnaire study among parents, whose children have ever been infected with Enterobius vermicularis. Results: Pinworm infection is the most common among children aged 4-7 years. Half of the children who visited a pediatrician were examined. In almost 25 % of the cases, the stool sample was collected. The children were treated mostly with prescribed medication, the parents rarely chose home-treatment. In one third of the cases, the other members of the household were infected too. In the half of the cases the whole family was treated preventively. The most common symptom is rectal itching (83 %). Complications are exceptional. 60 % of the respondents incorrectly think that it is possible to get infected with pinworms from animals. The respondents most commonly called enterobiasis a disease of dirty hands. Conclusions: The...
Progression of endothelial dysfunction in heart in a mouse model of atherosclerosis.
Filipová, Petra ; Němečková, Ivana (advisor) ; Doleželová, Eva (referee)
Charles University in Prague, Faculty od Pharmacy in Hradec Králové Department of Biological and Medical Sciences Progression of endothelial dysfunction in heart in a mouse model of atherosclerosis Author of diploma thesis: Petra Filipová Supervisor: RNDr. Ivana Němečková, Ph.D. Backround: Atherosclerosis is a disease when atherosclerotic lesions are formed in vessels. It represents the major cause of the heart failure and stroke. The endothelial dysfunction, which has been recorded in a large number of animals, is considered one of the main causes of atherosclerosis. In our work we have focused on the detection of the endothelial dysfunction markers in the heart of the ApoE/LDLR double knock-out mouse model. Methods: We detected changes in the expression of eNOS, p-eNOS and HO-1 in two-months and eight-months old ApoE/LDLR double knock-out mice, which were fed standard chow diet. For the semiquantitative evaluation of the observed markers we used the Western blot analysis of the homogenated left ventricle. Results: According to our results there is no difference in the expression of eNOS, p-eNOS and HO-1 in the studied mouse model of atherosclerosis in relation to age. This might mean, that the endothelial dysfunction in the coronary vessels of these mice doesn't develop. However, for further...
Spirulina Platensis effects on atherogenesis in mouse model of atherosclerosis
Žáková, Marie ; Nachtigal, Petr (advisor) ; Doleželová, Eva (referee)
Marie Žáková Spirulina platensis effects on atherogenesis in mouse model of atherosclerosis Diploma thesis Charles University in Prague, Faculty of Pharmacy in Hradec Králové Pharmacy Background: The aim of this diploma thesis was to evaluate possible antiatherogenic and antiinflammatory effects of Spirulina platensis in apoE-deficient mice. Methods: We used C57BL/6J male mice with deficiency of apolipoprotein E (apoE-/-) at the age of 3 months. The animals were divided into 2 groups (control and examined mice). Both groups were fed with cholesterol diet for 8 weeks. In Spirulina group, Spirulina platensis was added at dose of 20 mg/kg/day. The biochemical analysis was performed in order to discover the lipid spectrum of blood. There was also performed the histological staining with oil red in order to visualise the size of atherosklerotic leisons and to detect the lipids. The aim of the immunohistochemical analysis was to approve the amount of VCAM-1 expression. Results: Biochemical analysis did not demonstrate any significant decrease of total cholesterol expect from decreasing of LDL cholesterol. The presence of atherosclerotic lesions with lipids cumulation was illustrated by histological staining with oil red in both mice groups, the results of each group did not differ. In comparison with...

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