|
|
Methodology for assessing the effect of sublethal doses of pesticides on solitary bees employing the OMICs approach
Erban, Tomáš ; Shcherbachenko, Elena ; Šlachta, Martin ; Cudlín, Pavel ; Chalupníková, Julie ; Halešová, Taťána ; Tomešová, Daniela ; Václavíková, Marta ; Votavová, Alena
Pollinators are potentially exposed to a number of pesticides in the environment. The most important group of hazardous substances comes from the category of plant protection products (PPPs), which are applied not only by farmers but also by small gardeners. Each PPP including the active substance and formulation undergoes a very detailed and thorough evaluation, which is in the European Union (EU) based on Regulation (EC) No 1107/2009 of the European Parliament and of the Council. Although the negative effects of PPPs on the environment and non-target organisms are minimized with proper use, we cannot rule out possible hidden effects, especially those of a sublethal nature. Modern analytical approaches, such as the application of high-throughput methods, help to identify hidden side effects. This methodology is focused on the evaluation of the effect of sublethal doses of pesticides on solitary bees employing the OMICs approach. In the risk assessment of pesticides, the honey bee has long been preferred as a model species of pollinator, while solitary bees stay in the background. The methodology contains innovative aspects for experiments, such as a modified isolator, preparation of plants for exposure, the timing of the experiment, monitoring of active substance distribution or proteomic analysis of solitary bees to identify hidden effects of the test substance or its formulation. In this methodology, the model of the solitary bee, red mason bee (Osmia bicornis), is considered. However, the methodology is also applicable to other, especially related species of solitary bees. The methodology can be used in areas of state administration, private laboratories and research activities in the assessment of environmental risks of pesticides on solitary bees. The methodological procedure can confirm or even eliminate environmental risks when registering new products or reevaluating existing products. This methodology therefore has the potential for use in testing new substances intended for plant protection before their registration. In an exemplary embodiment, the effect of acetamiprid in a Careo sticks formulation was tested. The distribution of acetamiprid in the soil substrate and the plant was evaluated. The hazardous metabolite acetamiprid IM-2-1 was identified in plant tissues. However, proteomic analysis indicated a very low risk of acetamiprid for O. bicornis. This result is in agreement with the fact that acetamiprid has been assessed by EFSA to be a low risk for bees and is registered in the EU until 28 February 2033.
Fulltext:  PDF
|
|
|
Assessment of ecosystem functions of urban greenery
Zemek, František ; Pikl, Miroslav ; Pavelka, Marian ; Zapletal, Miloš ; Kokrda, Lukas ; Novotný, Jan ; Duchková, Helena ; Vačkářová, Davina ; Foltýnová, Lenka ; Cudlín, Pavel ; Kolařík, J. ; Holešová, H. ; Mácová, Kateřina ; Chalupová, O. ; Vojáčková, B. ; Mikulášek, J.
The methodology brings about new and practical approaches in assessment of five ecosystem functions and services of town greenery. It covers four regulation functions (microclimate regulation, carbon sequestration, pollution and noise reduction) and one information function (aesthetic and cultural-recreation). The calculus is based on a single greenery object evaluation using simplified biophysical and statistic models, however complying with all natural principles. The models utilize data standardly available in urban greenery passports and those which are accessible via map servers, e.g. meteorological situation and sociodemographic conditions.
|
|
|
Measures to support populations of solitary bees in agroecosystems
Šlachta, Martin ; Erban, T. ; Votavová, A. ; Cudlín, Ondřej ; Cudlín, Pavel ; Halešová, T.
Solitary bees participate in the pollination of crops. According to our field\nsurvey, this involves 88 species of solitary bees in spring, mainly mining bees\n(Andrenidae) and sweat bees (Halictidae) nesting in the soil. The species\nnesting above soil were present less due to the lack of nesting opportunities\nand generally summer nesting activity. Among them, mason bees (Osmia sp.),\nnesting in spring, are used abroad for the pollination of fruit trees. The aim of\nthis methodology is to provide farmers with instructions for the use of mason\nbees for pollination of orchards by means of artificial nesting sites. We propose\na convenient construction of nesting sites and the methodology for rearing the\nEuropean orchard bee, Osmia cornuta (Latreille, 1805), which is, due to its earlyspring nesting activity, a suitable substitute for the currently used buff-tailed\nbumblebee. Breeding can be supplemented by the red mason bee, O. bicornis\n(Linnaeus, 1758), with later nesting activity. The book contains also suggested\nmeasures to support solitary bees in the landscape and new research data on\nthe diversity of solitary bees and pollen preferences of mason bees.
|
|
|
Estimate of carbon sequestration in the Czech Republic - afforestation of arable land, change of forest management, wetlands renaturations and tree lanes
Hruška, Jakub ; Pavelka, Marian ; Chuman, Tomáš ; Oulehle, Filip ; Vindušková, O. ; Cudlín, Pavel ; Trnka, Miroslav
Report acconunted quantitetivly as well qualitativly (for the year 2050) carbon sequestration for afforestation of arable land, change of forest management, wetlands renaturations and tree lanes.
|
|
|
Methodology for assessing the effect of sublethal doses of pesticides on solitary bees\nemploying the OMICs approach
Erban, T. ; Shcherbachenko, E. ; Šlachta, Martin ; Cudlín, Pavel ; Chalupníková, J. ; Halešová, T. ; Tomešová, D. ; Václavíková, M. ; Votavová, A.
Pollinators are potentially exposed to a number of pesticides in the environment. The\nmost important group of hazardous substances comes from the category of plant protection\nproducts (PPPs), which are applied not only by farmers but also by small gardeners. Each PPP\nincluding the active substance and formulation undergoes a very detailed and thorough evaluation,\nwhich is in the European Union (EU) based on Regulation (EC) No 1107/2009 of the European\nParliament and of the Council. Although the negative effects of PPPs on the environment and nontarget organisms are minimized with proper use, we cannot rule out possible hidden effects,\nespecially those of a sublethal nature. Modern analytical approaches, such as the application of\nhigh-throughput methods, help to identify hidden side effects. This methodology is focused on the\nevaluation of the effect of sublethal doses of pesticides on solitary bees employing the OMICs\napproach. In the risk assessment of pesticides, the honey bee has long been preferred as a model\nspecies of pollinator, while solitary bees stay in the background. The methodology contains\ninnovative aspects for experiments, such as a modified isolator, preparation of plants for exposure,\nthe timing of the experiment, monitoring of active substance distribution or proteomic analysis of\nsolitary bees to identify hidden effects of the test substance or its formulation. In this methodology,\nthe model of the solitary bee, red mason bee (Osmia bicornis), is considered. However, the\nmethodology is also applicable to other, especially related species of solitary bees. The\nmethodology can be used in areas of state administration, private laboratories and research\nactivities in the assessment of environmental risks of pesticides on solitary bees. The\nmethodological procedure can confirm or even eliminate environmental risks when registering new\nproducts or reevaluating existing products. This methodology therefore has the potential for use in\ntesting new substances intended for plant protection before their registration. In an exemplary\nembodiment, the effect of acetamiprid in a Careo sticks formulation was tested. The distribution of\nacetamiprid in the soil substrate and the plant was evaluated. The hazardous metabolite\nacetamiprid IM-2-1 was identified in plant tissues. However, proteomic analysis indicated a very\nlow risk of acetamiprid for O. bicornis. This result is in agreement with the fact that acetamiprid\nhas been assessed by EFSA to be a low risk for bees and is registered in the EU until 28 February\n2033.
|
|
|
Comparison of gradient measurement and modelling of deposition rate of ozone to spruce forest in locality Bílý Kříž.
Zapletal, M. ; Chroust, P. ; Večeřa, Zbyněk ; Mikuška, Pavel ; Cudlín, Pavel ; Urban, Otmar ; Pokorný, Radek ; Czerný, Radek ; Janouš, Dalibor ; Taufarová, Klára ; Marek, Michal V. ; Hůnová, I. ; Černikovský, L. ; Medlen, A.
Comparison of measured and modelled deposition rate of ozone to spruce forest in locality Bílý Kříž. Estimation of daily changes in deposition and stomatal flux of ozone into spruce forest.
|
|
|
Plant biomass of reclaimed and unreclaimed heps of various age
Dvorščík, Petr ; Frouz, Jan (advisor) ; Cudlín, Pavel (referee)
This thesis has been elaborated as a part of complex succession research on the Velká podkrušnohorská spoil heap near Sokolov (north-west Bohemia, GPS: 50ř13'34.695"N, 12ř42'6.627"E) . The objective of this work is to describe biomass development of reclamational and successional localities on a chronosequence example. Unreclaimed areas are represented by successional spontaneous vegetation with dominant Goat willow (Salix caprea) with an mixture of European aspen (Populus tremula) and European birch (Betula pendula). Reclamation areas are represented by forest planting of Black alder (Alnus glutinosa) mixed with Grey alder (Alnus incana). On this localities was quantify total biomass (above and below ground biomass of herb and tree layer). Data evaluation was performed using allometric equations formed on destructive analysis of the main tree types. There is not statistically significant difference betwen the recovery site methods. Keywords: chronosequence, biomass, succession, reclamation, spoil-heap, allometric
|
|
| |
|
| |
|
| |
guest ::
