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Monitoring of sublingual allergen immunotherapy using serum cytokine levels
Brzáková, Adéla ; Řezáčová, Vladimíra (advisor) ; Andrýs, Ctirad (referee)
Charles University Faculty of Pharmacy in Hradec Králové Department of Biological and Medical Sciences Author: Bc. Adéla Brzáková Supervisor: Mgr. Vladimíra Řezáčová, Ph.D. Title of thesis: Monitoring of sublingual allergen immunotherapy using serum cytokine levels Keywords: allergen immunotherapy, cytokine, ELISA, grass pollen allergy Background: The aim of this thesis was to find out whether there are detectable changes in the levels of IFNγ, IL-4 and IL-5 during allergen immunotherapy and whether these cytokines could thus serve as biomarkers of the efficiency of this treatment. Methods: Selected cytokines were determined using Quantikine® HS ELISA Human IFN-γ Immunoassay, Quantikine® HS ELISA Human IL-4 Immunoassay and Quantikine® ELISA Human IL-5 Immunoassay. The monitored population consisted of 32 polinotics, 19 of which were administered allergen immunotherapy and 13 of which constituted the control group. As part of the follow-up, both groups also continuously recorded the severity of their symptoms using a visual analog scale. Results: In the group of treated patients, a decrease in IL-4, IL-5 and IFNγ concentrations was observed during treatment, but no significant differences in the values of these cytokines were found between patients treated with Oralair and control individuals. Using...
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Monitoring of sublingual allergen immunotherapy using serum cytokine levels
Brzáková, Adéla ; Řezáčová, Vladimíra (advisor) ; Andrýs, Ctirad (referee)
Charles University Faculty of Pharmacy in Hradec Králové Department of Biological and Medical Sciences Author: Bc. Adéla Brzáková Supervisor: Mgr. Vladimíra Řezáčová, Ph.D. Title of thesis: Monitoring of sublingual allergen immunotherapy using serum cytokine levels Keywords: allergen immunotherapy, cytokine, ELISA, grass pollen allergy Background: The aim of this thesis was to find out whether there are detectable changes in the levels of IFNγ, IL-4 and IL-5 during allergen immunotherapy and whether these cytokines could thus serve as biomarkers of the efficiency of this treatment. Methods: Selected cytokines were determined using Quantikine® HS ELISA Human IFN-γ Immunoassay, Quantikine® HS ELISA Human IL-4 Immunoassay and Quantikine® ELISA Human IL-5 Immunoassay. The monitored population consisted of 32 polinotics, 19 of which were administered allergen immunotherapy and 13 of which constituted the control group. As part of the follow-up, both groups also continuously recorded the severity of their symptoms using a visual analog scale. Results: In the group of treated patients, a decrease in IL-4, IL-5 and IFNγ concentrations was observed during treatment, but no significant differences in the values of these cytokines were found between patients treated with Oralair and control individuals. Using...
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The involvement of mitotic kinases AURKA and PLK1 in the oocyte meiotic maturation
Brzáková, Adéla ; Šolc, Petr (advisor) ; Šebková, Nataša (referee)
Aurora A and Plk1 belongs to the extended family of serine/threonine kinases controlling the cell cycle. Both are involved in the control of mitosis and contribute also to the regulation of meiotic division. Although the role of Aurora A in the resumption of meiosis after the first meiotic block seems to be, according to present studies, a bit questionable matter, it is virtually certain that AURKA plays an irreplaceable role in the construction of the spindle. In the oocytes with inhibited (by RNAi or small molecular inhibitors) or overexpressed Aurora A, abnormal spindles with aberrant number of poles or otherwise unusually shaped spindles are created. Disruption of Plk1 function in oocytes also leads to the appearance of damaged spindles. In addition, Plk1, almost certainly, plays a role in the timing of the nuclear membrane breakdown - at GVBD.
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Kinázová signalizace v meióze I savčích oocytů
Brzáková, Adéla ; Šolc, Petr (advisor) ; Dráber, Pavel (referee)
PLK1 belongs to the extended family of serine/threonine kinases controlling the cell cycle. It is well known for its role in the control of mitosis and contributes also to the regulation of meiotic division. On a basis of Live Cell Imaging (LCI) experiments we can describe the phenotype of the oocytes with PLK1 inhibited by small molecular inhibitor BI2536. PLK1 inhibition leads to delayed nuclear envelope breakdown (NEBD) and chromatin condensation (CC) and also causes desynchronization of NEBD and CC; in contrast to control oocytes, PLK1 inhibited oocytes break down their nuclear envelope with chromatin almost fully condensed. Also duration of these two early nuclear events is prolonged in oocytes with inhibited PLK1. In contrast to somatic cells, PLK1 inhibition in mouse oocytes does not prevent assembly of spindle with two distinct poles but affects the final spindle volume. Similar to somatic cells, mouse oocytes with PLK1 inhibited from the beginning of the meiotic maturation stay arrested in metaphase I but in the case of mouse oocytes, this block is not dependent on Spindle Assembly Checkpoint (SAC) persisting activity. When mouse oocytes are synchronized on metaphase I/anaphase I transition by proteasome inhibition and then PLK1 kinase activity is inhibited, about 2/3 of the oocytes stay arrested...
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The involvement of mitotic kinases AURKA and PLK1 in the oocyte meiotic maturation
Brzáková, Adéla ; Šolc, Petr (advisor) ; Šebková, Nataša (referee)
Aurora A and Plk1 belongs to the extended family of serine/threonine kinases controlling the cell cycle. Both are involved in the control of mitosis and contribute also to the regulation of meiotic division. Although the role of Aurora A in the resumption of meiosis after the first meiotic block seems to be, according to present studies, a bit questionable matter, it is virtually certain that AURKA plays an irreplaceable role in the construction of the spindle. In the oocytes with inhibited (by RNAi or small molecular inhibitors) or overexpressed Aurora A, abnormal spindles with aberrant number of poles or otherwise unusually shaped spindles are created. Disruption of Plk1 function in oocytes also leads to the appearance of damaged spindles. In addition, Plk1, almost certainly, plays a role in the timing of the nuclear membrane breakdown - at GVBD.
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