National Repository of Grey Literature 5 records found  Search took 0.00 seconds. 
Posttranslační modifikace proteinů a jejich role v klíčení rostlin
Berka, Miroslav
Protein posttranslational modification represents a complex extension of proteome diversity and provides a transcription-independent control of cellular processes. This thesis "Protein posttranslational modifications and their role in seed germination" briefly reviews major posttranslational modifications, highlights the role of protein phosphorylation in plant regulatory circuits, and summarises enrichment techniques that are employed in phosphoproteomics. The experimental part describes the development and optimisation of methods that can be utilised in a monitoring of protein posttranslational modifications during barley (Hordeum vulgare L.) seed germination, including a tissue fractionation, proteome equalisation and peptide fractionation techniques. Finally, these methods were employed in an analysis of hydrogen peroxide effect on seed germination. In total, over 1,400 proteins were identified in individual fractions, quantitative profiles were established for more than 881 (~60%) and the validated differentially abundant proteins could indicate a potential mechanism behind the peroxide stimulatory effect. Peptides that would correspond to the identified seed phosphoproteins were mostly inaccessible in this experiment and would require optimised targeted analysis.
Abiotické a biotické faktory ovlivňující klíčení rostlin
Berka, Miroslav
Seed germination and early plant development is a crucial phase of plants' life. Multiple internal and external stimuli influence germination progress and have a serious impact on a plant's survival and vitality. Biotic and abiotic stimuli trigger a whole range of changes, both on molecular and developmental levels, but the complex molecular mechanisms regulating these responses are far from being resolved. This thesis reviews the seed germination process and outlines the role of external stimuli in its progress. The experimental part describes the development of a method for seed germination monitoring, provides new insight into the role of hydrogen peroxide in germination, and analyzes effects of cadmium ions, temperature, salt and drought on proteome and metabolome of germinating seeds of Hordeum vulgare. In total, 2000 proteins and 800 metabolites were identified. The analyses revealed over 95 putative abiotic stress markers, including 63 and 36 proteins and metabolites, respectively
A proposal of innovation technology for joining of components for specific purposes
Berka, Miroslav ; Jaroš, Aleš (referee) ; Píška, Miroslav (advisor)
The main goal of the diploma thesis is a proposal of alternative metal sheets joining technology which would replace the current technology of spot welding. Different variants have been proposed, and the one which suits the most, the technology of clinching, was compared to the existing one. The costs have been calculated and following results have been concluded: the return of investment of clinching press is about 2 years. In the case of handheld tongs it is 1 and a quarter of a year. Annual savings of clinching press are 234 485 Kč, and in the case of handheld tongs are 258 019 Kč.
Coated cemented carbides
Berka, Miroslav ; Dvořák, Jaromír (referee) ; Humár, Anton (advisor)
This thesis deals with coated cemented carbides, which are used for the manufac-ture of cutting tools. In the first part are listed and described basic cutting materials. The main part is aimed on the coated cemented carbides - coating methods, manufacturing and properties of coatings. The third part deals with coated cemented carbide product range in the world's leading producer of tools and tool materials.
Náhlé změny klimatu a jejich vliv na pojistný průmysl
Šolcová, Kateřina ; Bílková, Diana (advisor) ; Berka, Miroslav (referee)
Cílem diplomové práce je zhodnotit vliv klimatických změn na pojistný průmysl prostřednictvím analýzy pojistného plnění.Práce se věnuje charakteristikám živelného pojištění, možností predikce vývoje klimatu na pozadí historických zkušeností, rekapituluje velké pojistné škody minulosti a vývoj v uplynulých třiceti letech. Závěr práce je věnován pohledu na situaci v České republice po povodních v roce 2002-jak ovlivnily ekonomiku v tomto roce a jaký dopad měly na pojistný průmysl v ČR.

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