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Speciation analysis of selected mercury compounds using combined technique HPCL-EcMCVG-QFAAS
Leštinová, Veronika ; Červený, Václav (advisor) ; Spěváčková, Věra (referee)
Today, the information about total mercury content in a sample is not enough to know because of different toxicity of various species like inorganic forms such as Hg+ , Hg+II and organic bonded mercury in Methyl-mercury, Ethyl-mercury or Phenyl-mercury. Therefore a speciation analysis is needed to do. The aim of this diploma thesis was to develop and validate a new method for speciation determination of selected mercury compounds. This method comprises a connection of high performance liquid chromatography, electrochemical mercury cold vapor generation as a postcolumn derivatization technique and atomic absorption spectrometer. Using this on-line connection of mentioned techniques, an efficient separation of Hg+II and methylmercury was achieved. Reached limits of detection were 0.3 µgml-1 and 0.5 µgml-1 for Hg+II and methylmercury, respectively. At the end of experimental work, proposed hyphenated technique HPLC- EcMCVG-QFAAS was used for determination of these two mercury compounds in six real tap water samples (Bílý potok, Bílina, Labe, Vltava, Botič, pond Kovošrot). In Bílý potok, inorganic mercury (Hg+II ) was identified on the base of identical of retention time compared to standard. Standard addition method was used in both FIA and HPLC arrangements and determined content of Hg+II was (0.4...
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