National Repository of Grey Literature 11 records found  1 - 10next  jump to record: Search took 0.01 seconds. 
Simple route of caspase-3 FRET sensor synthesis using “click chemistry”
Lišková, Marcela ; Křenková, Jana ; Klepárník, Karel ; Pazdera, P. ; Foret, František
Programmed cell death or apoptosis is regulated process of cell suicide. The central role in apoptosis play cysteine proteases called caspases. Caspases recognize tetra-peptide sequences Asp-Glu-Val-Asp (DEVD) on their substrates and hydrolyze peptide bonds after aspartic acid residues. Various techniques for the determination of caspase-3 are commercially available e.g. Enzyme Linked Immuno-Sorbent Assay (ELISA), Western blotting or flow cytometric analysis. The products of the cleavage can be detected by spectrophotometry, fluorimetry, chemiluminescence (CL) or ELISA. In this work, we suggested fluorescent sensor based on easily prepared Förster Resonance Energy Transfer (FRET). We use very simple chemistry called “click”. This type of chemistry takes advantages of quickness, simplicity and cheapness. “Click chemistry” is based on usage of various functional group and cross-linkers to combine individual molecules together.
Multi-step synthesis of caspase-3 sensor based on Förster resonance energy transfer
Lišková, Marcela ; Klepárník, Karel ; Pazdera, P. ; Foret, František
Programmed cell death or apoptosis is regulated process of cell suicide. The central role in apoptosis play cysteine proteases called caspases. Caspases recognize tetra-peptide sequences Asp-Glu-Val-Asp (DEVD) on their substrates and hydrolyze peptide bonds after aspartic acid residues. Various techniques for the determination of caspase-3 are commercially available e.g. Enzyme Linked Immuno-Sorbent Assay (ELISA), Western blotting or flow cytometric analysis. The products of the cleavage can be detected by spectrophotometry, fluorimetry, chemiluminescence (CL) or ELISA. In this work, we suggested fluorescent sensor based on Förster Resonance Energy Transfer (FRET) to determine caspase-3 in cell nucleus or cytoplasm by apoptosis for very fast analysis by fluorescence microscopy without sample destroying.
Caspase 3 chemiluminiscence activity determination in apoptotic cells and design of Caspase 3 sensor
Lišková, Marcela ; Klepárník, Karel ; Pazdera, P. ; Foret, František
Apoptosis, or programmed cell death, is an essential physiological process that plays a critical role in development of tumor or autoimmune disorders. In some instances knowledges of this pathway can be used for inhibit or prevent cancer development and other diseases. One of the earliest and most consistent observed features of apoptosis is the induction of a series of cytosolic proteases – caspases.
Sensor based on Förster resonance energy transfer with quantum dot
Lišková, Marcela ; Datinská, Vladimíra ; Klepárník, Karel ; Foret, František
Förster resonance energy transfers (FRET) are based on the nonradiative transfer of energy between a donor and acceptor dye in the distance 1-10 nm. With respect to this, quantum dots (QDs) with a high absorbance can serve as suitable donors of energy in FRET. Here, we present architectures of two sensors based on QD conjugates useful in imaging and diagnostics of cancer. One sensor, based on an oligonucleotide conjugate, can be used for the detection of complementary oligonucletide chains. The other was designed for the detection of enzymes inside cells.
A simple device for quantitation of Caspase 3 in individual apoptotic embryonic cells
Hegrová, Jitka ; Klepárník, Karel ; Přikryl, Jan ; Lišková, Marcela ; Matalová, Eva ; Foret, František
A newly developed system for the determination of Caspase 3 activity in individual apoptotic embryonic cells is described. Caspase 3, a cysteine-aspartic acid protease activated in the apoptotic pathway, plays an essential role in the programmed cell death. Moreover, failure of apoptosis is one of the main contributions to tumor development.
Preparation of highly luminescent CdTe quantum dots and its probes
Voráčová, Ivona ; Lišková, Marcela ; Patrmanová, M. ; Klepárník, Karel ; Foret, František
Luminescence probes consisting of quantum dots conjugated with macrocyclic ligand complex were characterized by CE-LIF.
Immunofluorescent labeling of tissues and free cells via QD-protein conjugates
Lišková, Marcela ; Voráčová, Ivona ; Hezinová, Věra ; Klepárník, Karel ; Foret, František
Preparation of quantum dots and using their QD-protein conjugates in immunofluorescent labeling of cells and tissues. For conjugation was used method viaEDC/sulfo-NHS zero-length cross-linkers. We linked QD with annexin V, anti-CD3 and anti-PCNA. We labeled T-lymphocytes, apoptotic lymphocyte, mouse duodenum tissue and tumor tissue.
Preparation, physical-chemical properties and utilization of nanoparticles in bioanalysis
Svobodová, Ivona ; Hezinová, Věra ; Lišková, Marcela ; Přikryl, Jan ; Maděránková, D. ; Klepárník, Karel ; Foret, František
The possibilities of application of nanoparticles in bioanalysis were studied. Applications of quantum dots as a fluorescent markers, silver nanoparticles in SERS and conjucation of gold nanoparticles with antibodies were shown.
Application of nanoparticles in fluorescence microscopy and raman spectrometry
Přikryl, Jan ; Svobodová, Ivona ; Hezinová, Věra ; Lišková, Marcela ; Maděránková, D. ; Klepárník, Karel ; Foret, František
This contribution is devoted to application of quantum dots as perspective luminescence labels in fluorescence microscopy (epifocal and TIR fluorescence microscopy) and application of silver nanoparticles in surface-enhanced Raman spectroscopy.
CdTe kvantové tečky - příprava, fyzikálně-chemické vlastnosti a aplikace jako fluorescenční značky
Hezinová, Věra ; Svobodová, Ivona ; Lišková, Marcela ; Přikryl, Jan ; Klepárník, Karel ; Foret, František
The set of different sizes of CdTe nanoparticles coated with MPA was synthetized and characterized by fluorescence spectra and lifetimes. Their application as a cell fluorescent markers was tested.

National Repository of Grey Literature : 11 records found   1 - 10next  jump to record:
See also: similar author names
8 LIŠKOVÁ, Marie
9 LIŠKOVÁ, Markéta
6 LIŠKOVÁ, Michaela
4 LIŠKOVÁ, Monika
4 Lisková, Monika
3 Lišková, Magdalena
3 Lišková, Magdaléna
8 Lišková, Marie
9 Lišková, Markéta
2 Lišková, Marta
11 Lišková, Martina
6 Lišková, Michaela
1 Lišková, Michala
1 Lišková, Miloslava
4 Lišková, Monika
8 Lísková, Marie
1 Líšková, Marianna
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