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Effect of cytokinin deficiency on oxidative damage and antioxidant defence during ageing and abiotic stress
Mýtinová, Zuzana ; Wilhelmová, Naďa (vedoucí práce) ; Ryšlavá, Helena (oponent) ; Prášil, Ilja (oponent)
Pouze několik prací se zabývó rodí|em mtioxi&ční ochay m€zi listy a kořeny (Cryprset al.,2002; Dixit et al.,200|: Liu d Huang' 2000).v tetopnácise o@věď dcivit AoE na stresv listech a kďeirech vášinou lišila"obecně lze říci' žev listeohbyla alrtivitaAPX vyššía dctivita cAT niŽšíve srovnánís kďeny u obou t5půrostlin, a to běhe,lrrnestesoých i *rcswýcn poamaac Aktivita SoD byla niXi v kořenect wT ďi koffro|níchporůrrínkach6 zmolení u ostabÍchstreslbyly *tivity SoD srovnde|név listech i kďenec,h.Attiúta GR byla zvýšenáv kďenech WT oproti lisům' a to při zasolenía suchrr. ZÁvěr l) Listy trmsg€mí€h rostlin se sníženouh|adinou CK ve srovnfoí s kontrolorr strfonou pomateji. AtcKx2 maji v nejstaršíchlistech vyššíobsah Ch| proteinůa takévyšší fotosyntetiďou účinnost. 2) lE tt nejň|adšíoh listů AtcKx2 by|a stmovena zr.ýš€ní mtioxidační o&ma (enzymatickái neenzynďická). 3\ zwšffi mtioxidační ocbÍana m]ůŽebýt inórkováoa jako kompenzace poklesu koncenÚaceCK považovanýchzapotencir{''lníneenzymďiclé antioxidanty,neboto může být ólsles oxidačníhostr€su způsob€néhonedosatk€n CKjiž v mlaóých listeoh. 4) Je takémofué'že vyššlantioxidačníochranav list€ch s nedo$*em cK můŽ'epřispívat . ke 4malenému strírnutíav dusledkutohoopožděnému&isfutrr senescence. 5) @ověď aktivit AoE na jednotlivé sÚesy s€ v listech a kořerech liší mezi...
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Regulation of phosphoenolpyruvate carboxylase in tobacco plants during potyviral infection
Müller, Karel ; Ryšlavá, Helena (vedoucí práce) ; Vaňková, Radomíra (oponent) ; Luhová, Lenka (oponent)
Charles University in Prague Faculty of Science, Department of Biochemistry Regulation of phosphoenolpyruvate carboxylase in tobacco plants during potyviral infection Regulace fosfoenolpyruvátkarboxylasy v rostlinách tabáku během virové infekce Karel Müller Supervisor: RNDr. Helena Ryšlavá CSc. Prague, 2008 2 Table of contents / Obsah Introduction........................................................................................................................................ 3 Aim of the work................................................................................................................................. 4 Results and discussion ....................................................................................................................... 5 Isolation of PEPC from tobacco leaves ......................................................................................... 5 Characterization of tobacco PEPC................................................................................................. 5 Regulation of tobacco PEPC during viral infection....................................................................... 7 Effect of cytokinins in tobacco leaves during stress.................................................................... 10...
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Studium regulace NADP - malátdehydrogenasy (dekarboxylační) a vliv stresu na tento enzym
Doubnerová, Veronika ; Ryšlavá, Helena (vedoucí práce) ; Wilhelmová, Naďa (oponent) ; Kizek, René (oponent)
4. Conclusions l. Non-photosynthetic cytosolic isoform of NADP-mďic ilZ-oe (oxaloacetate decarboxylating) (NADp-ME; EC r.l.l.4o) was isolated from tobacco (Nicoriana tabacun L.) leaves. Specific aďiviý of the obtained enz5me preparation was 0.95pmol.miď|.mg l, relďive molecular mass of one subr'nit of tetramer approximatety 67000' pI 5'5 and pH optimum 7.r - 7.4. Kinetic constants of this isoform were determined for L-ma|ate and NADP+ in the presence of various cofactors Mď*, Mo*,co2', Ni2*, similarly the dependencies ofreaction Íates on the ooncentration ofdivalent metal ions were observed- The dependence of reaction rate on Mn2+ concentration wassigmoidal with strong positive cooperativiý and high value of Hill coefficient 7.5. -2r - J. 4. Summarv ofPh.D.Thesis 2. The kinetic meohanism of two-substate reaďion catalyzed by NADP.ME ůomtobacco leaves was determined as ordered sequmtial. The regulation of the NADP-ME purified ůomtobacco leaves occurs predominant|y in the prsence of macmergic compounds (GTP, ATP and ADP), sligbtly by metabolites of glymlysis. Biotic stress caused by potato yirus f G1;Y) (not only strain PWNN but also PWo) increased NADP.ME activiý in N. tabacum L. |eaves, stems and roots during the infeďion. Milder PVYo caused tower increase of NADP-ME aďivity than necrotic isolate of PVYNN....
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