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Analysis of autenthicity of food products with fruit component
Prachárová, Adriana ; Mikulíková, Renata (oponent) ; Márová, Ivana (vedoucí práce)
The aim of this thesis was to determine the authenticity of fruit food for infants using molecular and instrumental methods. In the experimental part, plant DNA isolations from fruit leaves (peaches, apricots, plums and apples) and bananas were performed. Further, DNA was isolated also from five commercial products, and from model mixtures that were prepared in terms of content identical to the commercial mixtures. The isolated DNA was characterized and verified by qPCR with plant DNA-specific ITS2 primers. Three triple primer pairs were selected, and their specificity was evaluated when performing multiplex PCR. This method makes it possible to detect more types of fruit in one reaction, reducing the economic and time requirements for detection. As none of the selected primer pairs were sufficiently specific for the apricot, the evidence from the plum and peach was further realized using duplex PCR. High resolution melting curve analysis was used for better DNA type recognition. Subsequently, agarose gel electrophoresis was performed to analyse the fragment lengths. Furthermore, experiments have been made to identify some specific phenolic substances in commercial and model fruit mixtures by HPLC. Since phenolic substances are degradable under unsuitable storage conditions, the presence of individual compounds was not detected by this method.
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Characterization of some super-foods as components of smoothie drinks
Prachárová, Adriana ; Vysoká, Marie (oponent) ; Márová, Ivana (vedoucí práce)
This bachelor thesis deals with properties of selected fruits and seeds of superfoods, the introduction of their biologically active substances and comparison of their nutritional properties. The theoretical part focuses on characterizing the fruit itself (pineapple, banana, blueberry, kiwi, raspberry and orange) and seeds (chia seeds, poppy seeds and almonds). It is focused on characterization of bioactive substances contained in them, such as polyphenols, flavonoids, antioxidant activity, vitamin C, vitamin E and fatty acids. Further, used methods are described. In the experimental part, the content of the antioxidant activity and biological effects in selected fruits and seeds was measured and concentrations was calculated per 1 g of dry matter. Finally, a sensory analysis consisting of eighteen fruit and seed combinations is introduced. The aim of the thesis was to find out which of tested combinations are nutritionally high and, simultaneously, acceptable for consumer.
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Analysis of autenthicity of food products with fruit component
Prachárová, Adriana ; Mikulíková, Renata (oponent) ; Márová, Ivana (vedoucí práce)
The aim of this thesis was to determine the authenticity of fruit food for infants using molecular and instrumental methods. In the experimental part, plant DNA isolations from fruit leaves (peaches, apricots, plums and apples) and bananas were performed. Further, DNA was isolated also from five commercial products, and from model mixtures that were prepared in terms of content identical to the commercial mixtures. The isolated DNA was characterized and verified by qPCR with plant DNA-specific ITS2 primers. Three triple primer pairs were selected, and their specificity was evaluated when performing multiplex PCR. This method makes it possible to detect more types of fruit in one reaction, reducing the economic and time requirements for detection. As none of the selected primer pairs were sufficiently specific for the apricot, the evidence from the plum and peach was further realized using duplex PCR. High resolution melting curve analysis was used for better DNA type recognition. Subsequently, agarose gel electrophoresis was performed to analyse the fragment lengths. Furthermore, experiments have been made to identify some specific phenolic substances in commercial and model fruit mixtures by HPLC. Since phenolic substances are degradable under unsuitable storage conditions, the presence of individual compounds was not detected by this method.
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Characterization of some super-foods as components of smoothie drinks
Prachárová, Adriana ; Vysoká, Marie (oponent) ; Márová, Ivana (vedoucí práce)
This bachelor thesis deals with properties of selected fruits and seeds of superfoods, the introduction of their biologically active substances and comparison of their nutritional properties. The theoretical part focuses on characterizing the fruit itself (pineapple, banana, blueberry, kiwi, raspberry and orange) and seeds (chia seeds, poppy seeds and almonds). It is focused on characterization of bioactive substances contained in them, such as polyphenols, flavonoids, antioxidant activity, vitamin C, vitamin E and fatty acids. Further, used methods are described. In the experimental part, the content of the antioxidant activity and biological effects in selected fruits and seeds was measured and concentrations was calculated per 1 g of dry matter. Finally, a sensory analysis consisting of eighteen fruit and seed combinations is introduced. The aim of the thesis was to find out which of tested combinations are nutritionally high and, simultaneously, acceptable for consumer.
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