999C1a:GA303/02/0433 - Výsledky hledání - Digitální repozitář

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	Characterization of peptide proteinase inhibitors isolated from boar seminal plasma Jelínková, Petra ; Tichá, M. ; Jonáková, Věra Most of proteins of boar seminal plasma are present in this medium under physiological conditions in the form of aggregates and not of monomers. In the present communication we have shown that not only spermadhesins, but also proteinase inhibitors participate in the formation of associated complexes. Proteinase inhibitors were found after gel chromatographic separation mainly in the fraction with the lowest relative molecular mass. Two different types of serine proteinase inhibitors were isolated from boar seminal plasma ů (i) glycosylated inhibitor with Mr 12 kDa, (ii) non-glycosylated sperm-associated acrosin inhibitor with Mr 8 kDa, and a new type of low molecular weight protein that is probably composed of two subunits coupled by the -S-S- bridge. The proteinase inhibitor with Mr 8 kDa was proved to form a heterodimer with AQN 1 spermadhesin under physiological conditions, whereas inhibitor with Mr 12 kDa forms homodimers. Úplný záznam
	Role of sperm surface proteins in gamete binding Jonáková, Věra ; Tichá, M. Mammalian fertilization includes highly regulated biochemical interactions between complementary molecules located on the surface of both gametes. Sperm surface proteins play various roles in the fertilization process. Úplný záznam
	ISOLATION AND CHARACTERIZATION OF MANNAN-BINDING PROTEINS FROM BOAR SEMINAL PLASMA Jelínková, Petra ; Liberda, J. ; Ryšlavá, H. ; Tichá, M. ; Jonáková, Věra The mannan-binding proteins of boar seminal plasma may be involved in the formation of the sperm oviductal reservoir in pig. The ability of these proteins to interact both with the complex of D-mannose-containing saccharide structures and heparin may also play an important role in the sperm release from the oviductal reservoir and in the capacitation process. Úplný záznam
	Protein Characterization of Boar Epididymal Fluid Maňásková, Pavla ; Tichá, M. ; Jonáková, Věra Úplný záznam
	Study of aggregated forms of proteinase inhibitors from boar seminal plasma by gel chromatography Jelínková, Petra ; Maňásková, Pavla ; Tichá, M. ; Jonáková, Věra Most of proteins in seminal plasma exist under physiological conditions in the form of aggregates.The study of the presence of proteinase inhibitors in different associated forms of boar seminal plasma proteins is the subject of our present communicat. Úplný záznam
	Study of aggregated forms of proteinase inhibitors from boar seminl plasma by gel chromatography Jelínková, Petra ; Maňásková, Pavla ; Tichá, M. ; Jonáková, Věra Most of proteins in seminal plasma exist under physiological conditions in the form of aggregates. The study of the presence of proteinase inhibitosr in different associated forms of boar seminal plasma proteins is the subject of our present communication. Úplný záznam
	Separation of human seminal plasma proteins by selected chromatographic and electrophoretic methods Kraus, Marek ; Tichá, M. ; Jonáková, Věra We concentrated on proteins related to boar spermadhesins, which have not been identified, yet in human seminal plasma. We studied the cross-reactivity of antibodies against AQN and AWN boar spermadhesins with human seminal proteins. Úplný záznam
	Proteins from boar prostate secretion: their isolation and characterization Maňásková, Pavla ; Ryšlavá, H. ; Tichá, M. ; Jonáková, Věra Prostate proteins were separated by affinity chromatography on immobilized heparin. Major identified components of prostate secretion:beta-microseminoprotein, serotransferin, serum albumin, PSP I and II spermadhesins. Úplný záznam
	An approach for isolation of human seminal plasma proteins Kraus, Marek ; Tichá, M. ; Jonáková, Věra Human seminal plasma contains various type of protein. For their isolation were used these methods: affinity chromatography, gel chromatography, HPLC etc. The cross-reactivity of antibodies against boar seprmadhesing AQN and AWN was studied with human seminal proteins. Úplný záznam
	Properties of serine proteinase inhibitors from boar seminal plasma Jelínková, Petra ; Maňásková, Pavla ; Tichá, M. ; Jonáková, Věra Inhibitors of proteinases are important components of seminal plasma. Their biological function in seminal plasma is to protect protein components of seminal plasma and male and female reproductive tracts against proteolytic degradation. My work is concentrated on characterization of inihibitors found in seminal plasma. Úplný záznam

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